ID Q53HW5_HUMAN Unreviewed; 782 AA. AC Q53HW5; DT 24-MAY-2005, integrated into UniProtKB/TrEMBL. DT 24-MAY-2005, sequence version 1. DT 07-APR-2021, entry version 82. DE RecName: Full=DNA excision repair protein ERCC-3 {ECO:0000256|ARBA:ARBA00015423}; DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551}; DE AltName: Full=General transcription and DNA repair factor IIH helicase subunit XPB {ECO:0000256|ARBA:ARBA00014343}; DE Flags: Fragment; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. OX NCBI_TaxID=9606 {ECO:0000313|EMBL:BAD96185.1}; RN [1] {ECO:0000313|EMBL:BAD96185.1} RP NUCLEOTIDE SEQUENCE. RC TISSUE=Adipose tissue {ECO:0000313|EMBL:BAD96185.1}; RX PubMed=8125298; DOI=10.1016/0378-1119(94)90802-8; RA Maruyama K., Sugano S.; RT "Oligo-capping: a simple method to replace the cap structure of eukaryotic RT mRNAs with oligoribonucleotides."; RL Gene 138:171-174(1994). RN [2] {ECO:0000313|EMBL:BAD96185.1} RP NUCLEOTIDE SEQUENCE. RC TISSUE=Adipose tissue {ECO:0000313|EMBL:BAD96185.1}; RX PubMed=9373149; DOI=10.1016/S0378-1119(97)00411-3; RA Suzuki Y., Yoshitomo K., Maruyama K., Suyama A., Sugano S.; RT "Construction and characterization of a full length-enriched and a 5'-end- RT enriched cDNA library."; RL Gene 200:149-156(1997). RN [3] {ECO:0000313|EMBL:BAD96185.1} RP NUCLEOTIDE SEQUENCE. RC TISSUE=Adipose tissue {ECO:0000313|EMBL:BAD96185.1}; RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., RA Tanaka A., Yokoyama S.; RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; CC Evidence={ECO:0000256|ARBA:ARBA00001665}; CC -!- SIMILARITY: Belongs to the helicase family. RAD25/XPB subfamily. CC {ECO:0000256|ARBA:ARBA00006637}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK222465; BAD96185.1; -; mRNA. DR PeptideAtlas; Q53HW5; -. DR OMA; SQEWGLM; -. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC. DR GO; GO:0006289; P:nucleotide-excision repair; IEA:InterPro. DR GO; GO:0006367; P:transcription initiation from RNA polymerase II promoter; IEA:InterPro. DR InterPro; IPR032438; ERCC3_RAD25_C. DR InterPro; IPR006935; Helicase/UvrB_N. DR InterPro; IPR014001; Helicase_ATP-bd. DR InterPro; IPR001650; Helicase_C. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR001161; XPB/Ssl2. DR InterPro; IPR032830; XPB/Ssl2_N. DR Pfam; PF16203; ERCC3_RAD25_C; 1. DR Pfam; PF13625; Helicase_C_3; 1. DR Pfam; PF04851; ResIII; 1. DR SMART; SM00487; DEXDc; 1. DR SMART; SM00490; HELICc; 1. DR SUPFAM; SSF52540; SSF52540; 2. DR TIGRFAMs; TIGR00603; rad25; 1. DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1. DR PROSITE; PS51194; HELICASE_CTER; 1. PE 2: Evidence at transcript level; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840}; KW DNA-binding {ECO:0000256|ARBA:ARBA00023125}; KW Hydrolase {ECO:0000256|ARBA:ARBA00022801}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}. FT DOMAIN 327..488 FT /note="Helicase ATP-binding" FT /evidence="ECO:0000259|PROSITE:PS51192" FT DOMAIN 542..702 FT /note="Helicase C-terminal" FT /evidence="ECO:0000259|PROSITE:PS51194" FT REGION 1..51 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 220..241 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 1..22 FT /note="Polyampholyte" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 220..239 FT /note="Polar" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT NON_TER 1 FT /evidence="ECO:0000313|EMBL:BAD96185.1" SQ SEQUENCE 782 AA; 89149 MW; 8789BBFD5E1F4B6E CRC64; MGKRDRADRD KKKSRKRHYE DEEDDEEDAP GNDPQEAVPS AAGKQVDESG TKVDEYGAKD YRLQMPLKDD HTSRPLWVAP DGHIFLEAFS PVYKYAQDFL VAIAEPVCRP THVHEYKLTA YSLYAAVSVG LQTSDITEYL RKLSKTGVPD GIMQFIKLCT VSYGKVKLVL KHNRYFVESC HPDVIQHLLQ DPVIRECRLR NSEGEATELI TETFTSKSAI PKTAESSGGP STSRVTDPQG KSDIPMDLFD FYEQMDKDEE EEEETQTVSF EVKQEMIEEL QKRCIHLEYP LLAEYDFRND SVNPDINIDL KPTAVLRPYQ EKSLRKMFGN GRARSGVIVL PCGAGKSLVG VTAACTVRKR CLVLGNSAVS VEQWKAQFKM WSTIDDSQIC RFTSDAKDKP IGCSVAISTY SMLGHTTKRS WEAERVMEWL KTQVWGLMIL DEVHTIPAKM FRRVLTIVQA HCKLGLTATL VREDDKIVDL NFLIGPKLYE ANWMELQNNG YIAKVQCAEV WCPMSPEFYR EYVAIKTKKR ILLYTMNPNK FRACQFLIKF HERRNDKIIV FADNVFALKE YAIRLNKSYI YGPTSQGERM QILQNFKHNP KINTIFISKV GDTSFDLPEA NVLIQISSHG GSRRQEAQRL GRVLRAKKGM VAEEYNAFFY SLVSQDTQEM AYSTKRQGFL VDQGYSFKVI TKLAGMEEED LAFSTKEEQQ QLLQKVLAAT DLDAEEEVVA GEFGSRSSQA SRRFGTMSSM SGADDTVYME YHSSRSKAPS KHVHPLFKRF RK //