ID HACE1_MOUSE Reviewed; 909 AA. AC Q3U0D9; F6VQI5; F7ALT5; Q5DTY7; Q8BXY2; Q8R160; Q8R3G4; DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot. DT 11-OCT-2005, sequence version 1. DT 26-FEB-2020, entry version 115. DE RecName: Full=E3 ubiquitin-protein ligase HACE1; DE EC=2.3.2.26; DE AltName: Full=HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; DE AltName: Full=HECT-type E3 ubiquitin transferase HACE1; GN Name=Hace1; Synonyms=Kiaa1320; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). RC STRAIN=NOD; TISSUE=Cerebellum, and Spleen; RX PubMed=16141072; DOI=10.1126/science.1112014; RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.; RT "The transcriptional landscape of the mammalian genome."; RL Science 309:1559-1563(2005). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C57BL/6J; RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112; RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., RA Eichler E.E., Ponting C.P.; RT "Lineage-specific biology revealed by a finished genome assembly of the RT mouse."; RL PLoS Biol. 7:E1000112-E1000112(2009). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE RP [LARGE SCALE MRNA] OF 567-838 (ISOFORM 3). RC TISSUE=Mammary tumor; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 689-838 (ISOFORM 2), AND RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 519-909 (ISOFORM 4). RC TISSUE=Embryonic intestine; RA Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., RA Koga H.; RT "Prediction of the coding sequences of mouse homologues of KIAA gene. The RT complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by RT screening of terminal sequences of cDNA clones randomly sampled from size- RT fractionated libraries."; RL Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases. RN [5] RP DISRUPTION PHENOTYPE. RX PubMed=17694067; DOI=10.1038/nm1621; RA Zhang L., Anglesio M.S., O'Sullivan M., Zhang F., Yang G., Sarao R., RA Mai P.N., Cronin S., Hara H., Melnyk N., Li L., Wada T., Liu P.P., RA Farrar J., Arceci R.J., Sorensen P.H., Penninger J.M.; RT "The E3 ligase HACE1 is a critical chromosome 6q21 tumor suppressor RT involved in multiple cancers."; RL Nat. Med. 13:1060-1069(2007). RN [6] RP INTERACTION WITH RARB, AND FUNCTION. RX PubMed=19350571; DOI=10.1002/jcb.22146; RA Zhao J., Zhang Z., Vucetic Z., Soprano K.J., Soprano D.R.; RT "HACE1: A novel repressor of RAR transcriptional activity."; RL J. Cell. Biochem. 107:482-493(2009). RN [7] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC TISSUE=Brain, Lung, Spleen, and Testis; RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001; RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.; RT "A tissue-specific atlas of mouse protein phosphorylation and expression."; RL Cell 143:1174-1189(2010). CC -!- FUNCTION: E3 ubiquitin-protein ligase involved in Golgi membrane fusion CC and regulation of small GTPases. Acts as a regulator of Golgi membrane CC dynamics during the cell cycle: recruited to Golgi membrane by Rab CC proteins and regulates postmitotic Golgi membrane fusion. Acts by CC mediating ubiquitination during mitotic Golgi disassembly, CC ubiquitination serving as a signal for Golgi reassembly later, after CC cell division. Specifically interacts with GTP-bound RAC1, mediating CC ubiquitination and subsequent degradation of active RAC1, thereby CC playing a role in host defense against pathogens (By similarity). May CC also act as a transcription regulator via its interaction with RARB. CC {ECO:0000250|UniProtKB:Q8IYU2, ECO:0000269|PubMed:19350571}. CC -!- CATALYTIC ACTIVITY: CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; CC EC=2.3.2.26; CC -!- PATHWAY: Protein modification; protein ubiquitination. CC -!- SUBUNIT: Interacts with RAB1 (RAB1A, RAB1B or RAB1C), RAB4 (RAB4A or CC RAB4B) and RAB11 (RAB11A or RAB11B); in a GTP-dependent manner. CC Interacts with RAC1; in a GTP-dependent manner. Interacts with the 26S CC proteasomal complex through the 20S core proteasomal subunit (By CC similarity). Interacts with RARB. {ECO:0000250|UniProtKB:Q8IYU2, CC ECO:0000269|PubMed:19350571}. CC -!- SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane CC {ECO:0000250}. Cytoplasm {ECO:0000250}. Endoplasmic reticulum CC {ECO:0000250}. Note=A significant portion localizes to the endoplasmic CC reticulum. Targeted to Golgi membrane via its interaction with Rab CC proteins (By similarity). {ECO:0000250}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=4; CC Name=1; CC IsoId=Q3U0D9-1; Sequence=Displayed; CC Name=2; CC IsoId=Q3U0D9-2; Sequence=VSP_023833, VSP_023834; CC Name=3; CC IsoId=Q3U0D9-3; Sequence=VSP_023832; CC Name=4; CC IsoId=Q3U0D9-4; Sequence=VSP_023832, VSP_042379; CC -!- DISRUPTION PHENOTYPE: Mice develop spontaneous, late-onset cancer. CC Moreover, tumor incidence in mice heterozygous for a p53/Tp53 mutation CC in higher in a Hace1-deficient background. CC {ECO:0000269|PubMed:17694067}. CC -!- SEQUENCE CAUTION: CC Sequence=AAH25474.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AK042879; BAC31390.1; -; mRNA. DR EMBL; AK156958; BAE33915.1; -; mRNA. DR EMBL; AC135669; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; AC153847; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; BC025227; AAH25227.1; -; mRNA. DR EMBL; BC025474; AAH25474.1; ALT_INIT; mRNA. DR EMBL; BC120695; AAI20696.1; -; mRNA. DR EMBL; BC120697; AAI20698.1; -; mRNA. DR EMBL; AK220383; BAD90440.1; -; mRNA. DR CCDS; CCDS23829.1; -. [Q3U0D9-1] DR RefSeq; NP_766061.2; NM_172473.3. [Q3U0D9-1] DR SMR; Q3U0D9; -. DR BioGrid; 229081; 1. DR IntAct; Q3U0D9; 1. DR STRING; 10090.ENSMUSP00000039206; -. DR iPTMnet; Q3U0D9; -. DR PhosphoSitePlus; Q3U0D9; -. DR MaxQB; Q3U0D9; -. DR PaxDb; Q3U0D9; -. DR PeptideAtlas; Q3U0D9; -. DR PRIDE; Q3U0D9; -. DR Ensembl; ENSMUST00000037044; ENSMUSP00000039206; ENSMUSG00000038822. [Q3U0D9-1] DR GeneID; 209462; -. DR KEGG; mmu:209462; -. DR UCSC; uc007fad.2; mouse. [Q3U0D9-1] DR CTD; 57531; -. DR MGI; MGI:2446110; Hace1. DR eggNOG; KOG0939; Eukaryota. DR eggNOG; KOG4177; Eukaryota. DR eggNOG; COG0666; LUCA. DR eggNOG; COG5021; LUCA. DR GeneTree; ENSGT00940000155839; -. DR HOGENOM; CLU_015878_0_0_1; -. DR InParanoid; Q3U0D9; -. DR KO; K12166; -. DR OrthoDB; 799706at2759; -. DR PhylomeDB; Q3U0D9; -. DR TreeFam; TF323417; -. DR Reactome; R-MMU-983168; Antigen processing: Ubiquitination & Proteasome degradation. DR UniPathway; UPA00143; -. DR ChiTaRS; Hace1; mouse. DR PRO; PR:Q3U0D9; -. DR Proteomes; UP000000589; Chromosome 10. DR RNAct; Q3U0D9; protein. DR Bgee; ENSMUSG00000038822; Expressed in liver and 263 other tissues. DR ExpressionAtlas; Q3U0D9; baseline and differential. DR Genevisible; Q3U0D9; MM. DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI. DR GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell. DR GO; GO:0000139; C:Golgi membrane; ISS:UniProtKB. DR GO; GO:0016604; C:nuclear body; ISO:MGI. DR GO; GO:0017137; F:Rab GTPase binding; ISS:UniProtKB. DR GO; GO:0048365; F:Rac GTPase binding; ISO:MGI. DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central. DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:UniProtKB. DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW. DR GO; GO:0007030; P:Golgi organization; ISS:UniProtKB. DR GO; GO:0061025; P:membrane fusion; ISS:UniProtKB. DR GO; GO:0045732; P:positive regulation of protein catabolic process; IBA:GO_Central. DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central. DR GO; GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB. DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central. DR GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB. DR GO; GO:0030334; P:regulation of cell migration; ISS:UniProtKB. DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; ISS:UniProtKB. DR CDD; cd00078; HECTc; 1. DR Gene3D; 1.25.40.20; -; 2. DR InterPro; IPR002110; Ankyrin_rpt. DR InterPro; IPR020683; Ankyrin_rpt-contain_dom. DR InterPro; IPR036770; Ankyrin_rpt-contain_sf. DR InterPro; IPR000569; HECT_dom. DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase. DR Pfam; PF12796; Ank_2; 1. DR Pfam; PF00632; HECT; 1. DR PRINTS; PR01415; ANKYRIN. DR SMART; SM00248; ANK; 6. DR SMART; SM00119; HECTc; 1. DR SUPFAM; SSF48403; SSF48403; 1. DR SUPFAM; SSF56204; SSF56204; 1. DR PROSITE; PS50297; ANK_REP_REGION; 1. DR PROSITE; PS50088; ANK_REPEAT; 5. DR PROSITE; PS50237; HECT; 1. PE 1: Evidence at protein level; KW Alternative splicing; ANK repeat; Cell cycle; Cytoplasm; KW Endoplasmic reticulum; Golgi apparatus; Membrane; Reference proteome; KW Repeat; Transcription; Transcription regulation; Transferase; KW Ubl conjugation pathway. FT CHAIN 1..909 FT /note="E3 ubiquitin-protein ligase HACE1" FT /id="PRO_0000280623" FT REPEAT 64..93 FT /note="ANK 1" FT REPEAT 97..126 FT /note="ANK 2" FT REPEAT 130..159 FT /note="ANK 3" FT REPEAT 163..192 FT /note="ANK 4" FT REPEAT 196..226 FT /note="ANK 5" FT REPEAT 228..257 FT /note="ANK 6" FT DOMAIN 574..909 FT /note="HECT" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00104" FT ACT_SITE 876 FT /note="Glycyl thioester intermediate" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00104" FT VAR_SEQ 738..781 FT /note="Missing (in isoform 3 and isoform 4)" FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.4" FT /id="VSP_023832" FT VAR_SEQ 814..832 FT /note="Missing (in isoform 4)" FT /evidence="ECO:0000303|Ref.4" FT /id="VSP_042379" FT VAR_SEQ 838 FT /note="S -> R (in isoform 2)" FT /evidence="ECO:0000303|Ref.4" FT /id="VSP_023833" FT VAR_SEQ 839..909 FT /note="Missing (in isoform 2)" FT /evidence="ECO:0000303|Ref.4" FT /id="VSP_023834" FT CONFLICT 92 FT /note="N -> S (in Ref. 1; BAC31390)" FT /evidence="ECO:0000305" SQ SEQUENCE 909 AA; 102114 MW; 804F62A06E9C78A0 CRC64; MERAMEQLNR LTRSLRRART VELPEDNETA VYTLMPMVMA DQHRSVSELL SNSKFDVNYA FGRVKRSLLH IAANCGSVEC LVLLLKKGAN PNYQDISGCT PLHLAARNGQ KKCMSKLLEY SADVNICNNE GLTAIHWLAV NGRTELLHDL VQHVTDVDVE DAMGQTALHV ACQNGHKTTV QCLLDSGADI NRPNVSGATP LYFACSHGQR DTAQILLLRG AKYLPDKNGV TPLDLCVQGG YGQTCEVLIQ YHPRLFQTIV QMTQNEDLRE NMLRQVLQHL SQQSESQYLK ILTGLAEVAT TNGHKLLSLS SNYDAQMKSL LRIVRIFCHV FRIGPSSPSN GIDMGYNGNK TPRSQVFKPL ELLWHSLDEW LVLIATELMK NKEDSTDITS ILLKQKGQDQ EAPSLSAFEP PGPGSYESLP PGPGDSKPEV LAGEQEASAD CQDVISVTAN RLSAVIQAFY MCCSCQMPPG MTSPRFIEFV CKHDEVLKCF VNRNPKIIFD HFHFLLECPE LMSRFMHIIK AQPFKDRCEW FYEHLHSGQP DSDMVHRPVS ENDILLVHRD SIFRSSCEIV SKANCAKLKQ GIAVRFHGEE GMGQGVVREW FDILSNEIVN PDYALFTQSA DGTTFQPNSN SYVNPDHLNY FRFAGQILGL ALNHRQLVNI YFTRSFYKHI LGIPVNYQDV ASIDPEYAKN LQWILDNDIS DLGLELTFSV ETDVFGAMEE VPLKPGGGSI LVTQNNKAEY VQLVTELRMT RAIQPQINAF LQGFHMFIPP SLIQLFDEYE LELLLSGMPE IDVNDWIKNT EYTSGYERED PVIQWFWEVV EDITQEERVL LLQFVTGSSR VPHGGFANIM GGSGLQNFTI AAVPYTPNLL PTSSTCINML KLPEYPSKEI LKDRLLVALH CGSYGYTMA //