ID KDUI_ECOLI Reviewed; 278 AA. AC Q46938; Q2M9Z4; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 25-JAN-2012, entry version 87. DE RecName: Full=4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase; DE EC=5.3.1.17; DE AltName: Full=5-keto-4-deoxyuronate isomerase; DE AltName: Full=DKI isomerase; GN Name=kduI; Synonyms=yqeE; OrderedLocusNames=b2843, JW2811; OS Escherichia coli (strain K12). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=83333; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., RA Mau B., Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1474(1997). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=16738553; DOI=10.1038/msb4100049; RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; RT "Highly accurate genome sequences of Escherichia coli K-12 strains RT MG1655 and W3110."; RL Mol. Syst. Biol. 2:E1-E5(2006). RN [3] RP CRYSTALLIZATION, AND PROTEIN SEQUENCE OF 1-20 AND 227-247. RX MEDLINE=98437591; PubMed=9761873; DOI=10.1107/S090744499701785X; RA Dunten P., Jaffe H., Aksamit R.R.; RT "Crystallization of 5-keto-4-deoxyuronate isomerase from Escherichia RT coli."; RL Acta Crystallogr. D 54:678-680(1998). CC -!- CATALYTIC ACTIVITY: 4-deoxy-L-threo-5-hexosulose uronate = 3- CC deoxy-D-glycero-2,5-hexodiulosonate. CC -!- PATHWAY: Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy- CC D-gluconate from pectin: step 4/5. CC -!- SUBUNIT: Homohexamer. CC -!- SIMILARITY: Belongs to the kduI family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U29581; AAB40490.1; -; Genomic_DNA. DR EMBL; U00096; AAC75882.1; -; Genomic_DNA. DR EMBL; AP009048; BAE76912.1; -; Genomic_DNA. DR PIR; D65067; D65067. DR RefSeq; NP_417320.1; NC_000913.2. DR PDB; 1X8M; X-ray; 2.60 A; A/B/C/D/E/F=2-278. DR PDB; 1XRU; X-ray; 1.94 A; A/B=1-275. DR PDBsum; 1X8M; -. DR PDBsum; 1XRU; -. DR ProteinModelPortal; Q46938; -. DR SMR; Q46938; 1-277. DR DIP; DIP-10069N; -. DR IntAct; Q46938; 4. DR EnsemblBacteria; EBESCT00000002790; EBESCP00000002790; EBESCG00000002276. DR EnsemblBacteria; EBESCT00000014597; EBESCP00000013888; EBESCG00000013658. DR GeneID; 947319; -. DR GenomeReviews; AP009048_GR; JW2811. DR GenomeReviews; U00096_GR; b2843. DR KEGG; ecj:JW2811; -. DR KEGG; eco:b2843; -. DR PATRIC; 32121104; VBIEscCol129921_2941. DR EchoBASE; EB2899; -. DR EcoGene; EG13096; kduI. DR eggNOG; COG3717; -. DR GeneTree; EBGT00050000011662; -. DR HOGENOM; HBG301328; -. DR OMA; AKFYLVS; -. DR PhylomeDB; Q46938; -. DR ProtClustDB; PRK00924; -. DR BioCyc; EcoCyc:G7463-MONOMER; -. DR BRENDA; 5.3.1.17; 2026. DR Genevestigator; Q46938; -. DR GO; GO:0005829; C:cytosol; IDA:UniProtKB. DR GO; GO:0008697; F:4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase activity; IEA:EC. DR GO; GO:0045490; P:pectin catabolic process; IEA:InterPro. DR HAMAP; MF_00687; KduI; 1; -. DR InterPro; IPR011051; Cupin_RmlC_type. DR InterPro; IPR007045; KduI. DR InterPro; IPR021120; KduI/IolB_isomerase. DR KO; K01815; -. DR Pfam; PF04962; KduI; 1. DR PIRSF; PIRSF006625; KduI; 1. DR SUPFAM; SSF51182; RmlC_like_cupin; 1. PE 1: Evidence at protein level; KW 3D-structure; Complete proteome; Direct protein sequencing; Isomerase; KW Reference proteome. FT CHAIN 1 278 4-deoxy-L-threo-5-hexosulose-uronate FT ketol-isomerase. FT /FTId=PRO_0000215485. FT STRAND 2 4 FT HELIX 9 12 FT HELIX 17 24 FT STRAND 38 40 FT TURN 41 44 FT STRAND 45 51 FT STRAND 53 55 FT STRAND 57 60 FT HELIX 61 66 FT STRAND 69 71 FT TURN 72 75 FT STRAND 76 82 FT STRAND 87 91 FT STRAND 94 98 FT STRAND 103 106 FT STRAND 113 118 FT STRAND 126 132 FT STRAND 140 142 FT HELIX 144 147 FT STRAND 150 152 FT HELIX 155 157 FT STRAND 161 168 FT TURN 169 171 FT STRAND 178 184 FT STRAND 190 192 FT STRAND 194 197 FT STRAND 201 209 FT STRAND 216 222 FT STRAND 225 231 FT STRAND 233 239 FT STRAND 244 251 FT STRAND 254 262 FT STRAND 269 272 SQ SEQUENCE 278 AA; 31076 MW; F7CD5C259503CD1A CRC64; MDVRQSIHSA HAKTLDTQGL RNEFLVEKVF VADEYTMVYS HIDRIIVGGI MPITKTVSVG GEVGKQLGVS YFLERRELGV INIGGAGTIT VDGQCYEIGH RDALYVGKGA KEVVFASIDT GTPAKFYYNC APAHTTYPTK KVTPDEVSPV TLGDNLTSNR RTINKYFVPD VLETCQLSMG LTELAPGNLW NTMPCHTHER RMEVYFYFNM DDDACVFHMM GQPQETRHIV MHNEQAVISP SWSIHSGVGT KAYTFIWGMV GENQVFDDMD HVAVKDLR //