ID NIKE_BRUA2 Reviewed; 266 AA. AC Q2YL69; DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot. DT 07-FEB-2006, sequence version 1. DT 14-DEC-2011, entry version 49. DE RecName: Full=Nickel import ATP-binding protein NikE; DE EC=3.6.3.24; GN Name=nikE; OrderedLocusNames=BAB2_0438; OS Brucella abortus (strain 2308). OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Brucellaceae; Brucella. OX NCBI_TaxID=359391; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=2308; RX PubMed=16299333; DOI=10.1128/IAI.73.12.8353-8361.2005; RA Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., RA Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.; RT "Whole-genome analyses of speciation events in pathogenic Brucellae."; RL Infect. Immun. 73:8353-8361(2005). CC -!- FUNCTION: Part of the ABC transporter complex NikABCDE involved in CC nickel import. Responsible for energy coupling to the transport CC system (By similarity). CC -!- CATALYTIC ACTIVITY: ATP + H(2)O + Ni(2+)(Out) = ADP + phosphate + CC Ni(2+)(In). CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (NikD CC and NikE), two transmembrane proteins (NikB and NikC) and a CC solute-binding protein (NikA) (Potential). CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane CC protein (By similarity). CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Nickel CC importer (TC 3.A.1.5.3) family. CC -!- SIMILARITY: Contains 1 ABC transporter domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AM040265; CAJ12604.1; -; Genomic_DNA. DR RefSeq; YP_418633.1; NC_007624.1. DR ProteinModelPortal; Q2YL69; -. DR STRING; Q2YL69; -. DR GeneID; 3827147; -. DR GenomeReviews; AM040265_GR; BAB2_0438. DR KEGG; bmf:BAB2_0438; -. DR eggNOG; COG1124; -. DR HOGENOM; HBG758042; -. DR OMA; THREERI; -. DR ProtClustDB; PRK10419; -. DR BioCyc; BMEL359391:BAB2_0438-MONOMER; -. DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0016151; F:nickel ion binding; IEA:InterPro. DR GO; GO:0015413; F:nickel-transporting ATPase activity; IEA:EC. DR HAMAP; MF_01712; ABC_ATPase_NikE; 1; -. DR InterPro; IPR003439; ABC_transporter-like. DR InterPro; IPR017871; ABC_transporter_CS. DR InterPro; IPR015858; ABC_transpr_NikE. DR InterPro; IPR003593; ATPase_AAA+_core. DR InterPro; IPR014137; Nickel_NikE. DR KO; K10824; -. DR Pfam; PF00005; ABC_tran; 1. DR SMART; SM00382; AAA; 1. DR TIGRFAMs; TIGR02769; Nickel_nikE; 1. DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1. DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1. DR PROSITE; PS51248; NIKE; 1. PE 3: Inferred from homology; KW ATP-binding; Cell inner membrane; Cell membrane; Complete proteome; KW Hydrolase; Membrane; Nickel; Nickel transport; Nucleotide-binding; KW Transport. FT CHAIN 1 266 Nickel import ATP-binding protein NikE. FT /FTId=PRO_0000260204. FT DOMAIN 4 252 ABC transporter. FT NP_BIND 45 52 ATP (Potential). SQ SEQUENCE 266 AA; 29180 MW; CBC0D12AA2731DFB CRC64; MSLISADNIV KIYQSHSLVG ASARKTMLHD ISISIGQGET VALLGRSGCG KSTLARLLVG LERPTSGEVR FRGVPLTKLD RSGMKAFRRE VQLIFQDSPG AVNARSSVRA IIGEPLRHLT SLDETRREER IQELLRLVEL PPEIADRLPA QVSGGQLQRI CIARALAVNP KLIILDEAVS NLDIHLQASA LALLTKLQQE GGIAYLFVTH DLRLVQKFAA RCLVMDEGQI VEEIKTADLD SMRHPASRLL REAVLPPLPV RAVETN //