ID Q2VT45_DANRE Unreviewed; 152 AA. AC Q2VT45; B1AAQ7; F6NV39; DT 10-JAN-2006, integrated into UniProtKB/TrEMBL. DT 10-JAN-2006, sequence version 1. DT 29-MAY-2024, entry version 133. DE RecName: Full=Relaxin-3 {ECO:0000256|ARBA:ARBA00040355}; GN Name=rln3a {ECO:0000313|Ensembl:ENSDARP00000082236, GN ECO:0000313|RefSeq:NP_001032892.1, GN ECO:0000313|ZFIN:ZDB-GENE-050310-3}; GN Synonyms=RFLC2 {ECO:0000313|EMBL:ACA13585.1, GN ECO:0000313|RefSeq:NP_001032892.1}, rln3 GN {ECO:0000313|RefSeq:NP_001032892.1, GN ECO:0000313|ZFIN:ZDB-GENE-050310-3}, rlx 3a GN {ECO:0000313|RefSeq:NP_001032892.1}, rlx3a GN {ECO:0000313|RefSeq:NP_001032892.1}; OS Danio rerio (Zebrafish) (Brachydanio rerio). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Danionidae; Danioninae; Danio. OX NCBI_TaxID=7955 {ECO:0000313|EMBL:ACA13585.1}; RN [1] {ECO:0000313|EMBL:AAX39710.1} RP NUCLEOTIDE SEQUENCE. RA Park J.I., Hsu S.Y.T.; RT "Characterization and identification of relaxin family in the zebrafish."; RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=15707501; DOI=10.1186/1471-2148-5-14; RA Wilkinson T.N., Speed T.P., Tregear G.W., Bathgate R.A.; RT "Evolution of the relaxin-like peptide family."; RL BMC Evol. Biol. 5:14-14(2005). RN [3] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=18985751; RA Donizetti A., Grossi M., Pariante P., D'Aniello E., Izzo G., Minucci S., RA Aniello F.; RT "Two neuron clusters in the stem of postembryonic zebrafish brain RT specifically express relaxin-3 gene: first evidence of nucleus incertus in RT fish."; RL Dev. Dyn. 237:3864-3869(2008). RN [4] {ECO:0000313|EMBL:ACA13585.1, ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=18463305; DOI=10.1101/gr.7119108; RA Park J.I., Semyonov J., Chang C.L., Yi W., Warren W., Hsu S.Y.; RT "Origin of INSL3-mediated testicular descent in therian mammals."; RL Genome Res. 18:974-985(2008). RN [5] {ECO:0000313|EMBL:ACA13585.1} RP NUCLEOTIDE SEQUENCE. RA Park J., Semyonov J., Chang C.L., Yi W., Warren W., Hsu S.Y.T.; RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases. RN [6] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=19416157; RA Yegorov S., Good-Avila S.V., Parry L., Wilson B.C.; RT "Relaxin family genes in humans and teleosts."; RL Ann. N. Y. Acad. Sci. 1160:42-44(2009). RN [7] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=18852059; DOI=10.1016/j.cbpa.2008.09.020; RA Wilson B.C., Burnett D., Rappaport R., Parry L.J., Fletcher E.K.; RT "Relaxin-3 and RXFP3 expression, and steroidogenic actions in the ovary of RT teleost fish."; RL Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 153:69-74(2009). RN [8] {ECO:0000313|Ensembl:ENSDARP00000082236} RP IDENTIFICATION. RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000082236}; RG Ensembl; RL Submitted (FEB-2012) to UniProtKB. RN [9] {ECO:0000313|Ensembl:ENSDARP00000082236, ECO:0000313|Proteomes:UP000000437} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000082236}; RX PubMed=23594743; DOI=10.1038/nature12111; RG Genome Reference Consortium Zebrafish; RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J., RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D., RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B., RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J., RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D., RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B., RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P., RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., RA Rogers J., Stemple D.L.; RT "The zebrafish reference genome sequence and its relationship to the human RT genome."; RL Nature 496:498-503(2013). RN [10] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=29257949; RA Hui S.P., Sheng D.Z., Sugimoto K., Gonzalez-Rajal A., Nakagawa S., RA Hesselson D., Kikuchi K.; RT "Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative RT Programs."; RL Dev. Cell 43:659-672(2017). RN [11] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=27993976; RA Chiba A., Watanabe-Takano H., Terai K., Fukui H., Miyazaki T., Uemura M., RA Hashimoto H., Hibi M., Fukuhara S., Mochizuki N.; RT "Osteocrin, a peptide secreted from the heart and other tissues, RT contributes to cranial osteogenesis and chondrogenesis in zebrafish."; RL Development 144:334-344(2017). RN [12] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=28252024; RA Bayes A., Collins M.O., Reig-Viader R., Gou G., Goulding D., Izquierdo A., RA Choudhary J.S., Emes R.D., Grant S.G.; RT "Evolution of complexity in the zebrafish synapse proteome."; RL Nat. Commun. 8:14613-14613(2017). RN [13] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=30703485; RA Alnafea H., Vahkal B., Zelmer C.K., Yegorov S., Bogerd J., Good S.V.; RT "Japanese medaka as a model for studying the relaxin family genes involved RT in neuroendocrine regulation: Insights from the expression of fish-specific RT rln3 and insl5 and rxfp3/4-type receptor paralogues."; RL Mol. Cell. Endocrinol. 487:2-11(2019). RN [14] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=31017557; RA Teixeira C.M.M., Correa C.N., Iwai L.K., Ferro E.S., Castro L.M.; RT "Characterization of Intracellular Peptides from Zebrafish (Danio rerio) RT Brain."; RL Zebrafish 16:240-251(2019). RN [15] {ECO:0000313|RefSeq:NP_001032892.1} RP IDENTIFICATION. RG RefSeq; RL Submitted (FEB-2024) to UniProtKB. CC -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two CC disulfide bonds. {ECO:0000256|ARBA:ARBA00011207}. CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613, CC ECO:0000256|RuleBase:RU000406}. CC -!- SIMILARITY: Belongs to the insulin family. CC {ECO:0000256|ARBA:ARBA00009034, ECO:0000256|RuleBase:RU000406}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BX546473; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; AY834223; AAX39710.1; -; mRNA. DR EMBL; EU437454; ACA13585.1; -; mRNA. DR RefSeq; NP_001032892.1; NM_001037803.1. DR STRING; 7955.ENSDARP00000082236; -. DR PaxDb; 7955-ENSDARP00000082236; -. DR Ensembl; ENSDART00000087803.5; ENSDARP00000082236.4; ENSDARG00000070780.4. DR GeneID; 503864; -. DR KEGG; dre:503864; -. DR AGR; ZFIN:ZDB-GENE-050310-3; -. DR CTD; 503864; -. DR ZFIN; ZDB-GENE-050310-3; rln3a. DR eggNOG; ENOG502S2Z0; Eukaryota. DR HOGENOM; CLU_120043_0_0_1; -. DR OMA; CAKEAQP; -. DR OrthoDB; 5353072at2759; -. DR TreeFam; TF333404; -. DR Reactome; R-DRE-418594; G alpha (i) signalling events. DR Reactome; R-DRE-444821; Relaxin receptors. DR Proteomes; UP000000437; Chromosome 3. DR Bgee; ENSDARG00000070780; Expressed in central gray substance of midbrain and 12 other cell types or tissues. DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell. DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central. DR GO; GO:0005179; F:hormone activity; IEA:InterPro. DR CDD; cd04365; IlGF_relaxin_like; 1. DR InterPro; IPR016179; Insulin-like. DR InterPro; IPR036438; Insulin-like_sf. DR InterPro; IPR022353; Insulin_CS. DR InterPro; IPR022352; Insulin_family. DR PANTHER; PTHR20968; ILGF DOMAIN-CONTAINING PROTEIN; 1. DR PANTHER; PTHR20968:SF0; RELAXIN-3; 1. DR Pfam; PF00049; Insulin; 1. DR PRINTS; PR00276; INSULINFAMLY. DR SMART; SM00078; IlGF; 1. DR SUPFAM; SSF56994; Insulin-like; 1. DR PROSITE; PS00262; INSULIN; 1. PE 1: Evidence at protein level; KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157}; KW Proteomics identification {ECO:0007829|PeptideAtlas:Q2VT45}; KW Reference proteome {ECO:0000313|Proteomes:UP000000437}; KW Secreted {ECO:0000256|ARBA:ARBA00022525, ECO:0000256|RuleBase:RU000406}; KW Signal {ECO:0000256|SAM:SignalP, ECO:0000313|RefSeq:NP_001032892.1}. FT SIGNAL 1..19 FT /evidence="ECO:0000256|SAM:SignalP" FT CHAIN 20..152 FT /note="Relaxin-3" FT /evidence="ECO:0000256|SAM:SignalP, FT ECO:0000313|RefSeq:NP_001032892.1" FT /id="PRO_5035034413" FT DOMAIN 28..152 FT /note="Insulin-like" FT /evidence="ECO:0000259|SMART:SM00078" SQ SEQUENCE 152 AA; 16155 MW; FD17964AE9F5E73E CRC64; MMWKTSALVV CLLLAGVKAL DAGPSYGVKL CGREFIRAVI FTCGGSRWKR SLTNTDEILD LFNSYDSNAA DGSVLFGSAS SSSQHATDSN LPPLGQGEQD GSVFSRPARS LISEEVLEAL RSVDRKGRDV VVGLSNACCK WGCSKGEISS LC //