ID KDUI_ECOLI STANDARD; PRT; 278 AA. AC Q46938; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 07-FEB-2006, entry version 35. DE 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC 5.3.1.17) (5- DE keto-4-deoxyuronate isomerase) (DKI isomerase). GN Name=kduI; OrderedLocusNames=b2843; OS Escherichia coli. OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=562; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655; RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., RA Mau B., Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1474(1997). RN [2] RP CRYSTALLIZATION, AND PROTEIN SEQUENCE OF 1-20 AND 227-247. RX MEDLINE=98437591; PubMed=9761873; DOI=10.1107/S090744499701785X; RA Dunten P., Jaffe H., Aksamit R.R.; RT "Crystallization of 5-keto-4-deoxyuronate isomerase from Escherichia RT coli."; RL Acta Crystallogr. D 54:678-680(1998). CC -!- CATALYTIC ACTIVITY: 4-deoxy-L-threo-5-hexosulose uronate = 3- CC deoxy-D-glycero-2,5-hexodiulosonate. CC -!- PATHWAY: Pectin degradation. CC -!- SUBUNIT: Homohexamer. CC -!- SIMILARITY: Belongs to the kduI family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U29581; AAB40490.1; -; Genomic_DNA. DR EMBL; U00096; AAC75882.1; -; Genomic_DNA. DR PIR; D65067; D65067. DR PDB; 1X8M; X-ray; A/B/C/D/E/F=2-278. DR EchoBASE; EB2899; -. DR EcoGene; EG13096; kduI. DR BioCyc; EcoCyc:G7463-MONOMER; -. DR HAMAP; MF_00687; -; 1. DR InterPro; IPR007045; KduI. DR Pfam; PF04962; KduI; 1. DR PIRSF; PIRSF006625; KduI; 1. KW 3D-structure; Complete proteome; Direct protein sequencing; Isomerase. FT CHAIN 1 278 4-deoxy-L-threo-5-hexosulose-uronate FT ketol-isomerase. FT /FTId=PRO_0000215485. FT STRAND 2 4 FT HELIX 9 13 FT TURN 14 14 FT HELIX 17 24 FT STRAND 25 26 FT STRAND 32 32 FT TURN 33 33 FT STRAND 34 35 FT STRAND 38 40 FT TURN 41 44 FT STRAND 45 51 FT STRAND 53 55 FT STRAND 57 60 FT HELIX 61 67 FT TURN 68 68 FT STRAND 69 71 FT HELIX 72 75 FT STRAND 76 82 FT STRAND 84 91 FT TURN 92 93 FT STRAND 94 98 FT TURN 100 101 FT STRAND 103 106 FT TURN 108 109 FT STRAND 113 118 FT TURN 120 121 FT STRAND 122 122 FT STRAND 126 132 FT STRAND 134 135 FT STRAND 140 142 FT TURN 144 146 FT STRAND 147 147 FT STRAND 150 152 FT TURN 155 158 FT STRAND 161 168 FT TURN 169 171 FT STRAND 175 176 FT STRAND 178 184 FT TURN 186 187 FT STRAND 190 190 FT STRAND 202 209 FT STRAND 212 213 FT STRAND 216 222 FT TURN 223 224 FT STRAND 225 225 FT STRAND 227 231 FT STRAND 233 239 FT TURN 241 242 FT STRAND 243 243 FT STRAND 247 251 FT STRAND 254 263 SQ SEQUENCE 278 AA; 31076 MW; F7CD5C259503CD1A CRC64; MDVRQSIHSA HAKTLDTQGL RNEFLVEKVF VADEYTMVYS HIDRIIVGGI MPITKTVSVG GEVGKQLGVS YFLERRELGV INIGGAGTIT VDGQCYEIGH RDALYVGKGA KEVVFASIDT GTPAKFYYNC APAHTTYPTK KVTPDEVSPV TLGDNLTSNR RTINKYFVPD VLETCQLSMG LTELAPGNLW NTMPCHTHER RMEVYFYFNM DDDACVFHMM GQPQETRHIV MHNEQAVISP SWSIHSGVGT KAYTFIWGMV GENQVFDDMD HVAVKDLR //