ID KI26L_CAEEL Reviewed; 1066 AA. AC Q21441; Q9TVW0; Q9U541; DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2002, sequence version 3. DT 10-MAY-2017, entry version 123. DE RecName: Full=Kinesin-like protein vab-8; DE AltName: Full=Variable abnormal morphology protein 8; GN Name=vab-8; ORFNames=K12F2.2; OS Caenorhabditis elegans. OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; OC Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis. OX NCBI_TaxID=6239; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ALTERNATIVE SPLICING. RC STRAIN=Bristol N2; RA Hung M.-S., Way J.; RT "The Caenorhabditis elegans vab-8 gene encodes two kinesin-related RT proteins necessary for axon outgrowth and cell migration."; RL Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE RP SPLICING. RC STRAIN=Bristol N2; RX PubMed=9851916; DOI=10.1126/science.282.5396.2012; RG The C. elegans sequencing consortium; RT "Genome sequence of the nematode C. elegans: a platform for RT investigating biology."; RL Science 282:2012-2018(1998). RN [3] RP FUNCTION. RX PubMed=8625818; RA Wightman B., Clark S.G., Taskar A.M., Forrester W.C., Maricq A.V., RA Bargmann C.I., Garriga G.; RT "The C. elegans gene vab-8 guides posteriorly directed axon outgrowth RT and cell migration."; RL Development 122:671-682(1996). RN [4] RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY. RX PubMed=9581759; DOI=10.1016/S0896-6273(00)81006-5; RA Wolf F.W., Hung M.-S., Wightman B., Way J., Garriga G.; RT "vab-8 is a key regulator of posteriorly directed migrations in C. RT elegans and encodes a novel protein with kinesin motor similarity."; RL Neuron 20:655-666(1998). RN [5] RP FUNCTION, AND INTERACTION WITH UNC-51. RX PubMed=15539493; DOI=10.1242/dev.01457; RA Lai T., Garriga G.; RT "The conserved kinase UNC-51 acts with VAB-8 and UNC-14 to regulate RT axon outgrowth in C. elegans."; RL Development 131:5991-6000(2004). RN [6] RP FUNCTION. RX PubMed=17237777; DOI=10.1038/nn1835; RA Levy-Strumpf N., Culotti J.G.; RT "VAB-8, UNC-73 and MIG-2 regulate axon polarity and cell migration RT functions of UNC-40 in C. elegans."; RL Nat. Neurosci. 10:161-168(2007). RN [7] RP FUNCTION, AND INTERACTION WITH UNC-73. RX PubMed=17237778; DOI=10.1038/nn1834; RA Watari-Goshima N., Ogura K., Wolf F.W., Goshima Y., Garriga G.; RT "C. elegans VAB-8 and UNC-73 regulate the SAX-3 receptor to direct RT cell and growth-cone migrations."; RL Nat. Neurosci. 10:169-176(2007). CC -!- FUNCTION: Required for posterior migration of cells and axon CC growth cones during nervous system assembly. Isoform a is required CC for posterior migration of the axon growth cone and the ALM cell CC body. Isoform a may enhance posterior migration by regulating the CC subcellular location or stability of guidance cue receptors such CC as sax-3 and unc-40, promoting their localization to the cell CC surface. Isoform b is specifically required for CAN cell CC migration. {ECO:0000269|PubMed:15539493, CC ECO:0000269|PubMed:17237777, ECO:0000269|PubMed:17237778, CC ECO:0000269|PubMed:8625818, ECO:0000269|PubMed:9581759}. CC -!- SUBUNIT: Interacts with unc-51 and unc-73. CC {ECO:0000269|PubMed:15539493, ECO:0000269|PubMed:17237778}. CC -!- INTERACTION: CC Q23023:unc-51; NbExp=4; IntAct=EBI-2412191, EBI-329049; CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton CC {ECO:0000269|PubMed:9581759}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=a; Synonyms=vab-8l; CC IsoId=Q21441-1; Sequence=Displayed; CC Name=b; Synonyms=vab-8s; CC IsoId=Q21441-2; Sequence=VSP_028697, VSP_028698; CC -!- TISSUE SPECIFICITY: Isoform a is expressed in the AVKR neuron. CC Isoform b is expressed in the CAN cells and in the body wall CC muscle. Also expressed in the motor neurons of the ventral nerve CC cord and additional neurons in the pharynx and head. CC {ECO:0000269|PubMed:9581759}. CC -!- PTM: Phosphorylated by unc-51. CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase CC superfamily. Kinesin family. KIF26 subfamily. CC {ECO:0000255|PROSITE-ProRule:PRU00283}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AF108229; AAF17300.1; -; Genomic_DNA. DR EMBL; AF108229; AAF17301.1; -; Genomic_DNA. DR EMBL; Z78199; CAB01577.2; -; Genomic_DNA. DR EMBL; Z78201; CAB01577.2; JOINED; Genomic_DNA. DR EMBL; Z78199; CAB54275.1; -; Genomic_DNA. DR PIR; T23623; T23623. DR PIR; T23624; T23624. DR RefSeq; NP_506063.2; NM_073662.5. [Q21441-1] DR RefSeq; NP_506064.1; NM_073663.3. [Q21441-2] DR UniGene; Cel.6323; -. DR ProteinModelPortal; Q21441; -. DR IntAct; Q21441; 9. DR STRING; 6239.K12F2.2a; -. DR EPD; Q21441; -. DR PaxDb; Q21441; -. DR EnsemblMetazoa; K12F2.2a; K12F2.2a; WBGene00006874. [Q21441-1] DR GeneID; 179675; -. DR KEGG; cel:CELE_K12F2.2; -. DR UCSC; K12F2.2a; c. elegans. [Q21441-1] DR CTD; 179675; -. DR WormBase; K12F2.2a; CE31041; WBGene00006874; vab-8. [Q21441-1] DR WormBase; K12F2.2b; CE23870; WBGene00006874; vab-8. [Q21441-2] DR eggNOG; KOG4280; Eukaryota. DR eggNOG; COG5059; LUCA. DR GeneTree; ENSGT00650000093360; -. DR HOGENOM; HOG000113222; -. DR InParanoid; Q21441; -. DR OMA; DNENIAH; -. DR OrthoDB; EOG091G01WN; -. DR PRO; PR:Q21441; -. DR Proteomes; UP000001940; Chromosome V. DR Bgee; WBGene00006874; -. DR ExpressionAtlas; Q21441; baseline. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW. DR GO; GO:0005871; C:kinesin complex; IBA:GO_Central. DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0016887; F:ATPase activity; IBA:GO_Central. DR GO; GO:0008017; F:microtubule binding; IEA:InterPro. DR GO; GO:0003777; F:microtubule motor activity; IBA:GO_Central. DR GO; GO:0007411; P:axon guidance; IMP:WormBase. DR GO; GO:0016477; P:cell migration; IMP:WormBase. DR GO; GO:0040011; P:locomotion; IMP:WormBase. DR GO; GO:0008078; P:mesodermal cell migration; IMP:WormBase. DR GO; GO:0007018; P:microtubule-based movement; IBA:GO_Central. DR GO; GO:1905484; P:negative regulation of motor neuron migration; IMP:UniProtKB. DR GO; GO:0001764; P:neuron migration; IMP:WormBase. DR GO; GO:0018991; P:oviposition; IMP:WormBase. DR GO; GO:0030334; P:regulation of cell migration; IMP:WormBase. DR GO; GO:0040025; P:vulval development; IMP:WormBase. DR Gene3D; 3.40.850.10; -; 1. DR InterPro; IPR001752; Kinesin_motor_dom. DR InterPro; IPR027417; P-loop_NTPase. DR Pfam; PF00225; Kinesin; 1. DR SMART; SM00129; KISc; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR PROSITE; PS50067; KINESIN_MOTOR_2; 1. PE 1: Evidence at protein level; KW Alternative splicing; ATP-binding; Coiled coil; Complete proteome; KW Cytoplasm; Cytoskeleton; Developmental protein; Differentiation; KW Microtubule; Motor protein; Neurogenesis; Nucleotide-binding; KW Phosphoprotein; Reference proteome. FT CHAIN 1 1066 Kinesin-like protein vab-8. FT /FTId=PRO_0000307306. FT DOMAIN 15 326 Kinesin motor. {ECO:0000255|PROSITE- FT ProRule:PRU00283}. FT REGION 332 514 Interaction with unc-51. FT REGION 402 874 Interaction with unc-73. FT REGION 514 715 Interaction with unc-51. FT COILED 717 765 {ECO:0000255}. FT COILED 987 1021 {ECO:0000255}. FT VAR_SEQ 1 484 Missing (in isoform b). {ECO:0000305}. FT /FTId=VSP_028697. FT VAR_SEQ 485 513 FGGKTSEKREDFGIMIAQPSIPLMKAKSK -> MPQTSALF FT EIRRSQITFMEYFKYTWQRLM (in isoform b). FT {ECO:0000305}. FT /FTId=VSP_028698. SQ SEQUENCE 1066 AA; 118211 MW; A848740A95EC6EF0 CRC64; MEACSSKTSL LLHSPLRTIP KLRLCASISS EDVAHGRCSL TDQHLQIEGK NYSKTTFDHI FRTDATQDDM YTAFLSDTIN SVFAGNDATV LAMGAKTNGK DERLYGNSVS RNGLVQMAIT QLMNALDDNK DSEERIQVRM SAIMVSQNES SIVDLLSPFN PDPRHRVVKI VDDARTGVFI DNESEIRVET IDQALFYLNT AVDHRMIQDE HTHRTSHVFI SLSLYSYKMG DKMQGGRRRL CFLDMGIGER NSTNGGMTMP ALGSILLAMV QRNKHIPSRD SSVCQLIRCA LSTSRFTTFV FSFGAKSDDN ENIAHLACKI ARTRAKSMVG HGRKSSGTMS TGTMESNSSS CGTTTITPGG TPRTQRRFEL ESGSELSAAE TVIFLGPNSS RTASPASTTM PFTPTSIRPL HRTTRNHSGV EALSKPLSVE TKSSPTHNCH DGCIHSIPPM LRGHTPFLSA SLKLYDELCS PPSSSRASPA PPAAFGGKTS EKREDFGIMI AQPSIPLMKA KSKYNLDDGK MKQIMQWMET SEAPPILFSS PCYENSATSV EELRECVGIL SHPLEDIIEQ EEESMRTSTA TTGGSKKDHP LRILSKQDLN VEPEIKDKQE ENELELVMAA SLSSMRSHDI LAKLEAMRNA QNGNSVQNIG NSQSNTDMDV SEMDVYRRAS HLEEYAMQRV REIEENKLKN KKKIKLGLNC CQQQSMISSG STVVDWSQIE RKKEKEREVH EEEMRKEMLR ERRAKLKITE LEIKRERNLI DKELDDKKGI ANSIARQLQH FSLSPCRGGR THRSVSTHRI DPPNASLPST PTMSHKKVIG GSLAKLSASG ASGSTGAPPS PALGYHQSLP RHSKLPTSVN GRRASAERER KSNKASRNSC SKERKISGSK EELQWRSPYA QMTSPKSYGG PGTSSSGRGS SAPGSDFETP VVSTTEKSAN GTIPRSKRQS YSASSGYESA NDYHIYSTTN KKPHILDKKR NEEKLSLVRQ ADEIRHRQWQ LKKELEEAKR AIGQEDDAKM IANSSDQRLN GLSRTTMIDA MLQENRILEK RLVACRNHSM LVTTFI //