ID TOC34_PEA Reviewed; 310 AA. AC Q41009; Q41029; DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 16-APR-2014, entry version 90. DE RecName: Full=Translocase of chloroplast 34; DE EC=3.6.5.-; DE AltName: Full=34 kDa chloroplast outer envelope protein; DE AltName: Full=GTP-binding protein IAP34; DE AltName: Full=GTP-binding protein OEP34; GN Name=TOC34; Synonyms=IAP34, OEP34; OS Pisum sativum (Garden pea). OG Plastid; Chloroplast. OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; OC Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; OC Fabeae; Pisum. OX NCBI_TaxID=3888; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE. RC STRAIN=cv. Miranda; TISSUE=Leaf; RX PubMed=7757113; DOI=10.1046/j.1365-313X.1995.7030401.x; RA Seedorf M., Waegemann K., Soll J.; RT "A constituent of the chloroplast import complex represents a new type RT of GTP-binding protein."; RL Plant J. 7:401-411(1995). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 119-139 AND RP 157-180. RX PubMed=7973656; DOI=10.1126/science.7973656; RA Kessler F., Blobel G., Patel H.V., Schnell D.J.; RT "Identification of two GTP-binding proteins in the chloroplast protein RT import machinery."; RL Science 266:1035-1039(1994). RN [3] RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-258 IN COMPLEX WITH GTP, RP HOMODIMERIZATION, AND MUTAGENESIS OF ARG-128. RX PubMed=11753431; DOI=10.1038/nsb744; RA Sun Y.-J., Forouhar F., Li Hm H.-M., Tu S.-L., Yeh Y.-H., Kao S., RA Shr H.-L., Chou C.-C., Chen C., Hsiao C.-D.; RT "Crystal structure of pea Toc34, a novel GTPase of the chloroplast RT protein translocon."; RL Nat. Struct. Biol. 9:95-100(2002). CC -!- FUNCTION: GTPase involved in protein precursor import into CC chloroplasts. Seems to recognize chloroplast-destined precursor CC proteins and regulate their presentation to the translocation CC channel through GTP hydrolysis. CC -!- SUBUNIT: Homodimer and monomer. Part of the TOC core complex that CC includes a protein for the specific recognition of transit CC peptides surrounded by a ring composed of four proteins forming CC translocation channels, and four to five GTP-binding proteins CC providing energy. This core complex can interact with components CC of the TIC complex to form a larger import complex. Chloroplastic CC protein precursor such as prSS (precursor of the RuBisCO small CC subunit) interacts with these complexes. The TOC complex contains CC a specific subset of polar lipids such as CC digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and CC phosphatidylglycerol (PG). TOC34 interacts at least with TOC75. CC -!- INTERACTION: CC P48347:GRF10 (xeno); NbExp=2; IntAct=EBI-638506, EBI-1803304; CC Q9MUK5:TOC64; NbExp=6; IntAct=EBI-638506, EBI-638487; CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane. CC -!- SIMILARITY: Belongs to the TOC34 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z28341; CAA82196.1; -; mRNA. DR EMBL; L36856; AAC25785.1; -; mRNA. DR PIR; B55171; B55171. DR PDB; 1H65; X-ray; 2.00 A; A/B/C=1-258. DR PDB; 3BB1; X-ray; 2.80 A; A/B/C/D/E/F/G/H=1-266. DR PDBsum; 1H65; -. DR PDBsum; 3BB1; -. DR ProteinModelPortal; Q41009; -. DR SMR; Q41009; 7-258. DR DIP; DIP-332N; -. DR IntAct; Q41009; 9. DR MINT; MINT-2584005; -. DR TCDB; 3.A.9.1.1; the chloroplast envelope protein translocase (cept or tic-toc) family. DR EvolutionaryTrace; Q41009; -. DR GO; GO:0009707; C:chloroplast outer membrane; IEA:UniProtKB-SubCell. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW. DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW. DR GO; GO:0015450; F:P-P-bond-hydrolysis-driven protein transmembrane transporter activity; IEA:InterPro. DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro. DR Gene3D; 3.40.50.300; -; 1. DR InterPro; IPR006703; AIG1. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR005688; Toc34. DR Pfam; PF04548; AIG1; 1. DR PIRSF; PIRSF038134; Toc34; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR TIGRFAMs; TIGR00991; 3a0901s02IAP34; 1. PE 1: Evidence at protein level; KW 3D-structure; Chloroplast; Direct protein sequencing; GTP-binding; KW Hydrolase; Membrane; Nucleotide-binding; Plastid; KW Plastid outer membrane; Protein transport; Transmembrane; KW Transmembrane helix; Transport. FT CHAIN 1 310 Translocase of chloroplast 34. FT /FTId=PRO_0000144793. FT TOPO_DOM 1 268 Cytoplasmic (Potential). FT TRANSMEM 269 289 Helical; (Potential). FT TOPO_DOM 290 310 Chloroplast intermembrane (Potential). FT NP_BIND 49 54 GTP. FT NP_BIND 210 211 GTP. FT REGION 68 71 Homodimerization. FT REGION 128 133 Homodimerization. FT BINDING 73 73 GTP. FT MUTAGEN 128 128 R->A: No dimerization and reduced GTPase FT activity. FT HELIX 12 16 FT HELIX 19 34 FT STRAND 39 47 FT HELIX 52 60 FT STRAND 69 71 FT STRAND 78 84 FT STRAND 87 93 FT STRAND 97 99 FT HELIX 105 114 FT TURN 115 117 FT STRAND 122 130 FT HELIX 136 149 FT HELIX 151 156 FT STRAND 157 162 FT HELIX 169 171 FT HELIX 174 192 FT HELIX 200 202 FT STRAND 206 208 FT HELIX 231 243 FT STRAND 245 247 FT HELIX 254 258 SQ SEQUENCE 310 AA; 34157 MW; 00D5E79735BF041F CRC64; MASQQQTVRE WSGINTFAPA TQTKLLELLG NLKQEDVNSL TILVMGKGGV GKSSTVNSII GERVVSISPF QSEGPRPVMV SRSRAGFTLN IIDTPGLIEG GYINDMALNI IKSFLLDKTI DVLLYVDRLD AYRVDNLDKL VAKAITDSFG KGIWNKAIVA LTHAQFSPPD GLPYDEFFSK RSEALLQVVR SGASLKKDAQ ASDIPVVLIE NSGRCNKNDS DEKVLPNGIA WIPHLVQTIT EVALNKSESI FVDKNLIDGP NPNQRGKLWI PLIFALQYLF LAKPIEALIR RDIATETKPA WETRDVGDRK //