ID HAS1_ASPFU Reviewed; 622 AA. AC Q4WQM4; DT 04-APR-2006, integrated into UniProtKB/Swiss-Prot. DT 04-APR-2006, sequence version 2. DT 26-FEB-2020, entry version 95. DE RecName: Full=ATP-dependent RNA helicase has1; DE EC=3.6.4.13; GN Name=has1; ORFNames=AFUA_4G13330; OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC OS A1100) (Aspergillus fumigatus). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus. OX NCBI_TaxID=330879; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100; RX PubMed=16372009; DOI=10.1038/nature04332; RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J., RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P., RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L., RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N., RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K., RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E., RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H., RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A., RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L., RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D., RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L., RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U., RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M., RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C., RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F., RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R., RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N., RA Barrell B.G., Denning D.W.; RT "Genomic sequence of the pathogenic and allergenic filamentous fungus RT Aspergillus fumigatus."; RL Nature 438:1151-1156(2005). CC -!- FUNCTION: ATP-dependent RNA helicase involved in 40S ribosomal subunit CC biogenesis. Required for the processing and cleavage of 35S pre-rRNA at CC sites A0, A1, and A2, leading to mature 18S rRNA. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; CC -!- SUBUNIT: Associates in the nucleolus with the 60S and pre-60S ribosomal CC subunits. {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}. CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box CC family of RNA helicases and controls ATP binding and hydrolysis. CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX18/HAS1 CC subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AAHF01000005; EAL89460.2; -; Genomic_DNA. DR RefSeq; XP_751498.2; XM_746405.2. DR SMR; Q4WQM4; -. DR STRING; 746128.CADAFUBP00006842; -. DR EnsemblFungi; EAL89460; EAL89460; AFUA_4G13330. DR GeneID; 3509518; -. DR KEGG; afm:AFUA_4G13330; -. DR EuPathDB; FungiDB:Afu4g13330; -. DR HOGENOM; CLU_003041_26_5_1; -. DR InParanoid; Q4WQM4; -. DR KO; K13179; -. DR OMA; AHHSQTY; -. DR OrthoDB; 973872at2759; -. DR Proteomes; UP000002530; Chromosome 4. DR Proteomes; UP000002530; Unassembled WGS sequence. DR GO; GO:0005730; C:nucleolus; IBA:GO_Central. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0003723; F:RNA binding; IBA:GO_Central. DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central. DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central. DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom. DR InterPro; IPR025313; DUF4217. DR InterPro; IPR014001; Helicase_ATP-bd. DR InterPro; IPR001650; Helicase_C. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS. DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif. DR Pfam; PF00270; DEAD; 1. DR Pfam; PF13959; DUF4217; 1. DR Pfam; PF00271; Helicase_C; 1. DR SMART; SM00487; DEXDc; 1. DR SMART; SM01178; DUF4217; 1. DR SMART; SM00490; HELICc; 1. DR SUPFAM; SSF52540; SSF52540; 2. DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1. DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1. DR PROSITE; PS51194; HELICASE_CTER; 1. DR PROSITE; PS51195; Q_MOTIF; 1. PE 3: Inferred from homology; KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; KW Reference proteome; Ribosome biogenesis; RNA-binding; rRNA processing. FT CHAIN 1..622 FT /note="ATP-dependent RNA helicase has1" FT /id="PRO_0000232205" FT DOMAIN 171..347 FT /note="Helicase ATP-binding" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541" FT DOMAIN 361..531 FT /note="Helicase C-terminal" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542" FT NP_BIND 184..191 FT /note="ATP" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541" FT MOTIF 140..168 FT /note="Q motif" FT MOTIF 294..297 FT /note="DEAD box" FT MOTIF 373..389 FT /note="Bipartite nuclear localization signal" FT /evidence="ECO:0000250" SQ SEQUENCE 622 AA; 69202 MW; 4C2226E7F937CE74 CRC64; MSGPVDTAKS ITKKRKRKHG GGARAATETD DAITRPAIEN GAVNDSPEKE EDTKKSEKNG KDKSAKKRKV SHASSDEGDE SQEEQGAPAQ ADGDSDDNKD DGNDQSEAEN GDNGDKKDTE STDLPSAGTL SLPTVEGEPQ KFTELGLSEK TLKAINDMGF ETMTEIQRRT IPPLLAGRDV LGAAKTGSGK TLSFLIPAVE MLSALRFKPR NGTGVIVVSP TRELALQIFG VARELCQYHS QTYGIVIGGA NRRAEAEKLM KGVNLLIATP GRLLDHLQNT QGFVFKNLKT LVIDEADRIL EVGFEDEMRQ IVKILPSEER QTMLFSATQT TKVEDLARIS LRPGPLYINV DHRKEHSTVE GLEQGYVICE ADKRFLLLFS FLKRNLKKKI IVFFSSCNCV KYHAELLNYI DLPVLELHGK QKQQKRTNTF FEFCNAKQGT LICTDVAARG LDIPAVDWII QFDPPDDPRD YIHRVGRTAR GTNAKGRSLM FLQPSEVGFL KHLKEARVPV VEFEFPANKI VNVQSQLEKL IGQNYYLNKS AKEGYRSYLQ AYASHSLRSV FDVHKLDLVK VAKGFGFSTP PRIDIQLGAS LSRDKKQQQQ GRRSYGSQPH SKGLKFKRKH DD //