ID   SL9B1_HUMAN             Reviewed;         515 AA.
AC   Q4ZJI4; A1KXV1; B9EH04; C9JBP7; Q49A30; Q8NCV2; Q8WVZ0;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 2.
DT   02-OCT-2024, entry version 149.
DE   RecName: Full=Sodium/hydrogen exchanger 9B1;
DE   AltName: Full=Na(+)/H(+) exchanger-like domain-containing protein 1;
DE            Short=NHE domain-containing protein 1;
DE   AltName: Full=Sodium/hydrogen exchanger-like domain-containing protein 1 {ECO:0000303|PubMed:16850186};
DE   AltName: Full=Solute carrier family 9, subfamily B member 1;
GN   Name=SLC9B1 {ECO:0000312|HGNC:HGNC:24244};
GN   Synonyms=NHA1 {ECO:0000312|HGNC:HGNC:24244},
GN   NHEDC1 {ECO:0000303|PubMed:16850186};
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND VARIANT
RP   HIS-33.
RC   TISSUE=Testis;
RX   PubMed=16850186; DOI=10.1007/s11033-006-0010-y;
RA   Ye G.M., Chen C., Han D., Xiong X., Kong Y., Wan B., Yu L.;
RT   "Cloning of a novel human NHEDC1 (Na+/H+ exchanger like domain containing
RT   1) gene expressed specifically in testis.";
RL   Mol. Biol. Rep. 33:175-180(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT HIS-33.
RC   TISSUE=Testis;
RA   Guo J.H., Yu L.;
RL   Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND VARIANT HIS-33.
RA   Li X., Xie Y., Mao Y.;
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA   Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA   Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA   Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA   Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA   Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA   Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA   Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA   Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA   Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA   Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA   Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA   Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA   Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA   Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA   Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA   Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT   4.";
RL   Nature 434:724-731(2005).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT HIS-33.
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 4), AND VARIANT
RP   HIS-33.
RC   TISSUE=Brain, and Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Sperm-specific Na(+)/H(+) exchanger involved in intracellular
CC       pH regulation of spermatozoa. Involved in sperm motility and fertility.
CC       {ECO:0000250|UniProtKB:Q8C0X2}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium, flagellum membrane
CC       {ECO:0000250|UniProtKB:Q8C0X2}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=1;
CC         IsoId=Q4ZJI4-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q4ZJI4-2; Sequence=VSP_030175, VSP_030180;
CC       Name=3;
CC         IsoId=Q4ZJI4-3; Sequence=VSP_030180;
CC       Name=4;
CC         IsoId=Q4ZJI4-5; Sequence=VSP_030176, VSP_030177;
CC   -!- TISSUE SPECIFICITY: Expressed only in the testis.
CC       {ECO:0000269|PubMed:16850186}.
CC   -!- MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a
CC       premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC       decay. {ECO:0000305}.
CC   -!- MISCELLANEOUS: [Isoform 4]: May be produced at very low levels due to a
CC       premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC       decay. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1)
CC       transporter (TC 2.A.36) family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM22868.1; Type=Frameshift; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DQ003059; AAY21808.1; -; mRNA.
DR   EMBL; AF447585; AAM22868.1; ALT_FRAME; mRNA.
DR   EMBL; AY461581; AAS91021.1; -; mRNA.
DR   EMBL; AC018797; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC083826; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471057; EAX06152.1; -; Genomic_DNA.
DR   EMBL; BC022079; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; BC046636; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; BC110794; AAI10795.1; -; mRNA.
DR   EMBL; BC132712; AAI32713.1; -; mRNA.
DR   EMBL; BC136966; AAI36967.1; -; mRNA.
DR   CCDS; CCDS34041.1; -. [Q4ZJI4-1]
DR   CCDS; CCDS47119.1; -. [Q4ZJI4-3]
DR   RefSeq; NP_001094344.1; NM_001100874.2. [Q4ZJI4-3]
DR   RefSeq; NP_631912.2; NM_139173.3. [Q4ZJI4-1]
DR   RefSeq; XP_006714156.1; XM_006714093.3.
DR   RefSeq; XP_011529924.1; XM_011531622.1.
DR   RefSeq; XP_011529925.1; XM_011531623.1.
DR   AlphaFoldDB; Q4ZJI4; -.
DR   SMR; Q4ZJI4; -.
DR   BioGRID; 127264; 1.
DR   IntAct; Q4ZJI4; 1.
DR   MINT; Q4ZJI4; -.
DR   STRING; 9606.ENSP00000296422; -.
DR   TCDB; 2.A.36.2.3; the monovalent cation:proton antiporter-1 (cpa1) family.
DR   GlyGen; Q4ZJI4; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; Q4ZJI4; -.
DR   PhosphoSitePlus; Q4ZJI4; -.
DR   BioMuta; SLC9B1; -.
DR   DMDM; 296439240; -.
DR   MassIVE; Q4ZJI4; -.
DR   PaxDb; 9606-ENSP00000296422; -.
DR   PeptideAtlas; Q4ZJI4; -.
DR   ProteomicsDB; 62386; -. [Q4ZJI4-1]
DR   ProteomicsDB; 62387; -. [Q4ZJI4-2]
DR   ProteomicsDB; 62388; -. [Q4ZJI4-3]
DR   ProteomicsDB; 62389; -. [Q4ZJI4-5]
DR   Antibodypedia; 26068; 101 antibodies from 20 providers.
DR   DNASU; 150159; -.
DR   Ensembl; ENST00000296422.12; ENSP00000296422.7; ENSG00000164037.17. [Q4ZJI4-1]
DR   Ensembl; ENST00000394789.7; ENSP00000378269.3; ENSG00000164037.17. [Q4ZJI4-3]
DR   Ensembl; ENST00000503584.5; ENSP00000426926.1; ENSG00000164037.17. [Q4ZJI4-5]
DR   Ensembl; ENST00000510243.5; ENSP00000425913.1; ENSG00000164037.17. [Q4ZJI4-5]
DR   GeneID; 150159; -.
DR   KEGG; hsa:150159; -.
DR   MANE-Select; ENST00000296422.12; ENSP00000296422.7; NM_139173.4; NP_631912.3.
DR   UCSC; uc003hwu.4; human. [Q4ZJI4-1]
DR   AGR; HGNC:24244; -.
DR   CTD; 150159; -.
DR   DisGeNET; 150159; -.
DR   GeneCards; SLC9B1; -.
DR   HGNC; HGNC:24244; SLC9B1.
DR   HPA; ENSG00000164037; Tissue enriched (testis).
DR   MalaCards; SLC9B1; -.
DR   MIM; 611527; gene.
DR   neXtProt; NX_Q4ZJI4; -.
DR   OpenTargets; ENSG00000164037; -.
DR   PharmGKB; PA162397486; -.
DR   VEuPathDB; HostDB:ENSG00000164037; -.
DR   eggNOG; KOG3826; Eukaryota.
DR   GeneTree; ENSGT00390000013285; -.
DR   HOGENOM; CLU_201317_0_0_1; -.
DR   InParanoid; Q4ZJI4; -.
DR   OMA; ICVATMS; -.
DR   OrthoDB; 3228646at2759; -.
DR   PhylomeDB; Q4ZJI4; -.
DR   TreeFam; TF319087; -.
DR   PathwayCommons; Q4ZJI4; -.
DR   Reactome; R-HSA-2672351; Stimuli-sensing channels.
DR   BioGRID-ORCS; 150159; 372 hits in 1059 CRISPR screens.
DR   ChiTaRS; SLC9B1; human.
DR   GenomeRNAi; 150159; -.
DR   Pharos; Q4ZJI4; Tbio.
DR   PRO; PR:Q4ZJI4; -.
DR   Proteomes; UP000005640; Chromosome 4.
DR   RNAct; Q4ZJI4; protein.
DR   Bgee; ENSG00000164037; Expressed in sperm and 96 other cell types or tissues.
DR   ExpressionAtlas; Q4ZJI4; baseline and differential.
DR   GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR   GO; GO:0097228; C:sperm principal piece; ISS:UniProtKB.
DR   GO; GO:0015385; F:sodium:proton antiporter activity; TAS:Reactome.
DR   GO; GO:0030317; P:flagellated sperm motility; ISS:UniProtKB.
DR   GO; GO:0098662; P:inorganic cation transmembrane transport; IBA:GO_Central.
DR   GO; GO:0034220; P:monoatomic ion transmembrane transport; TAS:Reactome.
DR   GO; GO:0051453; P:regulation of intracellular pH; ISS:UniProtKB.
DR   GO; GO:0007338; P:single fertilization; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.1530.20; -; 1.
DR   InterPro; IPR006153; Cation/H_exchanger.
DR   InterPro; IPR051843; CPA1_transporter.
DR   InterPro; IPR038770; Na+/solute_symporter_sf.
DR   PANTHER; PTHR31102; -; 1.
DR   PANTHER; PTHR31102:SF5; SLC9B1-LIKE PROTEIN SLC9B1P1-RELATED; 1.
DR   Pfam; PF00999; Na_H_Exchanger; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Antiport; Cell membrane; Cell projection; Cilium;
KW   Fertilization; Flagellum; Ion transport; Membrane;
KW   Proteomics identification; Reference proteome; Sodium; Sodium transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..515
FT                   /note="Sodium/hydrogen exchanger 9B1"
FT                   /id="PRO_0000314007"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..136
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        152..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        187..207
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        215..235
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        260..280
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..304
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        337..357
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        368..388
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        407..427
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        431..451
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        472..492
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..32
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        15..32
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VAR_SEQ         1..232
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_030175"
FT   VAR_SEQ         71..73
FT                   /note="GVI -> VLF (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_030176"
FT   VAR_SEQ         74..515
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:15489334"
FT                   /id="VSP_030177"
FT   VAR_SEQ         445..515
FT                   /note="AVLGPLALETARVSAPHLEPYAKDVMTVAFLAILITAPNGALLMGILGPKML
FT                   TRHYDPSKIKLQLSTLEHH -> INQAILLLFLLREEWTNCKVAKKCEYTKERQ (in
FT                   isoform 2 and isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.3"
FT                   /id="VSP_030180"
FT   VARIANT         33
FT                   /note="Q -> H (in dbSNP:rs2715591)"
FT                   /evidence="ECO:0000269|PubMed:15489334,
FT                   ECO:0000269|PubMed:16850186, ECO:0000269|Ref.2,
FT                   ECO:0000269|Ref.3, ECO:0000269|Ref.5"
FT                   /id="VAR_061370"
FT   CONFLICT        292
FT                   /note="I -> T (in Ref. 3; AAS91021)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   515 AA;  56054 MW;  37E89E22A8A910E5 CRC64;
     MHTTESKNEH LEDENFQTST TPQSLIDPNN TAQEETKTVL SDTEEIKPQT KKETYISCPL
     RGVLNVIITN GVILFVIWCM TWSILGSEAL PGGNLFGLFI IFYSAIIGGK ILQLIRIPLV
     PPLPPLLGML LAGFTIRNVP FINEHVHVPN TWSSILRSIA LTIILIRAGL GLDPQALRHL
     KVVCFRLAVG PCLMEASAAA VFSHFIMKFP WQWAFLLGFV LGAVSPAVVV PYMMVLQENG
     YGVEEGIPTL LMAASSMDDI LAITGFNTCL SIVFSSGGIL NNAIASIRNV CISLLAGIVL
     GFFVRYFPSE DQKKLTLKRG FLVLTMCVSA VLGSQRIGLH GSGGLCTLVL SFIAGTKWSQ
     EKMKVQKIIT TVWDIFQPLL FGLVGAEVSV SSLESNIVGI SVATLSLALC VRILTTYLLM
     CFAGFSFKEK IFIALAWMPK ATVQAVLGPL ALETARVSAP HLEPYAKDVM TVAFLAILIT
     APNGALLMGI LGPKMLTRHY DPSKIKLQLS TLEHH
//