ID   AKIR2_HUMAN             Reviewed;         203 AA.
AC   Q53H80; Q9BQB1;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 2.
DT   10-FEB-2021, entry version 126.
DE   RecName: Full=Akirin-2;
GN   Name=AKIRIN2; Synonyms=C6orf166;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain;
RA   Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA   Tanaka A., Yokoyama S.;
RL   Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=14574404; DOI=10.1038/nature02055;
RA   Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA   Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA   Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA   Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA   Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA   Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA   Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA   Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA   Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA   Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA   French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA   Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA   Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA   Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA   Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA   Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA   Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA   Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA   Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA   Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA   Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA   Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA   Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA   Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA   Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA   Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA   West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA   Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA   Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA   Rogers J., Beck S.;
RT   "The DNA sequence and analysis of human chromosome 6.";
RL   Nature 425:805-811(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung carcinoma;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=17081983; DOI=10.1016/j.cell.2006.09.026;
RA   Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.;
RT   "Global, in vivo, and site-specific phosphorylation dynamics in signaling
RT   networks.";
RL   Cell 127:635-648(2006).
RN   [5]
RP   SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=18066067; DOI=10.1038/ni1543;
RA   Goto A., Matsushita K., Gesellchen V., El Chamy L., Kuttenkeuler D.,
RA   Takeuchi O., Hoffmann J.A., Akira S., Boutros M., Reichhart J.-M.;
RT   "Akirins are highly conserved nuclear proteins required for NF-kappaB-
RT   dependent gene expression in Drosophila and mice.";
RL   Nat. Immunol. 9:97-104(2008).
RN   [6]
RP   ERRATUM OF PUBMED:18066067.
RA   Goto A., Matsushita K., Gesellchen V., El Chamy L., Kuttenkeuler D.,
RA   Takeuchi O., Hoffmann J.A., Akira S., Boutros M., Reichhart J.-M.;
RL   Nat. Immunol. 9:216-216(2008).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18 AND SER-21, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA   Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA   Elledge S.J., Gygi S.P.;
RT   "A quantitative atlas of mitotic phosphorylation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Leukemic T-cell;
RX   PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA   Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA   Rodionov V., Han D.K.;
RT   "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT   reveals system-wide modulation of protein-protein interactions.";
RL   Sci. Signal. 2:RA46-RA46(2009).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18 AND SER-21, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA   Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA   Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT   "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT   site occupancy during mitosis.";
RL   Sci. Signal. 3:RA3-RA3(2010).
RN   [10]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18 AND SER-21, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma, and Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
CC   -!- FUNCTION: Required for the innate immune response. Downstream effector
CC       of the Toll-like receptor (TLR), TNF and IL-1 beta signaling pathways
CC       leading to the production of IL-6. Forms a complex with YWHAB that acts
CC       to repress transcription of DUSP1 (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with YWHAB. {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q53H80; Q53H80: AKIRIN2; NbExp=3; IntAct=EBI-742928, EBI-742928;
CC       Q53H80; P52597: HNRNPF; NbExp=3; IntAct=EBI-742928, EBI-352986;
CC       Q53H80; Q9BPX1: HSD17B14; NbExp=3; IntAct=EBI-742928, EBI-742664;
CC       Q53H80; Q14696: MESD; NbExp=3; IntAct=EBI-742928, EBI-6165891;
CC       Q53H80; Q8TDC0: MYOZ3; NbExp=3; IntAct=EBI-742928, EBI-5662487;
CC       Q53H80; Q96HA8: NTAQ1; NbExp=3; IntAct=EBI-742928, EBI-741158;
CC       Q53H80; O60504: SORBS3; NbExp=3; IntAct=EBI-742928, EBI-741237;
CC       Q53H80; Q9NZD8: SPG21; NbExp=11; IntAct=EBI-742928, EBI-742688;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18066067}.
CC   -!- TISSUE SPECIFICITY: Widely expressed with the highest expression in
CC       peripheral blood leukocytes. {ECO:0000269|PubMed:18066067}.
CC   -!- MISCELLANEOUS: 'Akiraka ni suru' means 'making things clear' in
CC       Japanese. The name is given based on the presence of the clear nuclear
CC       localization signal.
CC   -!- SIMILARITY: Belongs to the akirin family. {ECO:0000305}.
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DR   EMBL; AK222701; BAD96421.1; -; mRNA.
DR   EMBL; AL133211; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC000764; AAH00764.1; -; mRNA.
DR   EMBL; BC003042; AAH03042.1; -; mRNA.
DR   EMBL; BC005051; AAH05051.1; -; mRNA.
DR   CCDS; CCDS5013.1; -.
DR   RefSeq; NP_060534.1; NM_018064.3.
DR   BioGRID; 120430; 36.
DR   IntAct; Q53H80; 24.
DR   STRING; 9606.ENSP00000257787; -.
DR   iPTMnet; Q53H80; -.
DR   PhosphoSitePlus; Q53H80; -.
DR   BioMuta; AKIRIN2; -.
DR   DMDM; 71152385; -.
DR   EPD; Q53H80; -.
DR   jPOST; Q53H80; -.
DR   MassIVE; Q53H80; -.
DR   MaxQB; Q53H80; -.
DR   PaxDb; Q53H80; -.
DR   PeptideAtlas; Q53H80; -.
DR   PRIDE; Q53H80; -.
DR   ProteomicsDB; 62500; -.
DR   Antibodypedia; 31831; 179 antibodies.
DR   Ensembl; ENST00000257787; ENSP00000257787; ENSG00000135334.
DR   GeneID; 55122; -.
DR   KEGG; hsa:55122; -.
DR   UCSC; uc003pmk.4; human.
DR   CTD; 55122; -.
DR   DisGeNET; 55122; -.
DR   GeneCards; AKIRIN2; -.
DR   HGNC; HGNC:21407; AKIRIN2.
DR   HPA; ENSG00000135334; Low tissue specificity.
DR   MIM; 615165; gene.
DR   neXtProt; NX_Q53H80; -.
DR   OpenTargets; ENSG00000135334; -.
DR   PharmGKB; PA162376195; -.
DR   VEuPathDB; HostDB:ENSG00000135334.8; -.
DR   eggNOG; KOG4330; Eukaryota.
DR   GeneTree; ENSGT00940000156096; -.
DR   HOGENOM; CLU_119227_0_0_1; -.
DR   InParanoid; Q53H80; -.
DR   OMA; KMAENLC; -.
DR   OrthoDB; 1420469at2759; -.
DR   PhylomeDB; Q53H80; -.
DR   TreeFam; TF317123; -.
DR   PathwayCommons; Q53H80; -.
DR   BioGRID-ORCS; 55122; 683 hits in 882 CRISPR screens.
DR   ChiTaRS; AKIRIN2; human.
DR   GeneWiki; C6orf166; -.
DR   GenomeRNAi; 55122; -.
DR   Pharos; Q53H80; Tbio.
DR   PRO; PR:Q53H80; -.
DR   Proteomes; UP000005640; Chromosome 6.
DR   RNAct; Q53H80; protein.
DR   Bgee; ENSG00000135334; Expressed in oocyte and 214 other tissues.
DR   Genevisible; Q53H80; HS.
DR   GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR   GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR   GO; GO:0017053; C:transcription repressor complex; ISS:UniProtKB.
DR   GO; GO:0019899; F:enzyme binding; IPI:UniProtKB.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0009792; P:embryo development ending in birth or egg hatching; IEA:Ensembl.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; IEA:Ensembl.
DR   GO; GO:0010950; P:positive regulation of endopeptidase activity; IEA:Ensembl.
DR   GO; GO:0032755; P:positive regulation of interleukin-6 production; IEA:Ensembl.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR   GO; GO:0032496; P:response to lipopolysaccharide; IEA:Ensembl.
DR   InterPro; IPR024132; Akirin.
DR   PANTHER; PTHR13293; PTHR13293; 1.
PE   1: Evidence at protein level;
KW   Developmental protein; Immunity; Innate immunity; Nucleus; Phosphoprotein;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..203
FT                   /note="Akirin-2"
FT                   /id="PRO_0000089555"
FT   MOTIF           22..27
FT                   /note="Nuclear localization signal"
FT   COMPBIAS        28..56
FT                   /note="Ala-rich"
FT   MOD_RES         18
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000244|PubMed:18669648,
FT                   ECO:0000244|PubMed:20068231, ECO:0000244|PubMed:23186163"
FT   MOD_RES         21
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000244|PubMed:17081983,
FT                   ECO:0000244|PubMed:18669648, ECO:0000244|PubMed:19690332,
FT                   ECO:0000244|PubMed:20068231, ECO:0000244|PubMed:23186163"
FT   MOD_RES         57
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:B1AXD8"
FT   CONFLICT        171
FT                   /note="L -> S (in Ref. 1; BAD96421)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   203 AA;  22496 MW;  33AA93AF94DE80A1 CRC64;
     MACGATLKRT LDFDPLLSPA SPKRRRCAPL SAPTSAAASP LSAAAATAAS FSAAAASPQK
     YLRMEPSPFG DVSSRLTTEQ ILYNIKQEYK RMQKRRHLET SFQQTDPCCT SDAQPHAFLL
     SGPASPGTSS AASSPLKKEQ PLFTLRQVGM ICERLLKERE EKVREEYEEI LNTKLAEQYD
     AFVKFTHDQI MRRYGEQPAS YVS
//