ID YJEN3_DANRE Reviewed; 246 AA. AC Q1LVI2; DT 12-AUG-2020, integrated into UniProtKB/Swiss-Prot. DT 30-MAY-2006, sequence version 1. DT 03-AUG-2022, entry version 94. DE RecName: Full=YjeF N-terminal domain-containing 3; DE AltName: Full=ApoA-I-binding protein 2 {ECO:0000303|PubMed:30705153}; GN Name=yjefn3 {ECO:0000312|ZFIN:ZDB-GENE-050208-755}; GN Synonyms=aibp2 {ECO:0000303|PubMed:30705153}; OS Danio rerio (Zebrafish) (Brachydanio rerio). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Danionidae; Danioninae; Danio. OX NCBI_TaxID=7955; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Tuebingen; RX PubMed=23594743; DOI=10.1038/nature12111; RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J., RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G., RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P., RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.; RT "The zebrafish reference genome sequence and its relationship to the human RT genome."; RL Nature 496:498-503(2013). RN [2] RP FUNCTION, DEVELOPMENTAL STAGE, INTERACTION WITH APOA1, BINDS TO HDL, AND RP DISRUPTION PHENOTYPE. RX PubMed=23719382; DOI=10.1038/nature12166; RA Fang L., Choi S.H., Baek J.S., Liu C., Almazan F., Ulrich F., Wiesner P., RA Taleb A., Deer E., Pattison J., Torres-Vazquez J., Li A.C., Miller Y.I.; RT "Control of angiogenesis by AIBP-mediated cholesterol efflux."; RL Nature 498:118-122(2013). RN [3] RP FUNCTION, AND DISRUPTION PHENOTYPE. RX PubMed=30705153; DOI=10.1126/science.aav1749; RA Gu Q., Yang X., Lv J., Zhang J., Xia B., Kim J.D., Wang R., Xiong F., RA Meng S., Clements T.P., Tandon B., Wagner D.S., Diaz M.F., Wenzel P.L., RA Miller Y.I., Traver D., Cooke J.P., Li W., Zon L.I., Chen K., Bai Y., RA Fang L.; RT "AIBP-mediated cholesterol efflux instructs hematopoietic stem and RT progenitor cell fate."; RL Science 363:1085-1088(2019). CC -!- FUNCTION: Accelerates cholesterol efflux from endothelial cells to CC high-density lipoprotein (HDL) and thereby regulates angiogenesis CC (PubMed:23719382). Orchestrates hematopoietic stem and progenitor cell CC emergence from the hemogenic endothelium, a type of specialized CC endothelium manifesting hematopoietic potential. YJEFN3-mediated CC cholesterol efflux activates endothelial SREBF2, the master CC transcription factor for cholesterol biosynthesis, which in turn CC transactivates NOTCH and promotes hematopoietic stem and progenitor CC cell emergence (PubMed:30705153). {ECO:0000269|PubMed:23719382, CC ECO:0000269|PubMed:30705153}. CC -!- SUBUNIT: Interacts with APOA1 (PubMed:23719382). Binds to HDL CC (PubMed:23719382). {ECO:0000269|PubMed:23719382}. CC -!- DEVELOPMENTAL STAGE: Expression in 24-36 hours post-fertilization (hpf) CC embryos shows a clear segmental pattern. By 48 hpf, when segmental CC angiogenesis is completed, is no longer expressed in somites. CC {ECO:0000269|PubMed:23719382}. CC -!- DISRUPTION PHENOTYPE: Mutants appear morphologically normal and have no CC observable effect on dorsal aorta specification (PubMed:30705153). CC Mutants have reduced the number of nascent hematopoietic stem cells in CC the ventral dorsal aorta between 28 and 60 hours post-fertilization CC (hpf) (PubMed:30705153). Mutant embryos show increased cholesterol CC content (PubMed:30705153, PubMed:23719382). CC {ECO:0000269|PubMed:23719382, ECO:0000269|PubMed:30705153}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; BX649279; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR EMBL; BX664610; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR RefSeq; NP_001038308.1; NM_001044843.1. DR AlphaFoldDB; Q1LVI2; -. DR SMR; Q1LVI2; -. DR STRING; 7955.ENSDARP00000082703; -. DR PaxDb; Q1LVI2; -. DR Ensembl; ENSDART00000088270; ENSDARP00000082703; ENSDARG00000061692. DR GeneID; 557840; -. DR KEGG; dre:557840; -. DR CTD; 374887; -. DR ZFIN; ZDB-GENE-050208-755; yjefn3. DR eggNOG; KOG2585; Eukaryota. DR GeneTree; ENSGT00390000007227; -. DR HOGENOM; CLU_024853_3_0_1; -. DR InParanoid; Q1LVI2; -. DR OMA; NIKEPYP; -. DR OrthoDB; 1030667at2759; -. DR PhylomeDB; Q1LVI2; -. DR TreeFam; TF300197; -. DR PRO; PR:Q1LVI2; -. DR Proteomes; UP000000437; Genome assembly. DR Proteomes; UP000814640; Chromosome 22. DR Bgee; ENSDARG00000061692; Expressed in brain and 12 other tissues. DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central. DR GO; GO:0052856; F:NADHX epimerase activity; IBA:GO_Central. DR GO; GO:0071425; P:hematopoietic stem cell proliferation; IMP:UniProtKB. DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW. DR GO; GO:0031580; P:membrane raft distribution; IMP:ZFIN. DR GO; GO:0016525; P:negative regulation of angiogenesis; IMP:ZFIN. DR GO; GO:0010874; P:regulation of cholesterol efflux; IMP:UniProtKB. DR GO; GO:0008593; P:regulation of Notch signaling pathway; IMP:UniProtKB. DR GO; GO:0002040; P:sprouting angiogenesis; IMP:ZFIN. DR Gene3D; 3.40.50.10260; -; 1. DR InterPro; IPR004443; YjeF_N_dom. DR InterPro; IPR036652; YjeF_N_dom_sf. DR InterPro; IPR032976; YJEFN_prot_NAXE-like. DR PANTHER; PTHR13232; PTHR13232; 1. DR Pfam; PF03853; YjeF_N; 1. DR SUPFAM; SSF64153; SSF64153; 1. DR TIGRFAMs; TIGR00197; yjeF_nterm; 1. DR PROSITE; PS51385; YJEF_N; 1. PE 1: Evidence at protein level; KW Lipid transport; Reference proteome; Transport. FT CHAIN 1..246 FT /note="YjeF N-terminal domain-containing 3" FT /id="PRO_0000450455" FT DOMAIN 24..234 FT /note="YjeF N-terminal" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00719" SQ SEQUENCE 246 AA; 27609 MW; AD6F399AAB25EE3D CRC64; MNHSSNEKEP ETIEPLRYLS KTEVATVETE LLRDYRFGQQ QLIEIWGHAC AIAITKAFPL SSLSKKQPTL LVVCGPEQNG SIGLVCARHL RMFEYEPTIF YPKRSTLGLH QDFTVQCEKM DIPFLSYLPT EVQLLNDAYN LVIDAILGPE TDHKDVKEPY AGMLVTLKQV KIPIVSVDVP SGWDADEPAK DGINPEVLIS LTAPKKCATG FSGKHFLAGR FLPYDIQKKY ELNLPEFPGT ECIIEL //