ID PRS44_MOUSE Reviewed; 372 AA. AC Q402U7; Q924U6; DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot. DT 27-SEP-2005, sequence version 1. DT 08-MAY-2019, entry version 100. DE RecName: Full=Serine protease 44 {ECO:0000305}; DE EC=3.4.21.-; DE AltName: Full=Testis serine protease 4; DE Short=TESSP-4; DE AltName: Full=Testis-specific serine protease 4; DE Flags: Precursor; GN Name=Prss44 {ECO:0000312|MGI:MGI:1920586}; GN Synonyms=Tessp4 {ECO:0000303|PubMed:23536369}; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; OC Muroidea; Muridae; Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). RX PubMed=19798924; DOI=10.2108/zsj.26.294; RA Takano N., Kimura A., Takahashi T.; RT "Two distinct localization patterns of testis-specific serine protease RT 1 (TESSP1) in the seminiferous tubules of the mouse testis."; RL Zool. Sci. 26:294-300(2009). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE. RX PubMed=23536369; DOI=10.1095/biolreprod.112.106328; RA Yoneda R., Takahashi T., Matsui H., Takano N., Hasebe Y., Ogiwara K., RA Kimura A.P.; RT "Three testis-specific paralogous serine proteases play different RT roles in murine spermatogenesis and are involved in germ cell survival RT during meiosis."; RL Biol. Reprod. 88:118-118(2013). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C57BL/6J; RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112; RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., RA She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., RA Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., RA Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., RA Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., RA Lindblad-Toh K., Eichler E.E., Ponting C.P.; RT "Lineage-specific biology revealed by a finished genome assembly of RT the mouse."; RL PLoS Biol. 7:E1000112-E1000112(2009). CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane CC protein {ECO:0000305}. Cytoplasm {ECO:0000269|PubMed:23536369}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; CC IsoId=Q402U7-1; Sequence=Displayed; CC Name=2; CC IsoId=Q402U7-2; Sequence=VSP_041974, VSP_041975; CC -!- TISSUE SPECIFICITY: Testis-specific (PubMed:23536369). Expressed CC by primary and secondary spermatocytes (PubMed:23536369). CC {ECO:0000269|PubMed:23536369}. CC -!- DEVELOPMENTAL STAGE: In testis, expressed at all stages from the CC late pachytene primary spermatocyte to the secondary spermatocyte. CC Not detected at day 7 after birth. Expression is detected at day CC 14 and increases dramatically at day 21 and reach a peak at day 28 CC to remain high until day 56. {ECO:0000269|PubMed:23536369}. CC -!- SIMILARITY: Belongs to the peptidase S1 family. CC {ECO:0000255|PROSITE-ProRule:PRU00274}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB162857; BAE19954.1; -; mRNA. DR EMBL; AB047758; BAB63919.1; -; mRNA. DR EMBL; AC139378; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR CCDS; CCDS40782.1; -. [Q402U7-1] DR RefSeq; NP_683742.2; NM_148940.3. [Q402U7-1] DR SMR; Q402U7; -. DR MEROPS; S01.106; -. DR PaxDb; Q402U7; -. DR PRIDE; Q402U7; -. DR DNASU; 73336; -. DR Ensembl; ENSMUST00000098345; ENSMUSP00000095948; ENSMUSG00000032493. [Q402U7-1] DR GeneID; 73336; -. DR KEGG; mmu:73336; -. DR UCSC; uc009rut.2; mouse. [Q402U7-1] DR UCSC; uc009ruu.2; mouse. [Q402U7-2] DR CTD; 73336; -. DR MGI; MGI:1920586; Prss44. DR eggNOG; KOG3627; Eukaryota. DR eggNOG; COG5640; LUCA. DR GeneTree; ENSGT00940000162829; -. DR HOGENOM; HOG000251820; -. DR InParanoid; Q402U7; -. DR OMA; IVIHRYF; -. DR OrthoDB; 1314811at2759; -. DR PhylomeDB; Q402U7; -. DR TreeFam; TF351676; -. DR PRO; PR:Q402U7; -. DR Proteomes; UP000000589; Chromosome 9. DR Bgee; ENSMUSG00000032493; Expressed in 115 organ(s), highest expression level in skeletal muscle tissue. DR ExpressionAtlas; Q402U7; baseline and differential. DR Genevisible; Q402U7; MM. DR GO; GO:0005737; C:cytoplasm; IDA:MGI. DR GO; GO:0005615; C:extracellular space; IBA:GO_Central. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0004252; F:serine-type endopeptidase activity; IBA:GO_Central. DR GO; GO:0007281; P:germ cell development; IBA:GO_Central. DR GO; GO:0006508; P:proteolysis; IBA:GO_Central. DR GO; GO:0007283; P:spermatogenesis; IBA:GO_Central. DR CDD; cd00190; Tryp_SPc; 1. DR InterPro; IPR009003; Peptidase_S1_PA. DR InterPro; IPR001314; Peptidase_S1A. DR InterPro; IPR001254; Trypsin_dom. DR InterPro; IPR018114; TRYPSIN_HIS. DR InterPro; IPR033116; TRYPSIN_SER. DR Pfam; PF00089; Trypsin; 1. DR PRINTS; PR00722; CHYMOTRYPSIN. DR SMART; SM00020; Tryp_SPc; 1. DR SUPFAM; SSF50494; SSF50494; 1. DR PROSITE; PS50240; TRYPSIN_DOM; 1. DR PROSITE; PS00134; TRYPSIN_HIS; 1. DR PROSITE; PS00135; TRYPSIN_SER; 1. PE 2: Evidence at transcript level; KW Alternative splicing; Complete proteome; Cytoplasm; Disulfide bond; KW Glycoprotein; Hydrolase; Membrane; Protease; Reference proteome; KW Serine protease; Signal; Transmembrane; Transmembrane helix. FT SIGNAL 1 25 {ECO:0000255}. FT CHAIN 26 372 Serine protease 44. FT /FTId=PRO_0000413699. FT TOPO_DOM 26 351 Extracellular. {ECO:0000255}. FT TRANSMEM 352 372 Helical. {ECO:0000255}. FT DOMAIN 112 345 Peptidase S1. {ECO:0000255|PROSITE- FT ProRule:PRU00274}. FT ACT_SITE 152 152 Charge relay system. {ECO:0000250}. FT ACT_SITE 197 197 Charge relay system. {ECO:0000250}. FT ACT_SITE 297 297 Charge relay system. {ECO:0000250}. FT CARBOHYD 208 208 N-linked (GlcNAc...) asparagine. FT {ECO:0000255}. FT DISULFID 137 153 {ECO:0000255|PROSITE-ProRule:PRU00274}. FT DISULFID 231 303 {ECO:0000255|PROSITE-ProRule:PRU00274}. FT DISULFID 262 283 {ECO:0000255|PROSITE-ProRule:PRU00274}. FT DISULFID 293 321 {ECO:0000255|PROSITE-ProRule:PRU00274}. FT VAR_SEQ 1 163 Missing (in isoform 2). FT {ECO:0000303|PubMed:23536369}. FT /FTId=VSP_041974. FT VAR_SEQ 334 372 SYYRDWIIKELSRASCWKLSGFLVLSVCLVLHLAIVVAL FT -> ASPNDTALPPTHRAHCPQDCFQDPRLAKME (in FT isoform 2). FT {ECO:0000303|PubMed:23536369}. FT /FTId=VSP_041975. SQ SEQUENCE 372 AA; 41073 MW; E15BB243F3B9784A CRC64; MAFQGCDCFG LLVWLLLLQT RLGKARMVPG TPSLSPLPSE NGLDDSGVNP QERPLTGMPE TSLPRKPGDS TRPLDSMAFT PGQSFSTMSL SRQPFPTWVP PTSACGHRTA RIVGGRPAPA RKWPWQVSLQ VHKQHICGGS LISKWWVITA AHCVYGHLDY AVFMGDADLW SKRPVRIPVQ DIIVHQDFSM MRTVVHDIAL VLLAFPVNYS VNIQPVCIPE KSFLVQPGTL CWVTGWGKVL EQGRSSRILQ EIELNIIRHE KCNQILKDIM GNIFTLVQEG GVCGYNEKGG DACQGDSGGP LVCEFNKTWV QVGIVSWGLG CGRIGYPGVY TEVSYYRDWI IKELSRASCW KLSGFLVLSV CLVLHLAIVV AL //