ID NIKE_BRUA2 Reviewed; 266 AA. AC Q2YL69; DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot. DT 07-FEB-2006, sequence version 1. DT 12-JUN-2007, entry version 15. DE Nickel import ATP-binding protein nikE (EC 3.6.3.24). GN Name=nikE; OrderedLocusNames=BAB2_0438; OS Brucella abortus (strain 2308). OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Brucellaceae; Brucella. OX NCBI_TaxID=359391; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RX PubMed=16299333; DOI=10.1128/IAI.73.12.8353-8361.2005; RA Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A., RA Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.; RT "Whole-genome analyses of speciation events in pathogenic Brucellae."; RL Infect. Immun. 73:8353-8361(2005). CC -!- FUNCTION: Part of the ABC transporter complex nikABCDE involved in CC nickel import. Responsible for energy coupling to the transport CC system (By similarity). CC -!- CATALYTIC ACTIVITY: ATP + H(2)O + Ni(2+)(Out) = ADP + phosphate + CC Ni(2+)(In). CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (nikD CC and nikE), two transmembrane proteins (nikB and nikC) and a CC solute-binding protein (nikA) (Probable). CC -!- SUBCELLULAR LOCATION: Cell inner membrane; peripheral membrane CC protein (By similarity). CC -!- SIMILARITY: Belongs to the ABC transporter family. Nickel importer CC (TC 3.A.1.5.3) subfamily. CC -!- SIMILARITY: Contains 1 ABC transporter domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AM040265; CAJ12604.1; -; Genomic_DNA. DR GenomeReviews; AM040265_GR; BAB2_0438. DR KEGG; bmf:BAB2_0438; -. DR GO; GO:0015413; F:nickel-transporting ATPase activity; IEA:HAMAP. DR GO; GO:0015675; P:nickel ion transport; IEA:HAMAP. DR HAMAP; MF_01712; -; 1. DR InterPro; IPR003593; AAA+_ATPase_core. DR InterPro; IPR003439; ABC_transp_like. DR InterPro; IPR014137; Nickel_nikE. DR ProDom; PD000006; ABC_transporter; 1. DR SMART; SM00382; AAA; 1. DR TIGRFAMs; TIGR02769; nickel_nikE; 1. DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1. DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1. DR PROSITE; PS51248; NIKE; 1. KW ATP-binding; Complete proteome; Hydrolase; Inner membrane; Membrane; KW Nickel; Nickel transport; Nucleotide-binding; Transport. FT CHAIN 1 266 Nickel import ATP-binding protein nikE. FT /FTId=PRO_0000260204. FT DOMAIN 4 252 ABC transporter. FT NP_BIND 45 52 ATP (Potential). SQ SEQUENCE 266 AA; 29180 MW; CBC0D12AA2731DFB CRC64; MSLISADNIV KIYQSHSLVG ASARKTMLHD ISISIGQGET VALLGRSGCG KSTLARLLVG LERPTSGEVR FRGVPLTKLD RSGMKAFRRE VQLIFQDSPG AVNARSSVRA IIGEPLRHLT SLDETRREER IQELLRLVEL PPEIADRLPA QVSGGQLQRI CIARALAVNP KLIILDEAVS NLDIHLQASA LALLTKLQQE GGIAYLFVTH DLRLVQKFAA RCLVMDEGQI VEEIKTADLD SMRHPASRLL REAVLPPLPV RAVETN //