ID IF4A1_BOVIN Reviewed; 406 AA. AC Q3SZ54; DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot. DT 11-OCT-2005, sequence version 1. DT 16-MAY-2012, entry version 54. DE RecName: Full=Eukaryotic initiation factor 4A-I; DE Short=eIF-4A-I; DE Short=eIF4A-I; DE EC=3.6.4.13; DE AltName: Full=ATP-dependent RNA helicase eIF4A-1; GN Name=EIF4A1; OS Bos taurus (Bovine). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; OC Pecora; Bovidae; Bovinae; Bos. OX NCBI_TaxID=9913; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=Hereford; TISSUE=Fetal liver; RG NIH - Mammalian Gene Collection (MGC) project; RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: ATP-dependent RNA helicase which is a subunit of the CC eIF4F complex involved in cap recognition and is required for mRNA CC binding to ribosome. In the current model of translation CC initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR CC of mRNAs which is necessary to allow efficient binding of the CC small ribosomal subunit, and subsequent scanning for the initiator CC codon (By similarity). CC -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. CC -!- SUBUNIT: eIF4F is a multi-subunit complex, the composition of CC which varies with external and internal environmental conditions. CC It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. CC Interacts with PAIP1, EIF4E and UPF2. Found in a complex with CC XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact CC with NOM1. Interacts with PDCD4; this interferes with the CC interaction between EIF4A and EIF4G. Interacts with RBM4 (By CC similarity). CC -!- SIMILARITY: Belongs to the DEAD box helicase family. eIF4A CC subfamily. CC -!- SIMILARITY: Contains 1 helicase ATP-binding domain. CC -!- SIMILARITY: Contains 1 helicase C-terminal domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BC103130; AAI03131.1; -; mRNA. DR IPI; IPI00698793; -. DR RefSeq; NP_001029400.1; NM_001034228.1. DR UniGene; Bt.14169; -. DR ProteinModelPortal; Q3SZ54; -. DR SMR; Q3SZ54; 22-400. DR STRING; Q3SZ54; -. DR PRIDE; Q3SZ54; -. DR Ensembl; ENSBTAT00000000144; ENSBTAP00000000144; ENSBTAG00000000132. DR GeneID; 504958; -. DR KEGG; bta:504958; -. DR CTD; 1973; -. DR eggNOG; COG0513; -. DR GeneTree; ENSGT00530000062880; -. DR HOGENOM; HOG000268797; -. DR HOVERGEN; HBG107989; -. DR InParanoid; Q3SZ54; -. DR KO; K03257; -. DR OMA; NCHACIG; -. DR OrthoDB; EOG4640C1; -. DR NextBio; 20866906; -. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro. DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW. DR InterPro; IPR011545; DNA/RNA_helicase_DEAD/DEAH_N. DR InterPro; IPR014001; Helicase_ATP-bd. DR InterPro; IPR001650; Helicase_C. DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS. DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif. DR Pfam; PF00270; DEAD; 1. DR Pfam; PF00271; Helicase_C; 1. DR SMART; SM00487; DEXDc; 1. DR SMART; SM00490; HELICc; 1. DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1. DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1. DR PROSITE; PS51194; HELICASE_CTER; 1. DR PROSITE; PS51195; Q_MOTIF; 1. PE 2: Evidence at transcript level; KW Acetylation; ATP-binding; Complete proteome; Helicase; Hydrolase; KW Initiation factor; Nucleotide-binding; Phosphoprotein; KW Protein biosynthesis; Reference proteome; RNA-binding. FT CHAIN 1 406 Eukaryotic initiation factor 4A-I. FT /FTId=PRO_0000244559. FT DOMAIN 63 234 Helicase ATP-binding. FT DOMAIN 245 406 Helicase C-terminal. FT NP_BIND 76 83 ATP (By similarity). FT MOTIF 32 60 Q motif. FT MOTIF 182 185 DEAD box. FT MOD_RES 118 118 N6-acetyllysine (By similarity). FT MOD_RES 158 158 Phosphothreonine (By similarity). FT MOD_RES 174 174 N6-acetyllysine (By similarity). FT MOD_RES 309 309 N6-acetyllysine (By similarity). SQ SEQUENCE 406 AA; 46154 MW; 6EF89939F3045420 CRC64; MSASQDSRSR DNGPDGMEPE GVIESNWNEI VDSFDDMNLS ESLLRGIYAY GFEKPSAIQQ RAILPCIKGY DVIAQAQSGT GKTATFAISI LQQIELDLKA TQALVLAPTR ELAQQIQKVV MALGDYMGAS CHACIGGTNV RAEVQKLQME APHIIVGTPG RVFDMLNRRY LSPKYIKMFV LDEADEMLSR GFKDQIYDIF QKLNSNTQVV LLSATMPSDV LEVTKKFMRD PIRILVKKEE LTLEGIRQFY INVEREEWKL DTLCDLYETL TITQAVIFIN TRRKVDWLTE KMHARDFTVS AMHGDMDQKE RDVIMREFRS GSSRVLITTD LLARGIDVQQ VSLVINYDLP TNRENYIHRI GRGGRFGRKG VAINMVTEED KRTLRDIETF YNTSIEEMPL NVADLI //