ID NIKE_BRUA2 Reviewed; 266 AA. AC Q2YL69; DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot. DT 07-FEB-2006, sequence version 1. DT 18-SEP-2019, entry version 85. DE RecName: Full=Nickel import ATP-binding protein NikE {ECO:0000255|HAMAP-Rule:MF_01712}; DE EC=7.2.2.11 {ECO:0000255|HAMAP-Rule:MF_01712}; GN Name=nikE {ECO:0000255|HAMAP-Rule:MF_01712}; GN OrderedLocusNames=BAB2_0438; OS Brucella abortus (strain 2308). OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Brucellaceae; Brucella. OX NCBI_TaxID=359391; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=2308; RX PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005; RA Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., RA Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.; RT "Whole-genome analyses of speciation events in pathogenic Brucellae."; RL Infect. Immun. 73:8353-8361(2005). CC -!- FUNCTION: Part of the ABC transporter complex NikABCDE involved in CC nickel import. Responsible for energy coupling to the transport CC system. {ECO:0000255|HAMAP-Rule:MF_01712}. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O + Ni(2+)(out) = ADP + H(+) + Ni(2+)(in) + CC phosphate; Xref=Rhea:RHEA:15557, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, CC ChEBI:CHEBI:49786, ChEBI:CHEBI:456216; EC=7.2.2.11; CC Evidence={ECO:0000255|HAMAP-Rule:MF_01712}; CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NikD CC and NikE), two transmembrane proteins (NikB and NikC) and a CC solute-binding protein (NikA). {ECO:0000255|HAMAP-Rule:MF_01712}. CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP- CC Rule:MF_01712}; Peripheral membrane protein {ECO:0000255|HAMAP- CC Rule:MF_01712}. CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Nickel CC importer (TC 3.A.1.5.3) family. {ECO:0000255|HAMAP-Rule:MF_01712}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AM040265; CAJ12604.1; -; Genomic_DNA. DR RefSeq; WP_002965842.1; NZ_KN046823.1. DR SMR; Q2YL69; -. DR PRIDE; Q2YL69; -. DR EnsemblBacteria; CAJ12604; CAJ12604; BAB2_0438. DR GeneID; 29595225; -. DR KEGG; bmf:BAB2_0438; -. DR PATRIC; fig|359391.11.peg.2632; -. DR KO; K10824; -. DR OMA; NPLFTIQ; -. DR BioCyc; BABO359391:G1GJA-2653-MONOMER; -. DR Proteomes; UP000002719; Chromosome II. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0016887; F:ATPase activity; IEA:InterPro. DR GO; GO:0015413; F:ATPase-coupled nickel transmembrane transporter activity; IEA:UniProtKB-UniRule. DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro. DR InterPro; IPR003593; AAA+_ATPase. DR InterPro; IPR003439; ABC_transporter-like. DR InterPro; IPR017871; ABC_transporter_CS. DR InterPro; IPR015858; ABC_transpr_NikE. DR InterPro; IPR014137; Nickel_NikE. DR InterPro; IPR027417; P-loop_NTPase. DR Pfam; PF00005; ABC_tran; 1. DR SMART; SM00382; AAA; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR TIGRFAMs; TIGR02769; nickel_nikE; 1. DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1. DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1. DR PROSITE; PS51248; NIKE; 1. PE 3: Inferred from homology; KW ATP-binding; Cell inner membrane; Cell membrane; Complete proteome; KW Ion transport; Membrane; Nickel; Nickel transport; Nucleotide-binding; KW Reference proteome; Translocase; Transport. FT CHAIN 1 266 Nickel import ATP-binding protein NikE. FT /FTId=PRO_0000260204. FT DOMAIN 4 252 ABC transporter. {ECO:0000255|HAMAP- FT Rule:MF_01712}. FT NP_BIND 45 52 ATP. {ECO:0000255|HAMAP-Rule:MF_01712}. SQ SEQUENCE 266 AA; 29180 MW; CBC0D12AA2731DFB CRC64; MSLISADNIV KIYQSHSLVG ASARKTMLHD ISISIGQGET VALLGRSGCG KSTLARLLVG LERPTSGEVR FRGVPLTKLD RSGMKAFRRE VQLIFQDSPG AVNARSSVRA IIGEPLRHLT SLDETRREER IQELLRLVEL PPEIADRLPA QVSGGQLQRI CIARALAVNP KLIILDEAVS NLDIHLQASA LALLTKLQQE GGIAYLFVTH DLRLVQKFAA RCLVMDEGQI VEEIKTADLD SMRHPASRLL REAVLPPLPV RAVETN //