ID DGAT1_DICDI Reviewed; 617 AA. AC Q55BH9; DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot. DT 16-JUN-2009, sequence version 2. DT 06-JUL-2016, entry version 70. DE RecName: Full=Diacylglycerol O-acyltransferase 1; DE EC=2.3.1.20; GN Name=dgat1; ORFNames=DDB_G0271342; OS Dictyostelium discoideum (Slime mold). OC Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium. OX NCBI_TaxID=44689; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=AX4; RX PubMed=12097910; DOI=10.1038/nature00847; RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., RA Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., RA Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., RA Platzer M., Rosenthal A., Noegel A.A.; RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum."; RL Nature 418:79-85(2002). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=AX4; RX PubMed=15875012; DOI=10.1038/nature03481; RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., RA Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., RA Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., RA Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., RA Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., RA Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., RA Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., RA Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., RA Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., RA Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., RA Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., RA Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., RA Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., RA Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., RA Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., RA Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., RA Kuspa A.; RT "The genome of the social amoeba Dictyostelium discoideum."; RL Nature 435:43-57(2005). CC -!- FUNCTION: Catalyzes the terminal and only committed step in CC triacylglycerol synthesis by using diacylglycerol and fatty acyl CC CoA as substrates. {ECO:0000250}. CC -!- CATALYTIC ACTIVITY: Acyl-CoA + 1,2-diacylglycerol = CoA + CC triacylglycerol. CC -!- PATHWAY: Lipid metabolism; glycerolipid metabolism. CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. CC -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family. CC Sterol o-acyltransferase subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AAFI02000006; EAL71801.2; -; Genomic_DNA. DR RefSeq; XP_645633.2; XM_640541.2. DR STRING; 44689.DDB0304727; -. DR PaxDb; Q55BH9; -. DR EnsemblProtists; DDB0304727; DDB0304727; DDB_G0271342. DR GeneID; 8617825; -. DR KEGG; ddi:DDB_G0271342; -. DR dictyBase; DDB_G0271342; dgat1. DR eggNOG; KOG0380; Eukaryota. DR eggNOG; COG5056; LUCA. DR InParanoid; Q55BH9; -. DR KO; K11155; -. DR OMA; QQWMVPI; -. DR PhylomeDB; Q55BH9; -. DR Reactome; R-DDI-1482883; Acyl chain remodeling of DAG and TAG. DR Reactome; R-DDI-75109; Triglyceride Biosynthesis. DR UniPathway; UPA00230; -. DR PRO; PR:Q55BH9; -. DR Proteomes; UP000002195; Chromosome 2. DR Proteomes; UP000002195; Unassembled WGS sequence. DR GO; GO:0005783; C:endoplasmic reticulum; IDA:dictyBase. DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0003846; F:2-acylglycerol O-acyltransferase activity; ISS:dictyBase. DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IBA:GO_Central. DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW. DR GO; GO:0019432; P:triglyceride biosynthetic process; IMP:dictyBase. DR InterPro; IPR027251; Diacylglycerol_acylTrfase1. DR InterPro; IPR004299; MBOAT_fam. DR InterPro; IPR014371; Oat_ACAT_DAG_ARE. DR PANTHER; PTHR10408; PTHR10408; 1. DR PANTHER; PTHR10408:SF7; PTHR10408:SF7; 1. DR Pfam; PF03062; MBOAT; 1. DR PIRSF; PIRSF000439; Oat_ACAT_DAG_ARE; 1. PE 3: Inferred from homology; KW Acyltransferase; Complete proteome; Endoplasmic reticulum; KW Glycerol metabolism; Lipid biosynthesis; Lipid metabolism; Membrane; KW Reference proteome; Transferase; Transmembrane; Transmembrane helix. FT CHAIN 1 617 Diacylglycerol O-acyltransferase 1. FT /FTId=PRO_0000377488. FT TRANSMEM 217 237 Helical. {ECO:0000255}. FT TRANSMEM 254 274 Helical. {ECO:0000255}. FT TRANSMEM 306 326 Helical. {ECO:0000255}. FT TRANSMEM 399 419 Helical. {ECO:0000255}. FT TRANSMEM 449 469 Helical. {ECO:0000255}. FT TRANSMEM 520 540 Helical. {ECO:0000255}. FT TRANSMEM 545 565 Helical. {ECO:0000255}. FT TRANSMEM 570 590 Helical. {ECO:0000255}. FT COMPBIAS 18 35 Poly-Gln. FT COMPBIAS 96 104 Poly-Asn. FT COMPBIAS 153 158 Poly-His. FT COMPBIAS 159 177 Poly-Asn. FT ACT_SITE 532 532 {ECO:0000255}. SQ SEQUENCE 617 AA; 71946 MW; DCD8FD193A3023D7 CRC64; MEPIPPSNGN KNNSMDKQPQ QPQQPQQQQQ QQQQQRRDQR NSKLNELNET ERVRNRFISH EFHKLDRTKS RIDAPKISFS DSESESDSEF FLAKRNTNNN NQNNTSPTFS SANGKQSNLT QRKINTQIQS KQPTNNNVQP LTDDEGTINH SNHHHHHHNQ NNNGNNNNNN NNNNNNNKIS TPPKQEEKMT MNGLFTLRPS ILSSESNGSS YRGFLNLLLI LLITASFRLV ILNHLLYGIR INLDLYKISE YHRWPGVMIS LMINLFIIAA YLIEKAAAKQ LLPDRICYLL RIINCAAVII VPSGSIIAFS PNPASGIIVM ILICTFSMKI ISYAYENSKQ RKLNPDNKKF VIDPTNTSIY PNNLSLRSTY WFMLVPTLVY QLSYPRSPKI RKGYLLRRIV EALSLSLLIL WMVNQYMLPL VQNSIEPLEK IDIVLIVERI MKLSLPNLYV WLLGFYVFFH LYLNIVAEIT RFGDREFYRD WWNSTGLDYF WRTWNMPVHH WMVVLIYTPM RRRGFSKNMG YFMCFFVSAI FHELVISIPF HSLKLWGFFG IMSQMVLIAL TKNLMNGRNL GNVIFWISIV LGQPLVVLLY YRNFVLENPE WYRNVEPPTS PPVMPFY //