ID Q3ZAS0_MOUSE Unreviewed; 814 AA. AC Q3ZAS0; DT 27-SEP-2005, integrated into UniProtKB/TrEMBL. DT 27-SEP-2005, sequence version 1. DT 25-MAY-2022, entry version 143. DE RecName: Full=Sodium/hydrogen exchanger {ECO:0000256|RuleBase:RU003722}; GN Name=Slc9a2 {ECO:0000313|EMBL:AAI03686.1, GN ECO:0000313|Ensembl:ENSMUSP00000027231, ECO:0000313|MGI:MGI:105075}; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090 {ECO:0000313|EMBL:AAI03686.1}; RN [1] {ECO:0000313|EMBL:AAI03686.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=PCR rescued clones {ECO:0000313|EMBL:AAI03686.1}; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RA Gerhard D.S., Wagner L., Feingold E.A., Shenmen C.M., Grouse L.H., RA Schuler G., Klein S.L., Old S., Rasooly R., Good P., Guyer M., Peck A.M., RA Derge J.G., Lipman D., Collins F.S., Jang W., Sherry S., Feolo M., RA Misquitta L., Lee E., Rotmistrovsky K., Greenhut S.F., Schaefer C.F., RA Buetow K., Bonner T.I., Haussler D., Kent J., Kiekhaus M., Furey T., RA Brent M., Prange C., Schreiber K., Shapiro N., Bhat N.K., Hopkins R.F., RA Hsie F., Driscoll T., Soares M.B., Casavant T.L., Scheetz T.E., RA Brown-stein M.J., Usdin T.B., Toshiyuki S., Carninci P., Piao Y., RA Dudekula D.B., Ko M.S., Kawakami K., Suzuki Y., Sugano S., Gruber C.E., RA Smith M.R., Simmons B., Moore T., Waterman R., Johnson S.L., Ruan Y., RA Wei C.L., Mathavan S., Gunaratne P.H., Wu J., Garcia A.M., Hulyk S.W., RA Fuh E., Yuan Y., Sneed A., Kowis C., Hodgson A., Muzny D.M., McPherson J., RA Gibbs R.A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., RA Sanchez A., Whiting M., Madari A., Young A.C., Wetherby K.D., Granite S.J., RA Kwong P.N., Brinkley C.P., Pearson R.L., Bouffard G.G., Blakesly R.W., RA Green E.D., Dickson M.C., Rodriguez A.C., Grimwood J., Schmutz J., RA Myers R.M., Butterfield Y.S., Griffith M., Griffith O.L., Krzywinski M.I., RA Liao N., Morin R., Morrin R., Palmquist D., Petrescu A.S., Skalska U., RA Smailus D.E., Stott J.M., Schnerch A., Schein J.E., Jones S.J., Holt R.A., RA Baross A., Marra M.A., Clifton S., Makowski K.A., Bosak S., Malek J.; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [2] {ECO:0000313|Ensembl:ENSMUSP00000027231, ECO:0000313|Proteomes:UP000000589} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000027231, RC ECO:0000313|Proteomes:UP000000589}; RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112; RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., RA Eichler E.E., Ponting C.P.; RT "Lineage-specific biology revealed by a finished genome assembly of the RT mouse."; RL PLoS Biol. 7:E1000112-E1000112(2009). RN [3] {ECO:0007829|PubMed:21183079} RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001; RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.; RT "A tissue-specific atlas of mouse protein phosphorylation and expression."; RL Cell 143:1174-1189(2010). RN [4] {ECO:0000313|Ensembl:ENSMUSP00000027231} RP IDENTIFICATION. RC STRAIN=C57BL/6J {ECO:0000313|Ensembl:ENSMUSP00000027231}; RG Ensembl; RL Submitted (FEB-2012) to UniProtKB. CC -!- SUBUNIT: Interacts with CHP1 and CHP2. {ECO:0000256|ARBA:ARBA00011563}. CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi- CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. CC -!- SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1) CC transporter (TC 2.A.36) family. {ECO:0000256|ARBA:ARBA00007367, CC ECO:0000256|RuleBase:RU003722}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BC103685; AAI03686.1; -; mRNA. DR EMBL; BC104737; AAI04738.1; -; mRNA. DR RefSeq; NP_001028461.2; NM_001033289.2. DR STRING; 10090.ENSMUSP00000027231; -. DR ProteomicsDB; 336574; -. DR Antibodypedia; 32970; 92 antibodies from 22 providers. DR DNASU; 226999; -. DR Ensembl; ENSMUST00000027231.13; ENSMUSP00000027231.8; ENSMUSG00000026062.13. DR GeneID; 226999; -. DR KEGG; mmu:226999; -. DR UCSC; uc007aun.1; mouse. DR CTD; 6549; -. DR MGI; MGI:105075; Slc9a2. DR VEuPathDB; HostDB:ENSMUSG00000026062; -. DR eggNOG; KOG1966; Eukaryota. DR GeneTree; ENSGT00940000156807; -. DR HOGENOM; CLU_005912_4_3_1; -. DR OMA; DDHETMS; -. DR OrthoDB; 316731at2759; -. DR TreeFam; TF317212; -. DR Reactome; R-MMU-425986; Sodium/Proton exchangers. DR BioGRID-ORCS; 226999; 2 hits in 72 CRISPR screens. DR Proteomes; UP000000589; Chromosome 1. DR Bgee; ENSMUSG00000026062; Expressed in colon and 158 other tissues. DR GO; GO:0005903; C:brush border; TAS:MGI. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central. DR GO; GO:0015386; F:potassium:proton antiporter activity; IBA:GO_Central. DR GO; GO:0015385; F:sodium:proton antiporter activity; IDA:MGI. DR GO; GO:0071805; P:potassium ion transmembrane transport; IBA:GO_Central. DR GO; GO:0008104; P:protein localization; IMP:MGI. DR GO; GO:0051453; P:regulation of intracellular pH; IBA:GO_Central. DR GO; GO:0006885; P:regulation of pH; IDA:MGI. DR GO; GO:0098719; P:sodium ion import across plasma membrane; IBA:GO_Central. DR GO; GO:0006814; P:sodium ion transport; IDA:MGI. DR InterPro; IPR006153; Cation/H_exchanger. DR InterPro; IPR018422; Cation/H_exchanger_CPA1. DR InterPro; IPR001953; Na/H_exchanger_2. DR InterPro; IPR004709; NaH_exchanger. DR InterPro; IPR032103; NHE_CaM-bd. DR PANTHER; PTHR10110; PTHR10110; 1. DR Pfam; PF00999; Na_H_Exchanger; 1. DR Pfam; PF16644; NEXCaM_BD; 1. DR PRINTS; PR01084; NAHEXCHNGR. DR PRINTS; PR01086; NAHEXCHNGR2. DR TIGRFAMs; TIGR00840; b_cpa1; 1. PE 1: Evidence at protein level; KW Antiport {ECO:0000256|RuleBase:RU003722}; KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180}; KW Ion transport {ECO:0000256|RuleBase:RU003722}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius}; KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}; KW Reference proteome {ECO:0000313|Proteomes:UP000000589}; KW Signal {ECO:0000256|SAM:SignalP}; Sodium {ECO:0000256|ARBA:ARBA00023053}; KW Sodium transport {ECO:0000256|ARBA:ARBA00023201, KW ECO:0000256|RuleBase:RU003722}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, KW ECO:0000256|RuleBase:RU003722}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|RuleBase:RU003722}. FT SIGNAL 1..34 FT /evidence="ECO:0000256|SAM:SignalP" FT CHAIN 35..814 FT /note="Sodium/hydrogen exchanger" FT /evidence="ECO:0000256|SAM:SignalP" FT /id="PRO_5015097548" FT TRANSMEM 108..129 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 141..158 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 170..193 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 205..230 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 236..258 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 270..300 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 320..341 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 390..420 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 432..454 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT TRANSMEM 460..484 FT /note="Helical" FT /evidence="ECO:0000256|SAM:Phobius" FT DOMAIN 85..485 FT /note="Na_H_Exchanger" FT /evidence="ECO:0000259|Pfam:PF00999" FT DOMAIN 576..684 FT /note="NEXCaM_BD" FT /evidence="ECO:0000259|Pfam:PF16644" FT REGION 647..710 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 740..814 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 677..691 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 692..710 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 752..766 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 772..787 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 799..814 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 814 AA; 91646 MW; 8543A204ED2528C2 CRC64; MGPRGTAHSV RAPLSWLLLL LLSLQVAVPA GALAETLLDA PRARGASSNP PSPASVVAPG TTPFEESRLP VFTLDYPHVQ IPFEITLWIL LASLAKIGFH LYHKLPTIVP ESCLLIMVGL LLGGIIFGVD EKSPPAMKTD VFFLYLLPPI VLDAGYFMPT RPFFENFGTI FWYAVVGTLW NSIGIGVSLF GICQIEAFGL SDITLLQNLL FGSLISAVDP VAVLAVFENI HVNEQLYILV FGESLLNDAV TVVLYNLFKS FCQMKTIQTV DVFAGIANFF VVGIGGVLIG ILLGFIAAFT TRFTHNIRVI EPLFVFLYSY LSYITAEMFH LSGIMAITAC AMTMNKYVEE NVSQKSYTTI KYFMKMLSSV SETLIFIFMG VSTVGKNHEW NWAFVCFTLA FCLIWRALGV FVLTQVINWF RTIPLTFKDQ FIIAYGGLRG AICFALVFLL PATVFPRKKL FITAAIVVIF FTVFILGITI RPLVEFLDVK RSNKKQQAVS EEIHCRFFDH VKTGIEDVCG HWGHNFWRDK FKKFDDKYLR KLLIRENQPK SSIVSLYKKL EIKHAIEMAE TGMISTVPSF ASLNDCREEK IRKLTPGEMD EIREILSRNL YQIRQRTLSY NRHNLTADTS ERQAKEILIR RRHSLRESLR KDNSSNRERR ASTSTSRYLS LPKNTKLPEK LQKKKKVSNA DGNSSDSDPD VGTTVLNLQP RTRRFLPDQF SKKASQAYKM EWKNEVDVGS VRVPPSVSPA PRSKDGGTQT PGLLRQPLLS KDQRFGRGRE DSLTEDVPPK PPPRLVRRAS EPGNRKGRLG NEKP //