ID HACE1_MOUSE Reviewed; 909 AA. AC Q3U0D9; Q5DTY7; Q8BXY2; Q8R160; DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot. DT 11-OCT-2005, sequence version 1. DT 05-OCT-2010, entry version 44. DE RecName: Full=E3 ubiquitin-protein ligase HACE1; DE EC=6.3.2.-; DE AltName: Full=HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; GN Name=Hace1; Synonyms=Kiaa1320; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; OC Muroidea; Muridae; Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RC STRAIN=NOD; TISSUE=Cerebellum, and Spleen; RX PubMed=16141072; DOI=10.1126/science.1112014; RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., RA Davis M.J., Wilming L.G., Aidinis V., Allen J.E., RA Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., RA Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., RA Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., RA Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., RA di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., RA Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., RA Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., RA Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., RA Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., RA Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., RA Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., RA Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., RA Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., RA Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., RA Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., RA Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., RA Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., RA Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., RA Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., RA Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., RA Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., RA Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., RA Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., RA Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., RA Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., RA Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., RA Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., RA Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., RA Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., RA Hayashizaki Y.; RT "The transcriptional landscape of the mammalian genome."; RL Science 309:1559-1563(2005). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE RP SEQUENCE [LARGE SCALE MRNA] OF 567-838 (ISOFORM 3). RC TISSUE=Mammary tumor; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA RT project: the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 689-838 (ISOFORM 2). RC TISSUE=Embryonic intestine; RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., RA Koga H.; RT "Prediction of the coding sequences of mouse homologues of KIAA gene. RT The complete nucleotide sequences of mouse KIAA-homologous cDNAs RT identified by screening of terminal sequences of cDNA clones randomly RT sampled from size-fractionated libraries."; RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: E3 ubiquitin-protein ligase that may function in CC cellular proteins degradation (By similarity). CC -!- PATHWAY: Protein modification; protein ubiquitination. CC -!- SUBUNIT: Interacts with the 26S proteasomal complex through the CC 20S core proteasomal subunit (By similarity). CC -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity). Endoplasmic CC reticulum (By similarity). Note=A significant portion localizes to CC the endoplasmic reticulum (By similarity). CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=3; CC Name=1; CC IsoId=Q3U0D9-1; Sequence=Displayed; CC Name=2; CC IsoId=Q3U0D9-2; Sequence=VSP_023833, VSP_023834; CC Note=No experimental confirmation available; CC Name=3; CC IsoId=Q3U0D9-3; Sequence=VSP_023832; CC Note=No experimental confirmation available; CC -!- SIMILARITY: Contains 6 ANK repeats. CC -!- SIMILARITY: Contains 1 HECT (E6AP-type E3 ubiquitin-protein CC ligase) domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK042879; BAC31390.1; -; mRNA. DR EMBL; AK156958; BAE33915.1; -; mRNA. DR EMBL; BC025227; AAH25227.1; -; mRNA. DR EMBL; BC120695; AAI20696.1; -; mRNA. DR EMBL; BC120697; AAI20698.1; -; mRNA. DR EMBL; AK220383; BAD90440.1; -; mRNA. DR IPI; IPI00226955; -. DR IPI; IPI00830657; -. DR IPI; IPI00831132; -. DR RefSeq; NP_766061.2; -. DR UniGene; Mm.458633; -. DR ProteinModelPortal; Q3U0D9; -. DR SMR; Q3U0D9; 31-238, 67-251, 523-899. DR STRING; Q3U0D9; -. DR PhosphoSite; Q3U0D9; -. DR PRIDE; Q3U0D9; -. DR Ensembl; ENSMUST00000037044; ENSMUSP00000039206; ENSMUSG00000038822. DR Ensembl; ENSMUST00000059925; ENSMUSP00000060603; ENSMUSG00000038822. DR GeneID; 209462; -. DR KEGG; mmu:209462; -. DR NMPDR; fig|10090.3.peg.13640; -. DR UCSC; uc007fad.1; mouse. DR CTD; 209462; -. DR MGI; MGI:2446110; Hace1. DR eggNOG; roNOG14855; -. DR HOGENOM; HBG358212; -. DR HOVERGEN; HBG004134; -. DR InParanoid; Q3U0D9; -. DR OMA; TSIPPFE; -. DR OrthoDB; EOG9STVR5; -. DR PhylomeDB; Q3U0D9; -. DR NextBio; 372681; -. DR ArrayExpress; Q3U0D9; -. DR Bgee; Q3U0D9; -. DR Genevestigator; Q3U0D9; -. DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell. DR GO; GO:0016881; F:acid-amino acid ligase activity; IEA:InterPro. DR GO; GO:0006464; P:protein modification process; IEA:InterPro. DR InterPro; IPR002110; Ankyrin_rpt. DR InterPro; IPR020683; Ankyrin_rpt-contain_dom. DR InterPro; IPR000569; HECT. DR Gene3D; G3DSA:1.25.40.20; ANK; 1. DR Pfam; PF00023; Ank; 3. DR Pfam; PF00632; HECT; 1. DR SMART; SM00248; ANK; 6. DR SMART; SM00119; HECTc; 1. DR SUPFAM; SSF48403; ANK; 1. DR SUPFAM; SSF56204; HECT; 1. DR PROSITE; PS50297; ANK_REP_REGION; 1. DR PROSITE; PS50088; ANK_REPEAT; 5. DR PROSITE; PS50237; HECT; 1. PE 2: Evidence at transcript level; KW Alternative splicing; ANK repeat; Cytoplasm; Endoplasmic reticulum; KW Ligase; Repeat; Ubl conjugation pathway. FT CHAIN 1 909 E3 ubiquitin-protein ligase HACE1. FT /FTId=PRO_0000280623. FT REPEAT 64 93 ANK 1. FT REPEAT 97 126 ANK 2. FT REPEAT 130 159 ANK 3. FT REPEAT 163 192 ANK 4. FT REPEAT 196 226 ANK 5. FT REPEAT 228 257 ANK 6. FT DOMAIN 574 909 HECT. FT ACT_SITE 876 876 Glycyl thioester intermediate (By FT similarity). FT VAR_SEQ 738 781 Missing (in isoform 3). FT /FTId=VSP_023832. FT VAR_SEQ 838 838 S -> R (in isoform 2). FT /FTId=VSP_023833. FT VAR_SEQ 839 909 Missing (in isoform 2). FT /FTId=VSP_023834. FT CONFLICT 92 92 N -> S (in Ref. 1; BAC31390). SQ SEQUENCE 909 AA; 102114 MW; 804F62A06E9C78A0 CRC64; MERAMEQLNR LTRSLRRART VELPEDNETA VYTLMPMVMA DQHRSVSELL SNSKFDVNYA FGRVKRSLLH IAANCGSVEC LVLLLKKGAN PNYQDISGCT PLHLAARNGQ KKCMSKLLEY SADVNICNNE GLTAIHWLAV NGRTELLHDL VQHVTDVDVE DAMGQTALHV ACQNGHKTTV QCLLDSGADI NRPNVSGATP LYFACSHGQR DTAQILLLRG AKYLPDKNGV TPLDLCVQGG YGQTCEVLIQ YHPRLFQTIV QMTQNEDLRE NMLRQVLQHL SQQSESQYLK ILTGLAEVAT TNGHKLLSLS SNYDAQMKSL LRIVRIFCHV FRIGPSSPSN GIDMGYNGNK TPRSQVFKPL ELLWHSLDEW LVLIATELMK NKEDSTDITS ILLKQKGQDQ EAPSLSAFEP PGPGSYESLP PGPGDSKPEV LAGEQEASAD CQDVISVTAN RLSAVIQAFY MCCSCQMPPG MTSPRFIEFV CKHDEVLKCF VNRNPKIIFD HFHFLLECPE LMSRFMHIIK AQPFKDRCEW FYEHLHSGQP DSDMVHRPVS ENDILLVHRD SIFRSSCEIV SKANCAKLKQ GIAVRFHGEE GMGQGVVREW FDILSNEIVN PDYALFTQSA DGTTFQPNSN SYVNPDHLNY FRFAGQILGL ALNHRQLVNI YFTRSFYKHI LGIPVNYQDV ASIDPEYAKN LQWILDNDIS DLGLELTFSV ETDVFGAMEE VPLKPGGGSI LVTQNNKAEY VQLVTELRMT RAIQPQINAF LQGFHMFIPP SLIQLFDEYE LELLLSGMPE IDVNDWIKNT EYTSGYERED PVIQWFWEVV EDITQEERVL LLQFVTGSSR VPHGGFANIM GGSGLQNFTI AAVPYTPNLL PTSSTCINML KLPEYPSKEI LKDRLLVALH CGSYGYTMA //