ID Q1K9G5_INBAA Unreviewed; 752 AA. AC Q1K9G5; DT 30-MAY-2006, integrated into UniProtKB/TrEMBL. DT 30-MAY-2006, sequence version 1. DT 11-DEC-2019, entry version 54. DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|HAMAP-Rule:MF_04065}; DE EC=2.7.7.48 {ECO:0000256|HAMAP-Rule:MF_04065}; DE AltName: Full=Polymerase basic protein 1 {ECO:0000256|HAMAP-Rule:MF_04065}; DE Short=PB1 {ECO:0000256|HAMAP-Rule:MF_04065}; DE AltName: Full=RNA-directed RNA polymerase subunit P1 {ECO:0000256|HAMAP-Rule:MF_04065}; GN Name=PB1 {ECO:0000256|HAMAP-Rule:MF_04065, GN ECO:0000313|EMBL:ABF21251.1}; OS Influenza B virus (strain B/Ann Arbor/1/1986). OC Viruses; Riboviria; Negarnaviricota; Polyploviricotina; Insthoviricetes; OC Articulavirales; Orthomyxoviridae; Betainfluenzavirus. OX NCBI_TaxID=11521 {ECO:0000313|EMBL:ABF21251.1, ECO:0000313|Proteomes:UP000160484}; OH NCBI_TaxID=9606; Homo sapiens (Human). RN [1] {ECO:0000313|EMBL:ABF21251.1, ECO:0000313|Proteomes:UP000160484} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=B/Ann Arbor/1/1986 {ECO:0000313|EMBL:ABF21251.1}; RA Mbawuike I.N., Zhang Y., Yamada R.E., Nino D., Bui H.-H., Sette A., RA Couch R.B.; RT "Complete Genome Sequencing And Analysis Of Selected Influenza Virus RT Vaccine Strains Spanning Six Decades (1933-1999)."; RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for CC replication and transcription of virus RNA segments. The transcription CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides CC by PA. In turn, these short capped RNAs are used as primers by PB1 for CC transcription of viral mRNAs. During virus replication, PB1 initiates CC RNA synthesis and copies vRNA into complementary RNA (cRNA) which in CC turn serves as a template for the production of more vRNAs. CC {ECO:0000256|HAMAP-Rule:MF_04065}. CC -!- CATALYTIC ACTIVITY: CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:11128, Rhea:RHEA- CC COMP:11129, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:83400; CC EC=2.7.7.48; Evidence={ECO:0000256|HAMAP-Rule:MF_04065, CC ECO:0000256|RuleBase:RU004330, ECO:0000256|SAAS:SAAS01176592}; CC -!- SUBUNIT: Influenza RNA polymerase is composed of three subunits: PB1, CC PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). CC Interacts (via C-terminus) with PB2 (via N-terminus); this interaction CC is essential for transcription initiation. {ECO:0000256|HAMAP- CC Rule:MF_04065}. CC -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000256|HAMAP-Rule:MF_04065}. CC Host cytoplasm {ECO:0000256|HAMAP-Rule:MF_04065}. CC -!- PTM: Phosphorylated by host PRKCA. {ECO:0000256|HAMAP-Rule:MF_04065}. CC -!- SIMILARITY: Belongs to the influenza viruses polymerase PB1 family. CC {ECO:0000256|HAMAP-Rule:MF_04065, ECO:0000256|SAAS:SAAS00997533}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; DQ508911; ABF21251.1; -; Genomic_RNA. DR Proteomes; UP000160484; Genome. DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-UniRule. DR GO; GO:0003723; F:RNA binding; IEA:InterPro. DR GO; GO:0003968; F:RNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule. DR GO; GO:0039523; P:suppression by virus of host RNA polymerase II activity; IEA:UniProtKB-UniRule. DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule. DR GO; GO:0039694; P:viral RNA genome replication; IEA:UniProtKB-UniRule. DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW. DR HAMAP; MF_04065; INFV_RDRP; 1. DR InterPro; IPR007099; RNA-dir_pol_NSvirus. DR InterPro; IPR001407; RNA_pol_PB1_influenza. DR Pfam; PF00602; Flu_PB1; 1. DR PIRSF; PIRSF000827; RdRPol_OMV; 1. DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1. PE 3: Inferred from homology; KW Eukaryotic host gene expression shutoff by virus {ECO:0000256|HAMAP- KW Rule:MF_04065}; KW Eukaryotic host transcription shutoff by virus {ECO:0000256|HAMAP- KW Rule:MF_04065}; Host cytoplasm {ECO:0000256|HAMAP-Rule:MF_04065}; KW Host gene expression shutoff by virus {ECO:0000256|HAMAP-Rule:MF_04065}; KW Host nucleus {ECO:0000256|HAMAP-Rule:MF_04065}; KW Host-virus interaction {ECO:0000256|HAMAP-Rule:MF_04065}; KW Inhibition of host RNA polymerase II by virus {ECO:0000256|HAMAP- KW Rule:MF_04065}; KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_04065, KW ECO:0000256|SAAS:SAAS01176568}; KW Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_04065, KW ECO:0000256|SAAS:SAAS01176569}; KW Phosphoprotein {ECO:0000256|HAMAP-Rule:MF_04065}; KW RNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_04065, KW ECO:0000256|RuleBase:RU004330, ECO:0000256|SAAS:SAAS01176590}; KW Transferase {ECO:0000256|HAMAP-Rule:MF_04065, KW ECO:0000256|SAAS:SAAS01176594}; KW Viral RNA replication {ECO:0000256|HAMAP-Rule:MF_04065, KW ECO:0000256|SAAS:SAAS01176589}; KW Viral transcription {ECO:0000256|HAMAP-Rule:MF_04065}. FT DOMAIN 286..482 FT /note="RdRp catalytic" FT /evidence="ECO:0000259|PROSITE:PS50525" FT REGION 249..256 FT /note="Promoter-binding site" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04065" FT MOTIF 187..195 FT /note="Nuclear localization signal" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04065" FT MOTIF 203..216 FT /note="Nuclear localization signal" FT /evidence="ECO:0000256|HAMAP-Rule:MF_04065" SQ SEQUENCE 752 AA; 84351 MW; B136FCBB96BB640A CRC64; MNINPYFLFI DVPIQAAIST TFPYTGVPPY SHGTGTGYTI DTVIRTHEYS NKGKQYISDV TGCTMIDPTN GPLPEDNEPS AYAQLDCVLE ALDRMDEEHP GLFQAASQNA MEALMVTTVD KLTQGRQTFD WTVCRNQPAA TALNTTITSF RLNDLNGADK GGLVPFCQDI IDSLDRPEMT FFSVKNIKKK LPAKNRKGFL IKRIPMKVKD RITRVEYIKR ALSLNTMTKD AERGKLKRRA IATAGIQIRG FVLVVENLAK NICENLEQSG LPVGGNEKKA KLSNAVAKML SNCPPGGISM TVTGDNTKWN ECLNPRIFLA MTERITRDSP IWFRDFCSIA PVLFSNKIAR LGKGFMITSK TKRLKAQIPC PDLFSIPLER YNEETRAKLK KLKPFFNEEG TASLSPGMMM GMFNMLSTVL GVAALGIKNI GNKEYLWDGL QSSDDFALFV NAKDEETCME GINDFYRTCK LLGINMSKKK SYCNETGMFE FTSMFYRDGF VSNFAMELPS FGVAGVNESA DMAIGMTIIK NNMINNGMGP ATAQTAIQLF IADYRYTYKC HRGDSKVEGK RMKIIKELWE NTKGRDGLLV ADGGPNIYNL RNLHIPEIVL KYNLMDPEYK GRLLHPQNPF VGHLSIEGIK EADITPAHGP VKKMDYDAVS GTHSWRTKRN RSILNTDQRN MILEEQCYAK CCNLFEACFN SASYRKPVGQ HSMLEAMAHR LRMDARLDYE SGRMSKDDFE KAMAHLGEIG YI //