ID DGAT1_DICDI Reviewed; 617 AA. AC Q55BH9; DT 16-JUN-2009, integrated into UniProtKB/Swiss-Prot. DT 16-JUN-2009, sequence version 2. DT 13-OCT-2009, entry version 24. DE RecName: Full=Diacylglycerol O-acyltransferase 1; DE EC=2.3.1.20; GN Name=dgat1; ORFNames=DDB_G0271342; OS Dictyostelium discoideum (Slime mold). OC Eukaryota; Amoebozoa; Mycetozoa; Dictyosteliida; Dictyostelium. OX NCBI_TaxID=44689; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=AX4; RX MEDLINE=22092622; PubMed=12097910; DOI=10.1038/nature00847; RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., RA Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., RA Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., RA Platzer M., Rosenthal A., Noegel A.A.; RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum."; RL Nature 418:79-85(2002). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=AX4; RX PubMed=15875012; DOI=10.1038/nature03481; RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., RA Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., RA Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., RA Bankier A.T., Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., RA Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., RA Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., RA Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., RA Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., RA Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., RA Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., RA Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., RA Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., RA Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., RA Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., RA Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., RA Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., RA Kuspa A.; RT "The genome of the social amoeba Dictyostelium discoideum."; RL Nature 435:43-57(2005). CC -!- FUNCTION: Catalyzes the terminal and only committed step in CC triacylglycerol synthesis by using diacylglycerol and fatty acyl CC CoA as substrates (By similarity). CC -!- CATALYTIC ACTIVITY: Acyl-CoA + 1,2-diacylglycerol = CoA + CC triacylglycerol. CC -!- PATHWAY: Lipid metabolism; glycerolipid metabolism. CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass CC membrane protein (By similarity). CC -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family. CC Sterol o-acyltransferase subfamily. CC -!- SEQUENCE CAUTION: CC Sequence=EAL71801.1; Type=Erroneous gene model prediction; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AAFI02000006; EAL71801.1; ALT_SEQ; Genomic_DNA. DR RefSeq; XP_645633.1; -. DR STRING; Q55BH9; -. DR GeneID; 3396843; -. DR GenomeReviews; CM000151_GR; dgat1. DR KEGG; ddi:DDBDRAFT_0202877; -. DR dictyBase; DDB_G0271342; dgat1. DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW. DR GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-SubCell. DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:EC. DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW. DR GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProtKB-KW. DR InterPro; IPR004299; MBOAT_fam. DR Pfam; PF03062; MBOAT; 1. PE 3: Inferred from homology; KW Acyltransferase; Complete proteome; Endoplasmic reticulum; KW Glycerol metabolism; Lipid metabolism; Lipid synthesis; Membrane; KW Transferase; Transmembrane. FT CHAIN 1 617 Diacylglycerol O-acyltransferase 1. FT /FTId=PRO_0000377488. FT TRANSMEM 217 237 Potential. FT TRANSMEM 254 274 Potential. FT TRANSMEM 306 326 Potential. FT TRANSMEM 399 419 Potential. FT TRANSMEM 449 469 Potential. FT TRANSMEM 520 540 Potential. FT TRANSMEM 545 565 Potential. FT TRANSMEM 570 590 Potential. FT COMPBIAS 18 35 Poly-Gln. FT COMPBIAS 96 104 Poly-Asn. FT COMPBIAS 153 158 Poly-His. FT COMPBIAS 159 177 Poly-Asn. FT ACT_SITE 532 532 Potential. SQ SEQUENCE 617 AA; 71946 MW; DCD8FD193A3023D7 CRC64; MEPIPPSNGN KNNSMDKQPQ QPQQPQQQQQ QQQQQRRDQR NSKLNELNET ERVRNRFISH EFHKLDRTKS RIDAPKISFS DSESESDSEF FLAKRNTNNN NQNNTSPTFS SANGKQSNLT QRKINTQIQS KQPTNNNVQP LTDDEGTINH SNHHHHHHNQ NNNGNNNNNN NNNNNNNKIS TPPKQEEKMT MNGLFTLRPS ILSSESNGSS YRGFLNLLLI LLITASFRLV ILNHLLYGIR INLDLYKISE YHRWPGVMIS LMINLFIIAA YLIEKAAAKQ LLPDRICYLL RIINCAAVII VPSGSIIAFS PNPASGIIVM ILICTFSMKI ISYAYENSKQ RKLNPDNKKF VIDPTNTSIY PNNLSLRSTY WFMLVPTLVY QLSYPRSPKI RKGYLLRRIV EALSLSLLIL WMVNQYMLPL VQNSIEPLEK IDIVLIVERI MKLSLPNLYV WLLGFYVFFH LYLNIVAEIT RFGDREFYRD WWNSTGLDYF WRTWNMPVHH WMVVLIYTPM RRRGFSKNMG YFMCFFVSAI FHELVISIPF HSLKLWGFFG IMSQMVLIAL TKNLMNGRNL GNVIFWISIV LGQPLVVLLY YRNFVLENPE WYRNVEPPTS PPVMPFY //