ID WRN_CAEEL Reviewed; 1056 AA. AC Q19546; DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2001, sequence version 2. DT 16-OCT-2019, entry version 145. DE RecName: Full=Probable Werner syndrome ATP-dependent helicase homolog 1; DE EC=3.6.4.12; GN Name=wrn-1; ORFNames=F18C5.2; OS Caenorhabditis elegans. OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; OC Caenorhabditis. OX NCBI_TaxID=6239; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Bristol N2; RX PubMed=9851916; DOI=10.1126/science.282.5396.2012; RG The C. elegans sequencing consortium; RT "Genome sequence of the nematode C. elegans: a platform for RT investigating biology."; RL Science 282:2012-2018(1998). RN [2] {ECO:0000305} RP REPEATS. RX PubMed=10049920; RA Kusano K., Berres M.E., Engels W.R.; RT "Evolution of the RECQ family of helicases: a Drosophila homolog, RT Dmblm, is similar to the human Bloom syndrome gene."; RL Genetics 151:1027-1039(1999). CC -!- FUNCTION: Essential for the formation of DNA replication focal CC centers; stably associates with foci elements generating binding CC sites for RP-A. Exhibits a magnesium-dependent ATP-dependent DNA- CC helicase activity. May be involved in the control of genomic CC stability (By similarity). {ECO:0000250}. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. CC -!- SIMILARITY: Belongs to the helicase family. RecQ subfamily. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; FO080970; CCD68193.1; -; Genomic_DNA. DR PIR; T16087; T16087. DR RefSeq; NP_495324.2; NM_062923.5. DR SMR; Q19546; -. DR BioGrid; 39420; 13. DR IntAct; Q19546; 4. DR MINT; Q19546; -. DR STRING; 6239.F18C5.2; -. DR EPD; Q19546; -. DR PaxDb; Q19546; -. DR PeptideAtlas; Q19546; -. DR PRIDE; Q19546; -. DR EnsemblMetazoa; F18C5.2.1; F18C5.2.1; WBGene00006944. DR GeneID; 174081; -. DR KEGG; cel:CELE_F18C5.2; -. DR UCSC; F18C5.2; c. elegans. DR CTD; 174081; -. DR WormBase; F18C5.2; CE31791; WBGene00006944; wrn-1. DR eggNOG; KOG0351; Eukaryota. DR eggNOG; COG0514; LUCA. DR GeneTree; ENSGT00940000159168; -. DR HOGENOM; HOG000016918; -. DR InParanoid; Q19546; -. DR KO; K10900; -. DR OMA; NCVIMAT; -. DR OrthoDB; 445763at2759; -. DR PhylomeDB; Q19546; -. DR BRENDA; 3.6.4.12; 1045. DR Reactome; R-CEL-5685938; HDR through Single Strand Annealing (SSA). DR Reactome; R-CEL-5693607; Processing of DNA double-strand break ends. DR PRO; PR:Q19546; -. DR Proteomes; UP000001940; Chromosome II. DR Bgee; WBGene00006944; Expressed in 5 organ(s), highest expression level in adult organism. DR GO; GO:0005694; C:chromosome; IDA:WormBase. DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. DR GO; GO:0005654; C:nucleoplasm; IDA:WormBase. DR GO; GO:0005634; C:nucleus; IDA:WormBase. DR GO; GO:0035861; C:site of double-strand break; IDA:WormBase. DR GO; GO:0043138; F:3'-5' DNA helicase activity; IDA:WormBase. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW. DR GO; GO:0009378; F:four-way junction helicase activity; IBA:GO_Central. DR GO; GO:0008340; P:determination of adult lifespan; IMP:WormBase. DR GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central. DR GO; GO:0006259; P:DNA metabolic process; IDA:WormBase. DR GO; GO:0006310; P:DNA recombination; IBA:GO_Central. DR GO; GO:0006281; P:DNA repair; IBA:GO_Central. DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central. DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central. DR GO; GO:0044806; P:G-quadruplex DNA unwinding; IBA:GO_Central. DR GO; GO:0010259; P:multicellular organism aging; IBA:GO_Central. DR GO; GO:0010212; P:response to ionizing radiation; IMP:WormBase. DR GO; GO:0000723; P:telomere maintenance; IBA:GO_Central. DR Gene3D; 1.10.10.10; -; 1. DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom. DR InterPro; IPR004589; DNA_helicase_ATP-dep_RecQ. DR InterPro; IPR014001; Helicase_ATP-bd. DR InterPro; IPR001650; Helicase_C. DR InterPro; IPR029491; Helicase_HTH. DR InterPro; IPR010997; HRDC-like_sf. DR InterPro; IPR002121; HRDC_dom. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR032284; RecQ_Zn-bd. DR InterPro; IPR018982; RQC_domain. DR InterPro; IPR036388; WH-like_DNA-bd_sf. DR InterPro; IPR036390; WH_DNA-bd_sf. DR Pfam; PF00270; DEAD; 1. DR Pfam; PF00271; Helicase_C; 1. DR Pfam; PF00570; HRDC; 1. DR Pfam; PF14493; HTH_40; 1. DR Pfam; PF16124; RecQ_Zn_bind; 1. DR Pfam; PF09382; RQC; 1. DR SMART; SM00487; DEXDc; 1. DR SMART; SM00490; HELICc; 1. DR SMART; SM00341; HRDC; 1. DR SMART; SM00956; RQC; 1. DR SUPFAM; SSF46785; SSF46785; 1. DR SUPFAM; SSF47819; SSF47819; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR TIGRFAMs; TIGR00614; recQ_fam; 1. DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1. DR PROSITE; PS51194; HELICASE_CTER; 1. DR PROSITE; PS50967; HRDC; 1. PE 3: Inferred from homology; KW ATP-binding; Complete proteome; DNA-binding; Helicase; Hydrolase; KW Nucleotide-binding; Nucleus; Reference proteome; Repeat. FT CHAIN 1 1056 Probable Werner syndrome ATP-dependent FT helicase homolog 1. FT /FTId=PRO_0000205048. FT REPEAT 17 26 1. {ECO:0000269|PubMed:10049920}. FT REPEAT 28 37 2. {ECO:0000269|PubMed:10049920}. FT DOMAIN 236 406 Helicase ATP-binding. FT {ECO:0000255|PROSITE-ProRule:PRU00541}. FT DOMAIN 427 583 Helicase C-terminal. FT {ECO:0000255|PROSITE-ProRule:PRU00542}. FT DOMAIN 806 886 HRDC. {ECO:0000255|PROSITE- FT ProRule:PRU00328, ECO:0000305}. FT NP_BIND 249 256 ATP. {ECO:0000255|PROSITE- FT ProRule:PRU00541}. FT REGION 17 37 2 X 10 AA repeats of N-[ED]-E-L-P-E-T-E- FT P-E. FT MOTIF 348 351 DEAH box. FT COMPBIAS 4 7 Poly-Asp. FT COMPBIAS 90 95 Poly-Ser. FT COMPBIAS 628 631 Poly-Ser. SQ SEQUENCE 1056 AA; 118523 MW; 99C23E70D06DAA0B CRC64; MISDDDDLPS TRPGSVNEEL PETEPEDNDE LPETEPESDS DKPTVTSNKT ENQVADEDYD SFDDFVPSQT HTASKIPVKN KRAKKCTVES DSSSSDDSDQ GDDCEFIPAC DETQEVPKIK RGYTLRTRAS VKNKCDDSWD DGIDEEDVSK RSEDTLNDSF VDPEFMDSVL DNQLTIKGKK QFLDDGEFFT DRNVPQIDEA TKMKWASMTS PPQEALNALN EFFGHKGFRE KQWDVVRNVL GGKDQFVLMS TGYGKSVCYQ LPSLLLNSMT VVVSPLISLM NDQVTTLVSK GIDAVKLDGH STQIEWDQVA NNMHRIRFIY MSPEMVTSQK GLELLTSCRK HISLLAIDEA HCVSQWGHDF RNSYRHLAEI RNRSDLCNIP MIALTATATV RVRDDVIANL RLRKPLITTT SFDRKNLYIS VHSSKDMAED LGLFMKTDEV KGRHFGGPTI IYCQTKQMVD DVNCVLRRIG VRSAHYHAGL TKNQREKAHT DFMRDKITTI VATVAFGMGI DKPDVRNVIH YGCPNNIESY YQEIGRAGRD GSPSICRVFW APKDLNTIKF KLRNSQQKEE VVENLTMMLR QLELVLTTVG CRRYQLLKHF DPSYAKPPTM QADCCDRCTE MLNGNQDSSS SIVDVTTESK WLFQVINEMY NGKTGIGKPI EFLRGSSKED WRIKTTSQQK LFGIGKHIPD KWWKALAASL RIAGYLGEVR LMQMKFGSCI TLSELGERWL LTGKEMKIDA TPILLQGKKE KAAPSTVPGA SRSQSTKSST EIPTKILGAN KIREYEPANE NEQLMNLKKQ EVTGLPEKID QLRSRLDDIR VGIANMHEVA PFQIVSNTVL DCFANLRPTS ASNLEMIDGM SAQQKSRYGK RFVDCVVQFS KETGIATNVN ANDMIPPELI SKMQKVLSDA VRRVYTEHLI SRSTAKEVAT ARGISEGTVY SYLAMAVEKG LPLHLDKLNV SRKNIAMALN AVRVHLGSNV AVLTPWVEAM GVVPDFNQLK LIRAILIYEY GLDTSENQEK PDIQSMPSTS NPSTIKTVPS TPSSSLRAPP LKKFKL //