ID CAL_BRAOT Reviewed; 251 AA. AC Q39375; DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 29-OCT-2014, entry version 72. DE RecName: Full=Transcription factor CAULIFLOWER; DE Short=BoCAL; DE Short=BoiCAL; DE AltName: Full=Agamous-like MADS-box protein CAL; GN Name=CAL; Synonyms=CAL1; OS Brassica oleracea var. italica (Broccoli). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; OC Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; OC Brassica. OX NCBI_TaxID=36774; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE. RC TISSUE=Flower meristem; RX PubMed=7824951; DOI=10.1126/science.7824951; RA Kempin S.A., Savidge B., Yanofsky M.F.; RT "Molecular basis of the cauliflower phenotype in Arabidopsis."; RL Science 267:522-525(1995). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY. RC TISSUE=Flower; RX PubMed=9084216; DOI=10.1007/BF01007702; RA Carr S.M., Irish V.F.; RT "Floral homeotic gene expression defines developmental arrest stages RT in Brassica oleracea L. vars. botrytis and italica."; RL Planta 201:179-188(1997). RN [3] RP REVIEW, AND GENE FAMILY. RX PubMed=10835404; RA Purugganan M.D., Boyles A.L., Suddith J.I.; RT "Variation and selection at the CAULIFLOWER floral homeotic gene RT accompanying the evolution of domesticated Brassica oleracea."; RL Genetics 155:855-862(2000). RN [4] RP DEVELOPMENTAL STAGE. RX PubMed=18332227; DOI=10.1093/jxb/erm327; RA Duclos D.V., Bjoerkman T.; RT "Meristem identity gene expression during curd proliferation and RT flower initiation in Brassica oleracea."; RL J. Exp. Bot. 59:421-433(2008). CC -!- FUNCTION: Probable transcription factor that promotes early floral CC meristem identity in synergy with APETALA1, FRUITFULL and LEAFY. CC Is required subsequently for the transition of an inflorescence CC meristem into a floral meristem. Seems to be partially redundant CC to the function of APETALA1 (By similarity). {ECO:0000250}. CC -!- SUBUNIT: Homodimer capable of binding to CArG-box sequences. CC {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE- CC ProRule:PRU00251}. CC -!- TISSUE SPECIFICITY: Expressed in some of the meristems of arrest- CC stage broccoli heads. {ECO:0000269|PubMed:9084216}. CC -!- DEVELOPMENTAL STAGE: Accumulates in early floral primordia and CC fades out in cells that will give rise to stamens and carpels. CC {ECO:0000269|PubMed:18332227, ECO:0000269|PubMed:7824951}. CC -!- MISCELLANEOUS: CAULIFLOWER may contribute to the shape of the CC floral meristem. This trait has likely been selected by early CC farmers during the domestication of modified inflorescence CC structures. CC -!- SIMILARITY: Contains 1 K-box domain. {ECO:0000255|PROSITE- CC ProRule:PRU00629}. CC -!- SIMILARITY: Contains 1 MADS-box domain. {ECO:0000255|PROSITE- CC ProRule:PRU00251}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; L36926; AAA64790.1; -; mRNA. DR EMBL; U67454; AAB08878.1; -; mRNA. DR PIR; T14456; T14456. DR ProteinModelPortal; Q39375; -. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro. DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW. DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW. DR InterPro; IPR002487; TF_Kbox. DR InterPro; IPR002100; TF_MADSbox. DR Pfam; PF01486; K-box; 1. DR Pfam; PF00319; SRF-TF; 1. DR PRINTS; PR00404; MADSDOMAIN. DR SMART; SM00432; MADS; 1. DR SUPFAM; SSF55455; SSF55455; 1. DR PROSITE; PS51297; K_BOX; 1. DR PROSITE; PS00350; MADS_BOX_1; 1. DR PROSITE; PS50066; MADS_BOX_2; 1. PE 2: Evidence at transcript level; KW Activator; Coiled coil; Developmental protein; Differentiation; KW DNA-binding; Flowering; Nucleus; Transcription; KW Transcription regulation. FT CHAIN 1 251 Transcription factor CAULIFLOWER. FT /FTId=PRO_0000417144. FT DOMAIN 1 61 MADS-box. {ECO:0000255|PROSITE- FT ProRule:PRU00251}. FT DOMAIN 90 180 K-box. {ECO:0000255|PROSITE- FT ProRule:PRU00629}. SQ SEQUENCE 251 AA; 29524 MW; 1E940476BC1E4032 CRC64; MGRGRVEMKR IENKINRQVT FSKRRAGLLK KAHEISILCD AEVSLIVFSH KGKLFEYSSE SCMEKVLEHY ERYSYAEKQL KVPDSHVNAQ TNWSVEYSRL KAKIELLERN QRHYLGEDLE SISIKELQNL EQQLDTSLKH IRSRKNQLMH ESLNHLQRKE KEILEENSML AKQIRERESI LRTHQNQSEQ QNRSHHVAPQ PQPQLNPYMA SSPFLNMGGM YQGEYPTAVR RNRLDLTLEP IYNCNLGYFA A //