ID CAL_BRAOT Reviewed; 251 AA. AC Q39375; DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 02-OCT-2024, entry version 101. DE RecName: Full=Transcription factor CAULIFLOWER; DE Short=BoCAL; DE Short=BoiCAL; DE AltName: Full=Agamous-like MADS-box protein CAL; GN Name=CAL; Synonyms=CAL1; OS Brassica oleracea var. italica (Broccoli). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica. OX NCBI_TaxID=36774; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE. RC TISSUE=Flower meristem; RX PubMed=7824951; DOI=10.1126/science.7824951; RA Kempin S.A., Savidge B., Yanofsky M.F.; RT "Molecular basis of the cauliflower phenotype in Arabidopsis."; RL Science 267:522-525(1995). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY. RC TISSUE=Flower; RX PubMed=9084216; DOI=10.1007/bf01007702; RA Carr S.M., Irish V.F.; RT "Floral homeotic gene expression defines developmental arrest stages in RT Brassica oleracea L. vars. botrytis and italica."; RL Planta 201:179-188(1997). RN [3] RP REVIEW, AND GENE FAMILY. RX PubMed=10835404; DOI=10.1093/genetics/155.2.855; RA Purugganan M.D., Boyles A.L., Suddith J.I.; RT "Variation and selection at the CAULIFLOWER floral homeotic gene RT accompanying the evolution of domesticated Brassica oleracea."; RL Genetics 155:855-862(2000). RN [4] RP DEVELOPMENTAL STAGE. RX PubMed=18332227; DOI=10.1093/jxb/erm327; RA Duclos D.V., Bjoerkman T.; RT "Meristem identity gene expression during curd proliferation and flower RT initiation in Brassica oleracea."; RL J. Exp. Bot. 59:421-433(2008). CC -!- FUNCTION: Probable transcription factor that promotes early floral CC meristem identity in synergy with APETALA1, FRUITFULL and LEAFY. Is CC required subsequently for the transition of an inflorescence meristem CC into a floral meristem. Seems to be partially redundant to the function CC of APETALA1 (By similarity). {ECO:0000250}. CC -!- SUBUNIT: Homodimer capable of binding to CArG-box sequences. CC {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}. CC -!- TISSUE SPECIFICITY: Expressed in some of the meristems of arrest-stage CC broccoli heads. {ECO:0000269|PubMed:9084216}. CC -!- DEVELOPMENTAL STAGE: Accumulates in early floral primordia and fades CC out in cells that will give rise to stamens and carpels. CC {ECO:0000269|PubMed:18332227, ECO:0000269|PubMed:7824951}. CC -!- MISCELLANEOUS: CAULIFLOWER may contribute to the shape of the floral CC meristem. This trait has likely been selected by early farmers during CC the domestication of modified inflorescence structures. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; L36926; AAA64790.1; -; mRNA. DR EMBL; U67454; AAB08878.1; -; mRNA. DR PIR; T14456; T14456. DR AlphaFoldDB; Q39375; -. DR SMR; Q39375; -. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro. DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro. DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IEA:InterPro. DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW. DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW. DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro. DR CDD; cd00265; MADS_MEF2_like; 1. DR Gene3D; 3.40.1810.10; Transcription factor, MADS-box; 1. DR InterPro; IPR050142; MADS-box/MEF2_TF. DR InterPro; IPR033896; MADS_MEF2-like. DR InterPro; IPR002487; TF_Kbox. DR InterPro; IPR002100; TF_MADSbox. DR InterPro; IPR036879; TF_MADSbox_sf. DR PANTHER; PTHR48019:SF79; MADS-BOX TRANSCRIPTION FACTOR MADS-MC; 1. DR PANTHER; PTHR48019; SERUM RESPONSE FACTOR HOMOLOG; 1. DR Pfam; PF01486; K-box; 1. DR Pfam; PF00319; SRF-TF; 1. DR PRINTS; PR00404; MADSDOMAIN. DR SMART; SM00432; MADS; 1. DR SUPFAM; SSF55455; SRF-like; 1. DR PROSITE; PS51297; K_BOX; 1. DR PROSITE; PS00350; MADS_BOX_1; 1. DR PROSITE; PS50066; MADS_BOX_2; 1. PE 2: Evidence at transcript level; KW Activator; Coiled coil; Developmental protein; Differentiation; KW DNA-binding; Flowering; Nucleus; Transcription; Transcription regulation. FT CHAIN 1..251 FT /note="Transcription factor CAULIFLOWER" FT /id="PRO_0000417144" FT DOMAIN 1..61 FT /note="MADS-box" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251" FT DOMAIN 90..180 FT /note="K-box" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00629" FT REGION 183..207 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 184..207 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" SQ SEQUENCE 251 AA; 29524 MW; 1E940476BC1E4032 CRC64; MGRGRVEMKR IENKINRQVT FSKRRAGLLK KAHEISILCD AEVSLIVFSH KGKLFEYSSE SCMEKVLEHY ERYSYAEKQL KVPDSHVNAQ TNWSVEYSRL KAKIELLERN QRHYLGEDLE SISIKELQNL EQQLDTSLKH IRSRKNQLMH ESLNHLQRKE KEILEENSML AKQIRERESI LRTHQNQSEQ QNRSHHVAPQ PQPQLNPYMA SSPFLNMGGM YQGEYPTAVR RNRLDLTLEP IYNCNLGYFA A //