ID   T132A_HUMAN             Reviewed;        1023 AA.
AC   Q24JP5; Q69YU7; Q86VZ8; Q86W97; Q9H8K3; Q9HCI9; Q9NWY0;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   10-FEB-2021, entry version 116.
DE   RecName: Full=Transmembrane protein 132A;
DE   AltName: Full=HSPA5-binding protein 1;
DE   Flags: Precursor;
GN   Name=TMEM132A; Synonyms=HSPA5BP1, KIAA1583;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC   TISSUE=Brain;
RX   PubMed=10997877; DOI=10.1093/dnares/7.4.271;
RA   Nagase T., Kikuno R., Nakayama M., Hirosawa M., Ohara O.;
RT   "Prediction of the coding sequences of unidentified human genes. XVIII. The
RT   complete sequences of 100 new cDNA clones from brain which code for large
RT   proteins in vitro.";
RL   DNA Res. 7:273-281(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4), NUCLEOTIDE SEQUENCE
RP   [LARGE SCALE MRNA] OF 364-1023 (ISOFORM 1), AND VARIANT VAL-969.
RC   TISSUE=Placenta;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP   [LARGE SCALE MRNA] OF 1-560 (ISOFORM 2).
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 188-560 (ISOFORM 1).
RC   TISSUE=Brain;
RX   PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA   Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA   Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA   Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA   Wiemann S., Schupp I.;
RT   "The full-ORF clone resource of the German cDNA consortium.";
RL   BMC Genomics 8:399-399(2007).
RN   [5]
RP   PHOSPHORYLATION AT SER-529.
RX   PubMed=26091039; DOI=10.1016/j.cell.2015.05.028;
RA   Tagliabracci V.S., Wiley S.E., Guo X., Kinch L.N., Durrant E., Wen J.,
RA   Xiao J., Cui J., Nguyen K.B., Engel J.L., Coon J.J., Grishin N.,
RA   Pinna L.A., Pagliarini D.J., Dixon J.E.;
RT   "A single kinase generates the majority of the secreted phosphoproteome.";
RL   Cell 161:1619-1632(2015).
CC   -!- FUNCTION: May play a role in embryonic and postnatal development of the
CC       brain. Increased resistance to cell death induced by serum starvation
CC       in cultured cells. Regulates cAMP-induced GFAP gene expression via
CC       STAT3 phosphorylation (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with HSPA5/GRP78. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC       pass type I membrane protein {ECO:0000250}. Endoplasmic reticulum
CC       membrane {ECO:0000250}; Single-pass type I membrane protein
CC       {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=4;
CC       Name=1;
CC         IsoId=Q24JP5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q24JP5-2; Sequence=VSP_025304;
CC       Name=3;
CC         IsoId=Q24JP5-3; Sequence=VSP_025302, VSP_025305, VSP_025306;
CC       Name=4;
CC         IsoId=Q24JP5-4; Sequence=VSP_025303, VSP_025304;
CC   -!- SIMILARITY: Belongs to the TMEM132 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB13409.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAB14613.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AB046803; BAB13409.1; ALT_INIT; mRNA.
DR   EMBL; AK000546; BAA91245.1; -; mRNA.
DR   EMBL; AK023577; BAB14613.1; ALT_INIT; mRNA.
DR   EMBL; BC028106; AAH28106.1; -; mRNA.
DR   EMBL; BC032059; AAH32059.1; -; mRNA.
DR   EMBL; BC114569; AAI14570.1; -; mRNA.
DR   EMBL; AL359557; CAH10668.2; -; mRNA.
DR   CCDS; CCDS44618.1; -. [Q24JP5-1]
DR   CCDS; CCDS7997.1; -. [Q24JP5-2]
DR   RefSeq; NP_060340.2; NM_017870.3. [Q24JP5-2]
DR   RefSeq; NP_821174.1; NM_178031.2. [Q24JP5-1]
DR   BioGRID; 120309; 74.
DR   IntAct; Q24JP5; 59.
DR   STRING; 9606.ENSP00000005286; -.
DR   TCDB; 8.A.143.1.1; the tmem132 (tmem132) family.
DR   GlyGen; Q24JP5; 3 sites, 1 O-linked glycan (1 site).
DR   iPTMnet; Q24JP5; -.
DR   PhosphoSitePlus; Q24JP5; -.
DR   BioMuta; TMEM132A; -.
DR   DMDM; 121940967; -.
DR   EPD; Q24JP5; -.
DR   jPOST; Q24JP5; -.
DR   MassIVE; Q24JP5; -.
DR   MaxQB; Q24JP5; -.
DR   PaxDb; Q24JP5; -.
DR   PeptideAtlas; Q24JP5; -.
DR   PRIDE; Q24JP5; -.
DR   ProteomicsDB; 61259; -. [Q24JP5-1]
DR   ProteomicsDB; 61260; -. [Q24JP5-2]
DR   ProteomicsDB; 61261; -. [Q24JP5-3]
DR   ProteomicsDB; 61262; -. [Q24JP5-4]
DR   Antibodypedia; 52906; 70 antibodies.
DR   Ensembl; ENST00000005286; ENSP00000005286; ENSG00000006118. [Q24JP5-2]
DR   Ensembl; ENST00000453848; ENSP00000405823; ENSG00000006118. [Q24JP5-1]
DR   GeneID; 54972; -.
DR   KEGG; hsa:54972; -.
DR   UCSC; uc001nqi.4; human. [Q24JP5-1]
DR   CTD; 54972; -.
DR   DisGeNET; 54972; -.
DR   GeneCards; TMEM132A; -.
DR   HGNC; HGNC:31092; TMEM132A.
DR   HPA; ENSG00000006118; Tissue enhanced (brain).
DR   MIM; 617363; gene.
DR   neXtProt; NX_Q24JP5; -.
DR   OpenTargets; ENSG00000006118; -.
DR   PharmGKB; PA134886953; -.
DR   VEuPathDB; HostDB:ENSG00000006118.14; -.
DR   eggNOG; KOG4789; Eukaryota.
DR   GeneTree; ENSGT00940000161414; -.
DR   HOGENOM; CLU_009871_0_0_1; -.
DR   InParanoid; Q24JP5; -.
DR   OMA; TEHVGCE; -.
DR   OrthoDB; 598074at2759; -.
DR   PhylomeDB; Q24JP5; -.
DR   TreeFam; TF314981; -.
DR   PathwayCommons; Q24JP5; -.
DR   Reactome; R-HSA-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR   Reactome; R-HSA-8957275; Post-translational protein phosphorylation.
DR   BioGRID-ORCS; 54972; 33 hits in 877 CRISPR screens.
DR   ChiTaRS; TMEM132A; human.
DR   GenomeRNAi; 54972; -.
DR   Pharos; Q24JP5; Tdark.
DR   PRO; PR:Q24JP5; -.
DR   Proteomes; UP000005640; Chromosome 11.
DR   RNAct; Q24JP5; protein.
DR   Bgee; ENSG00000006118; Expressed in cortical plate and 193 other tissues.
DR   ExpressionAtlas; Q24JP5; baseline and differential.
DR   Genevisible; Q24JP5; HS.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR   GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
DR   GO; GO:0043687; P:post-translational protein modification; TAS:Reactome.
DR   InterPro; IPR026307; TMEM132.
DR   InterPro; IPR031436; TMEM132_C.
DR   InterPro; IPR031437; TMEM132_M.
DR   InterPro; IPR031435; TMEM132_N.
DR   PANTHER; PTHR13388; PTHR13388; 1.
DR   Pfam; PF16070; TMEM132; 1.
DR   Pfam; PF15706; TMEM132D_C; 1.
DR   Pfam; PF15705; TMEM132D_N; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Endoplasmic reticulum; Glycoprotein; Golgi apparatus;
KW   Membrane; Phosphoprotein; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000255"
FT   CHAIN           36..1023
FT                   /note="Transmembrane protein 132A"
FT                   /id="PRO_0000287096"
FT   TOPO_DOM        36..852
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        853..873
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        874..1023
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          611..916
FT                   /note="Binds to HSPA5/GRP78"
FT                   /evidence="ECO:0000250"
FT   REGION          671..1023
FT                   /note="Confers cellular localization similar to full-length
FT                   form"
FT                   /evidence="ECO:0000250"
FT   COMPBIAS        812..835
FT                   /note="Glu-rich"
FT   MOD_RES         529
FT                   /note="Phosphoserine; by FAM20C"
FT                   /evidence="ECO:0000269|PubMed:26091039"
FT   CARBOHYD        280
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..33
FT                   /note="MCARMAGRTTAAPRGPYGPWLCLLVALALDVVR -> MAPGSAPASSGAVII
FT                   SPSYASS (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:10997877"
FT                   /id="VSP_025302"
FT   VAR_SEQ         46..295
FT                   /note="Missing (in isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_025303"
FT   VAR_SEQ         337
FT                   /note="D -> DS (in isoform 2 and isoform 4)"
FT                   /evidence="ECO:0000303|PubMed:14702039,
FT                   ECO:0000303|PubMed:15489334"
FT                   /id="VSP_025304"
FT   VAR_SEQ         405..423
FT                   /note="AEELVNTAPLTGVPQHVPV -> VRRPPSLPGKAGGQVPGAP (in
FT                   isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:10997877"
FT                   /id="VSP_025305"
FT   VAR_SEQ         424..1023
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000303|PubMed:10997877"
FT                   /id="VSP_025306"
FT   VARIANT         699
FT                   /note="R -> H (in dbSNP:rs524523)"
FT                   /id="VAR_032262"
FT   VARIANT         969
FT                   /note="A -> V (in dbSNP:rs2469887)"
FT                   /evidence="ECO:0000269|PubMed:14702039"
FT                   /id="VAR_032263"
FT   CONFLICT        10
FT                   /note="T -> R (in Ref. 2; BAA91245)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        824
FT                   /note="E -> K (in Ref. 2; BAB14613)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1023 AA;  110110 MW;  464408B227D764C8 CRC64;
     MCARMAGRTT AAPRGPYGPW LCLLVALALD VVRVDCGQAP LDPVYLPAAL ELLDAPEHFR
     VQQVGHYPPA NSSLSSRSET FLLLQPWPRA QPLLRASYPP FATQQVVPPR VTEPHQRPVP
     WDVRAVSVEA AVTPAEPYAR VLFHLKGQDW PPGSGSLPCA RLHATHPAGT AHQACRFQPS
     LGACVVELEL PSHWFSQAST TRAELAYTLE PAAEGPGGCG SGEENDPGEQ ALPVGGVELR
     PADPPQYQEV PLDEAVTLRV PDMPVRPGQL FSATLLLRHN FTASLLTLRI KVKKGLHVTA
     ARPAQPTLWT AKLDRFKGSR HHTTLITCHR AGLTEPDSSP LELSEFLWVD FVVENSTGGG
     VAVTRPVTWQ LEYPGQAPEA EKDKMVWEIL VSERDIRALI PLAKAEELVN TAPLTGVPQH
     VPVRLVTVDG GGALVEVTEH VGCESANTQV LQVSEACDAV FVAGKESRGA RGVRVDFWWR
     RLRASLRLTV WAPLLPLRIE LTDTTLEQVR GWRVPGPAEG PAEPAAEASD EAERRARGCH
     LQYQRAGVRF LAPFAAHPLD GGRRLTHLLG PDWLLDVSHL VAPHARVLDS RVASLEGGRV
     VVGREPGVTS IEVRSPLSDS ILGEQALAVT DDKVSVLELR VQPVMGISLT LSRGTAHPGE
     VTATCWAQSA LPAPKQEVAL SLWLSFSDHT VAPAELYDRR DLGLSVSAEE PGAILPAEEQ
     GAQLGVVVSG AGAEGLPLHV ALHPPEPCRR GRHRVPLASG TAWLGLPPAS TPAPALPSSP
     AWSPPATEAT MGGKRQVAGS VGGNTGVRGK FERAEEEARK EETEAREEEE EEEEEMVPAP
     QHVTELELGM YALLGVFCVA IFIFLVNGVV FVLRYQRKEP PDSATDPTSP QPHNWVWLGT
     DQEELSRQLD RQSPGPPKGE GSCPCESGGG GEAPTLAPGP PGGTTSSSST LARKEAGGRR
     KRVEFVTFAP APPAQSPEEP VGAPAVQSIL VAGEEDIRWV CEDMGLKDPE ELRNYMERIR
     GSS
//