ID   SYL_BURP1               Reviewed;         864 AA.
AC   Q3JNN1;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   31-MAY-2011, entry version 48.
DE   RecName: Full=Leucyl-tRNA synthetase;
DE            EC=6.1.1.4;
DE   AltName: Full=Leucine--tRNA ligase;
DE            Short=LeuRS;
GN   Name=leuS; OrderedLocusNames=BURPS1710b_3451;
OS   Burkholderia pseudomallei (strain 1710b).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; pseudomallei group.
OX   NCBI_TaxID=320372;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1710b;
RA   Woods D.E., Nierman W.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: ATP + L-leucine + tRNA(Leu) = AMP +
CC       diphosphate + L-leucyl-tRNA(Leu).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC       family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABA47866.1; Type=Erroneous initiation;
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DR   EMBL; CP000124; ABA47866.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; YP_334822.1; NC_007434.1.
DR   HSSP; P07813; 2AJH.
DR   STRING; Q3JNN1; -.
DR   GeneID; 3689363; -.
DR   GenomeReviews; CP000124_GR; BURPS1710b_3451.
DR   KEGG; bpm:BURPS1710b_3451; -.
DR   TIGR; BURPS1710b_3451; -.
DR   eggNOG; COG0495; -.
DR   HOGENOM; HBG693845; -.
DR   ProtClustDB; PRK00390; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:EC.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:InterPro.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1; -.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; Aminoacyl-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-synth_Ia_bac/mito.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_1a_anticodon-bd.
DR   InterPro; IPR013155; V/L/I-tRNA-synth_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   Gene3D; G3DSA:3.90.740.10; G3DSA:3.90.740.10; 1.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 3.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 3.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; tRNAsyn_1a_bind; 1.
DR   SUPFAM; SSF50677; ValRS_IleRS_edit; 1.
DR   TIGRFAMs; TIGR00396; LeuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW   Ligase; Nucleotide-binding; Protein biosynthesis.
FT   CHAIN         1    864       Leucyl-tRNA synthetase.
FT                                /FTId=PRO_0000334736.
FT   MOTIF        42     52       "HIGH" region.
FT   MOTIF       624    628       "KMSKS" region.
FT   BINDING     627    627       ATP (By similarity).
SQ   SEQUENCE   864 AA;  96239 MW;  C389C537334F59B5 CRC64;
     MHERYVPADV EAAAQSDWRA ADAYRSKEDA NRKKFYCVSM LPYPSGKLHM GHVRNYTIND
     VMYRYLRMNG YNTLMPMGWD AFGMPAENAA MANGVPPAQW TYENIAYMKK QMQSMGLAID
     WSREVTTCKP DYYKWNQWLF LKMLEKGVAY KKTGTVNWDP VDQTVLANEQ VIDGRGWRSG
     ALVEKREIPM YYMRITQYAD ELLNDLDGLG WPERVKVMQH NWIGKSFGVN FGFPYELDGE
     KKLLRVFTTR ADTIMGVTFC AIAAEHPLAA RLARDKPALQ AFIDECKRGG VAEADIATME
     KKGVATGFSV SHPLTGEPVE VWIGNYVLMS YGEGAVMGVP AHDERDFAFA KKYGLPIRQV
     IAVEGETYST DAWQEWYGDK TRAVCVNSGK YDGLAHDAAV DAIAAELKAG GLGDKQITYR
     LRDWGISRQR YWGTPIPIIH CPSCGDVPVP EQDLPVVLPE DLVPDGTGNP LAKSDAFLNC
     TCPKCGAAAK RETDTMDTFV DSAWYFSRYA APDAQTMVDA RTDYWMPMDQ YIGGIEHAIL
     HLLYSRFWAK VMRDLGLVAF GEPAKNLLTQ GMVLNETFYR EDAAGKKTWY NPADVTVSFD
     DKGRPVGAVL KSDGQPVELG GIEKMSKSKN NGVDPQMLID HYGADTARLF TMFAAPPEQQ
     LEWSGAGVDG ASRFLRRVWA FGFANREALA VRAPFDAAQL AEADKTLRRE IHGVLKQADF
     DYQRLQYNTV VSAAMKMLNA IEGAKGSTPA VLRETYGVLL RVLYPVVPHV TFELWKALGY
     ADEFGPLLDA PWPKVDEAAL EQAEIELVLQ VNGKVRGALK VAKDASREAI EAAAVADEMF
     AKFAEGRPAK KIIVVPGRLV NVVV
//