ID Q2VT45_DANRE Unreviewed; 152 AA. AC Q2VT45; F6NV39; DT 10-JAN-2006, integrated into UniProtKB/TrEMBL. DT 10-JAN-2006, sequence version 1. DT 22-FEB-2023, entry version 126. DE SubName: Full=Relaxin 3a {ECO:0000313|EMBL:AAX39710.1}; DE SubName: Full=Relaxin family locus C type 2 {ECO:0000313|EMBL:ACA13585.1}; DE SubName: Full=relaxin 3a precursor {ECO:0000313|RefSeq:NP_001032892.1}; GN Name=RFLC2 {ECO:0000313|EMBL:ACA13585.1, GN ECO:0000313|RefSeq:NP_001032892.1}; GN Synonyms=rln3 {ECO:0000313|RefSeq:NP_001032892.1}, rlx 3a GN {ECO:0000313|RefSeq:NP_001032892.1}, rlx3a GN {ECO:0000313|RefSeq:NP_001032892.1}; GN OrderedLocusNames=rln3a {ECO:0000313|RefSeq:NP_001032892.1, GN ECO:0000313|ZFIN:ZDB-GENE-050310-3}; OS Danio rerio (Zebrafish) (Brachydanio rerio). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Danionidae; Danioninae; Danio. OX NCBI_TaxID=7955 {ECO:0000313|EMBL:ACA13585.1}; RN [1] {ECO:0000313|EMBL:AAX39710.1} RP NUCLEOTIDE SEQUENCE. RA Park J.I., Hsu S.Y.T.; RT "Characterization and identification of relaxin family in the zebrafish."; RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases. RN [2] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=15707501; DOI=10.1186/1471-2148-5-14; RA Wilkinson T.N., Speed T.P., Tregear G.W., Bathgate R.A.; RT "Evolution of the relaxin-like peptide family."; RL BMC Evol. Biol. 5:14-14(2005). RN [3] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=18985751; RA Donizetti A., Grossi M., Pariante P., D'Aniello E., Izzo G., Minucci S., RA Aniello F.; RT "Two neuron clusters in the stem of postembryonic zebrafish brain RT specifically express relaxin-3 gene: first evidence of nucleus incertus in RT fish."; RL Dev. Dyn. 237:3864-3869(2008). RN [4] {ECO:0000313|EMBL:ACA13585.1, ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=18463305; DOI=10.1101/gr.7119108; RA Park J.I., Semyonov J., Chang C.L., Yi W., Warren W., Hsu S.Y.; RT "Origin of INSL3-mediated testicular descent in therian mammals."; RL Genome Res. 18:974-985(2008). RN [5] {ECO:0000313|EMBL:ACA13585.1} RP NUCLEOTIDE SEQUENCE. RA Park J., Semyonov J., Chang C.L., Yi W., Warren W., Hsu S.Y.T.; RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases. RN [6] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=19416157; RA Yegorov S., Good-Avila S.V., Parry L., Wilson B.C.; RT "Relaxin family genes in humans and teleosts."; RL Ann. N. Y. Acad. Sci. 1160:42-44(2009). RN [7] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=18852059; DOI=10.1016/j.cbpa.2008.09.020; RA Wilson B.C., Burnett D., Rappaport R., Parry L.J., Fletcher E.K.; RT "Relaxin-3 and RXFP3 expression, and steroidogenic actions in the ovary of RT teleost fish."; RL Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 153:69-74(2009). RN [8] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=29257949; RA Hui S.P., Sheng D.Z., Sugimoto K., Gonzalez-Rajal A., Nakagawa S., RA Hesselson D., Kikuchi K.; RT "Zebrafish Regulatory T Cells Mediate Organ-Specific Regenerative RT Programs."; RL Dev. Cell 43:659-672(2017). RN [9] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=27993976; RA Chiba A., Watanabe-Takano H., Terai K., Fukui H., Miyazaki T., Uemura M., RA Hashimoto H., Hibi M., Fukuhara S., Mochizuki N.; RT "Osteocrin, a peptide secreted from the heart and other tissues, RT contributes to cranial osteogenesis and chondrogenesis in zebrafish."; RL Development 144:334-344(2017). RN [10] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=28252024; RA Bayes A., Collins M.O., Reig-Viader R., Gou G., Goulding D., Izquierdo A., RA Choudhary J.S., Emes R.D., Grant S.G.; RT "Evolution of complexity in the zebrafish synapse proteome."; RL Nat. Commun. 8:14613-14613(2017). RN [11] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=30703485; RA Alnafea H., Vahkal B., Zelmer C.K., Yegorov S., Bogerd J., Good S.V.; RT "Japanese medaka as a model for studying the relaxin family genes involved RT in neuroendocrine regulation: Insights from the expression of fish-specific RT rln3 and insl5 and rxfp3/4-type receptor paralogues."; RL Mol. Cell. Endocrinol. 487:2-11(2019). RN [12] {ECO:0000313|RefSeq:NP_001032892.1} RP NUCLEOTIDE SEQUENCE. RX PubMed=31017557; RA Teixeira C.M.M., Correa C.N., Iwai L.K., Ferro E.S., Castro L.M.; RT "Characterization of Intracellular Peptides from Zebrafish (Danio rerio) RT Brain."; RL Zebrafish 16:240-251(2019). RN [13] {ECO:0000313|RefSeq:NP_001032892.1} RP IDENTIFICATION. RG RefSeq; RL Submitted (APR-2022) to UniProtKB. CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613, CC ECO:0000256|RuleBase:RU000406}. CC -!- SIMILARITY: Belongs to the insulin family. CC {ECO:0000256|ARBA:ARBA00009034, ECO:0000256|RuleBase:RU000406}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AY834223; AAX39710.1; -; mRNA. DR EMBL; EU437454; ACA13585.1; -; mRNA. DR RefSeq; NP_001032892.1; NM_001037803.1. DR AlphaFoldDB; Q2VT45; -. DR SMR; Q2VT45; -. DR STRING; 7955.ENSDARP00000082236; -. DR GeneID; 503864; -. DR KEGG; dre:503864; -. DR CTD; 503864; -. DR ZFIN; ZDB-GENE-050310-3; rln3a. DR eggNOG; ENOG502S2Z0; Eukaryota. DR HOGENOM; CLU_120043_0_0_1; -. DR OMA; CEWGCSK; -. DR OrthoDB; 5353072at2759; -. DR TreeFam; TF333404; -. DR Proteomes; UP000000437; Chromosome 3. DR Bgee; ENSDARG00000070780; Expressed in central gray substance of midbrain and 12 other tissues. DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell. DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central. DR GO; GO:0005179; F:hormone activity; IEA:InterPro. DR CDD; cd04365; IlGF_relaxin_like; 1. DR InterPro; IPR016179; Insulin-like. DR InterPro; IPR036438; Insulin-like_sf. DR InterPro; IPR022353; Insulin_CS. DR InterPro; IPR022352; Insulin_family. DR PANTHER; PTHR20968; ILGF DOMAIN-CONTAINING PROTEIN; 1. DR PANTHER; PTHR20968:SF0; RELAXIN-3; 1. DR Pfam; PF00049; Insulin; 1. DR PRINTS; PR00276; INSULINFAMLY. DR SMART; SM00078; IlGF; 1. DR SUPFAM; SSF56994; Insulin-like; 1. DR PROSITE; PS00262; INSULIN; 1. PE 1: Evidence at protein level; KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157}; KW Proteomics identification {ECO:0007829|PeptideAtlas:Q2VT45}; KW Reference proteome {ECO:0000313|Proteomes:UP000000437}; KW Secreted {ECO:0000256|ARBA:ARBA00022525, ECO:0000256|RuleBase:RU000406}; KW Signal {ECO:0000256|SAM:SignalP, ECO:0000313|RefSeq:NP_001032892.1}. FT SIGNAL 1..19 FT /evidence="ECO:0000256|SAM:SignalP" FT CHAIN 20..152 FT /evidence="ECO:0000256|SAM:SignalP, FT ECO:0000313|RefSeq:NP_001032892.1" FT /id="PRO_5035034413" FT DOMAIN 28..152 FT /note="Insulin-like" FT /evidence="ECO:0000259|SMART:SM00078" SQ SEQUENCE 152 AA; 16155 MW; FD17964AE9F5E73E CRC64; MMWKTSALVV CLLLAGVKAL DAGPSYGVKL CGREFIRAVI FTCGGSRWKR SLTNTDEILD LFNSYDSNAA DGSVLFGSAS SSSQHATDSN LPPLGQGEQD GSVFSRPARS LISEEVLEAL RSVDRKGRDV VVGLSNACCK WGCSKGEISS LC //