ID IRT1_ARATH Reviewed; 347 AA. AC Q38856; Q8LBP0; DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot. DT 31-MAY-2011, sequence version 2. DT 22-JUL-2015, entry version 122. DE RecName: Full=Fe(2+) transport protein 1; DE AltName: Full=Fe(II) transport protein 1; DE AltName: Full=Iron-regulated transporter 1; DE Flags: Precursor; GN Name=IRT1; OrderedLocusNames=At4g19690; ORFNames=T16H5.50; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; Gunneridae; OC Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; OC Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=10617198; DOI=10.1038/47134; RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., RA Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., RA Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., RA Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M., RA Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., RA Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., RA Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., RA Langham S.-A., McCullagh B., Bilham L., Robben J., RA van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., RA Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., RA Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., RA Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., RA Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., RA De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., RA van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., RA Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., RA Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., RA Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., RA Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., RA Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., RA Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., RA Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., RA Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., RA Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., RA Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., RA Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., RA Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., RA Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., RA Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., RA Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., RA Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., RA Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., RA Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., RA Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., RA Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., RA Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., RA Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., RA Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., RA Chen E., Marra M.A., Martienssen R., McCombie W.R.; RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis RT thaliana."; RL Nature 402:769-777(1999). RN [2] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RG The Arabidopsis Information Resource (TAIR); RL Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., RA Feldmann K.A.; RT "Full-length cDNA from Arabidopsis thaliana."; RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-347 (ISOFORM 1), AND CHARACTERIZATION. RC STRAIN=cv. Landsberg erecta; RX PubMed=8643627; DOI=10.1073/pnas.93.11.5624; RA Eide D., Broderius M., Fett J., Guerinot M.L.; RT "A novel iron-regulated metal transporter from plants identified by RT functional expression in yeast."; RL Proc. Natl. Acad. Sci. U.S.A. 93:5624-5628(1996). RN [5] RP CHARACTERIZATION, AND METAL-BINDING. RX PubMed=9784581; DOI=10.1007/s002329900442; RA Eng B.H., Guerinot M.L., Eide D., Saier M.H. Jr.; RT "Sequence analyses and phylogenetic characterization of the ZIP family RT of metal ion transport proteins."; RL J. Membr. Biol. 166:1-7(1998). RN [6] RP BROAD CATION RANGE. RX PubMed=10394943; DOI=10.1023/A:1026438615520; RA Korshunova Y.O., Eide D., Clark W.G., Guerinot M.L., Pakrasi H.B.; RT "The IRT1 protein from Arabidopsis thaliana is a metal transporter RT with a broad substrate range."; RL Plant Mol. Biol. 40:37-44(1999). RN [7] RP MUTAGENESIS OF HIS-104; ASP-108; GLU-111; ASP-144; HIS-205; SER-206; RP HIS-232 AND GLU-236. RX PubMed=11035780; DOI=10.1073/pnas.210214197; RA Rogers E.E., Eide D.J., Guerinot M.L.; RT "Altered selectivity in an Arabidopsis metal transporter."; RL Proc. Natl. Acad. Sci. U.S.A. 97:12356-12360(2000). RN [8] RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION RP PHENOTYPE. RX PubMed=12084823; DOI=10.1105/tpc.001388; RA Vert G., Grotz N., Dedaldechamp F., Gaymard F., Guerinot M.L., RA Briat J.-F., Curie C.; RT "IRT1, an Arabidopsis transporter essential for iron uptake from the RT soil and for plant growth."; RL Plant Cell 14:1223-1233(2002). CC -!- FUNCTION: High-affinity iron transporter that plays a key role in CC the uptake of iron from the rhizosphere across the plasma membrane CC in the root epidermal layer. Acts as the principal regulator of CC iron homeostasis in planta. Also mediates the heavy metals uptake CC under iron-deficiency by its ability to transport cobalt, cadmium, CC manganese and/or zinc ions. {ECO:0000269|PubMed:12084823}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12084823}; CC Multi-pass membrane protein {ECO:0000269|PubMed:12084823}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; CC IsoId=Q38856-1; Sequence=Displayed; CC Name=2; CC IsoId=Q38856-2; Sequence=VSP_008361, VSP_008362; CC Note=May be due to an intron retention.; CC -!- TISSUE SPECIFICITY: Expressed in the external cell layers of the CC root including the lateral branching zone. Also detected in CC flowers before pollination. {ECO:0000269|PubMed:12084823}. CC -!- INDUCTION: In roots by iron starvation. CC -!- DISRUPTION PHENOTYPE: Plants exhibit a lethal chlorotic phenotype. CC {ECO:0000269|PubMed:12084823}. CC -!- MISCELLANEOUS: Inhibited by cadmium and Fe(2+) ions and at 100- CC fold excess inhibited by cobalt, manganese and zinc ions. CC -!- SIMILARITY: Belongs to the ZIP transporter (TC 2.A.5) family. CC {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=AAB01678.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; CC Sequence=CAA19686.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; CC Sequence=CAB78971.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AL024486; CAA19686.1; ALT_INIT; Genomic_DNA. DR EMBL; AL161551; CAB78971.1; ALT_INIT; Genomic_DNA. DR EMBL; CP002687; AEE84216.1; -; Genomic_DNA. DR EMBL; CP002687; AEE84217.1; -; Genomic_DNA. DR EMBL; AY087095; AAM64655.1; -; mRNA. DR EMBL; U27590; AAB01678.1; ALT_INIT; mRNA. DR PIR; T04750; T04750. DR RefSeq; NP_567590.3; NM_118089.3. [Q38856-1] DR RefSeq; NP_849546.1; NM_179215.1. [Q38856-2] DR UniGene; At.2319; -. DR ProteinModelPortal; Q38856; -. DR BioGrid; 13006; 2. DR DIP; DIP-60391N; -. DR STRING; 3702.AT4G19690.2; -. DR TCDB; 2.A.5.1.2; the zinc (zn(2+))-iron (fe(2+)) permease (zip) family. DR PaxDb; Q38856; -. DR PRIDE; Q38856; -. DR EnsemblPlants; AT4G19690.2; AT4G19690.2; AT4G19690. [Q38856-1] DR GeneID; 827713; -. DR KEGG; ath:AT4G19690; -. DR GeneFarm; 2370; -. DR TAIR; AT4G19690; -. DR eggNOG; NOG238784; -. DR HOGENOM; HOG000157076; -. DR InParanoid; Q38856; -. DR KO; K14709; -. DR OMA; SIIAKWA; -. DR BioCyc; ARA:AT4G19690-MONOMER; -. DR BioCyc; ARA:GQT-2751-MONOMER; -. DR BioCyc; MetaCyc:AT4G19690-MONOMER; -. DR PRO; PR:Q38856; -. DR Proteomes; UP000006548; Chromosome 4. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005886; C:plasma membrane; IDA:TAIR. DR GO; GO:0005802; C:trans-Golgi network; IDA:TAIR. DR GO; GO:0015088; F:copper uptake transmembrane transporter activity; IGI:TAIR. DR GO; GO:0005381; F:iron ion transmembrane transporter activity; TAS:TAIR. DR GO; GO:0005385; F:zinc ion transmembrane transporter activity; IEA:InterPro. DR GO; GO:0071369; P:cellular response to ethylene stimulus; IEP:TAIR. DR GO; GO:0071281; P:cellular response to iron ion; IEP:TAIR. DR GO; GO:0071732; P:cellular response to nitric oxide; IEP:TAIR. DR GO; GO:0035434; P:copper ion transmembrane transport; IGI:GOC. DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW. DR GO; GO:0034755; P:iron ion transmembrane transport; TAS:GOC. DR GO; GO:0030001; P:metal ion transport; IMP:TAIR. DR GO; GO:0015675; P:nickel cation transport; IMP:TAIR. DR GO; GO:0009617; P:response to bacterium; IEP:TAIR. DR InterPro; IPR003689; ZIP. DR InterPro; IPR004698; Zn/Fe_permease_fun/pln. DR Pfam; PF02535; Zip; 1. DR TIGRFAMs; TIGR00820; zip; 1. PE 1: Evidence at protein level; KW Alternative splicing; Cell membrane; Complete proteome; Ion transport; KW Iron; Iron transport; Membrane; Reference proteome; Signal; KW Transmembrane; Transmembrane helix; Transport. FT SIGNAL 1 22 {ECO:0000255}. FT CHAIN 23 347 Fe(2+) transport protein 1. FT /FTId=PRO_0000041636. FT TOPO_DOM 23 52 Extracellular. {ECO:0000255}. FT TRANSMEM 53 73 Helical. {ECO:0000255}. FT TOPO_DOM 74 84 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 85 105 Helical. {ECO:0000255}. FT TOPO_DOM 106 125 Extracellular. {ECO:0000255}. FT TRANSMEM 126 146 Helical. {ECO:0000255}. FT TOPO_DOM 147 192 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 193 213 Helical. {ECO:0000255}. FT TOPO_DOM 214 224 Extracellular. {ECO:0000255}. FT TRANSMEM 225 245 Helical. {ECO:0000255}. FT TOPO_DOM 246 254 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 255 275 Helical. {ECO:0000255}. FT TOPO_DOM 276 286 Extracellular. {ECO:0000255}. FT TRANSMEM 287 307 Helical. {ECO:0000255}. FT TOPO_DOM 308 326 Cytoplasmic. {ECO:0000255}. FT TRANSMEM 327 347 Helical. {ECO:0000255}. FT REGION 205 206 Heavy metals binding. FT VAR_SEQ 198 211 LELGIIVHSVVIGL -> RTHIYTYRISLYFK (in FT isoform 2). {ECO:0000303|Ref.3}. FT /FTId=VSP_008361. FT VAR_SEQ 212 347 Missing (in isoform 2). FT {ECO:0000303|Ref.3}. FT /FTId=VSP_008362. FT MUTAGEN 104 104 H->A: Suppresses transport. FT {ECO:0000269|PubMed:11035780}. FT MUTAGEN 108 108 D->A: Abolishes iron and manganese FT transport. {ECO:0000269|PubMed:11035780}. FT MUTAGEN 111 111 E->A: Abolishes zinc transport. Abolishes FT iron and manganese transport; when FT associated with A-108. FT {ECO:0000269|PubMed:11035780}. FT MUTAGEN 144 144 D->A: Abolishes iron and manganese FT transport. Reduces cadmium transport. FT {ECO:0000269|PubMed:11035780}. FT MUTAGEN 205 205 H->A,E: Suppresses transport. FT {ECO:0000269|PubMed:11035780}. FT MUTAGEN 206 206 S->A: Suppresses transport. FT {ECO:0000269|PubMed:11035780}. FT MUTAGEN 232 232 H->A: Suppresses transport. FT {ECO:0000269|PubMed:11035780}. FT MUTAGEN 236 236 E->A: Suppresses transport. FT {ECO:0000269|PubMed:11035780}. SQ SEQUENCE 347 AA; 36727 MW; 1F94091668994EA4 CRC64; MASNSALLMK TIFLVLIFVS FAISPATSTA PEECGSESAN PCVNKAKALP LKVIAIFVIL IASMIGVGAP LFSRNVSFLQ PDGNIFTIIK CFASGIILGT GFMHVLPDSF EMLSSICLEE NPWHKFPFSG FLAMLSGLIT LAIDSMATSL YTSKNAVGIM PHGHGHGHGP ANDVTLPIKE DDSSNAQLLR YRVIAMVLEL GIIVHSVVIG LSLGATSDTC TIKGLIAALC FHQMFEGMGL GGCILQAEYT NMKKFVMAFF FAVTTPFGIA LGIALSTVYQ DNSPKALITV GLLNACSAGL LIYMALVDLL AAEFMGPKLQ GSIKMQFKCL IAALLGCGGM SIIAKWA //