ID WRN_CAEEL Reviewed; 1056 AA. AC Q19546; DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-2001, sequence version 2. DT 05-DEC-2018, entry version 139. DE RecName: Full=Probable Werner syndrome ATP-dependent helicase homolog 1; DE EC=3.6.4.12; GN Name=wrn-1; ORFNames=F18C5.2; OS Caenorhabditis elegans. OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; OC Caenorhabditis. OX NCBI_TaxID=6239; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Bristol N2; RX PubMed=9851916; DOI=10.1126/science.282.5396.2012; RG The C. elegans sequencing consortium; RT "Genome sequence of the nematode C. elegans: a platform for RT investigating biology."; RL Science 282:2012-2018(1998). RN [2] {ECO:0000305} RP REPEATS. RX PubMed=10049920; RA Kusano K., Berres M.E., Engels W.R.; RT "Evolution of the RECQ family of helicases: a Drosophila homolog, RT Dmblm, is similar to the human Bloom syndrome gene."; RL Genetics 151:1027-1039(1999). CC -!- FUNCTION: Essential for the formation of DNA replication focal CC centers; stably associates with foci elements generating binding CC sites for RP-A. Exhibits a magnesium-dependent ATP-dependent DNA- CC helicase activity. May be involved in the control of genomic CC stability (By similarity). {ECO:0000250}. CC -!- CATALYTIC ACTIVITY: CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. CC -!- SIMILARITY: Belongs to the helicase family. RecQ subfamily. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; FO080970; CCD68193.1; -; Genomic_DNA. DR PIR; T16087; T16087. DR RefSeq; NP_495324.2; NM_062923.5. DR UniGene; Cel.7245; -. DR ProteinModelPortal; Q19546; -. DR SMR; Q19546; -. DR BioGrid; 39420; 5. DR IntAct; Q19546; 4. DR MINT; Q19546; -. DR STRING; 6239.F18C5.2; -. DR EPD; Q19546; -. DR PaxDb; Q19546; -. DR PeptideAtlas; Q19546; -. DR PRIDE; Q19546; -. DR EnsemblMetazoa; F18C5.2; F18C5.2; WBGene00006944. DR GeneID; 174081; -. DR KEGG; cel:CELE_F18C5.2; -. DR UCSC; F18C5.2; c. elegans. DR CTD; 174081; -. DR WormBase; F18C5.2; CE31791; WBGene00006944; wrn-1. DR eggNOG; KOG0351; Eukaryota. DR eggNOG; COG0514; LUCA. DR GeneTree; ENSGT00940000159168; -. DR HOGENOM; HOG000016918; -. DR InParanoid; Q19546; -. DR KO; K10900; -. DR OMA; NCVIMAT; -. DR OrthoDB; EOG091G0B07; -. DR PhylomeDB; Q19546; -. DR BRENDA; 3.6.4.12; 1045. DR PRO; PR:Q19546; -. DR Proteomes; UP000001940; Chromosome II. DR Bgee; WBGene00006944; Expressed in 5 organ(s), highest expression level in adult organism. DR GO; GO:0005813; C:centrosome; IBA:GO_Central. DR GO; GO:0005694; C:chromosome; IDA:WormBase. DR GO; GO:0000781; C:chromosome, telomeric region; IBA:GO_Central. DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central. DR GO; GO:0005654; C:nucleoplasm; IDA:WormBase. DR GO; GO:0005634; C:nucleus; IDA:WormBase. DR GO; GO:0035861; C:site of double-strand break; IDA:WormBase. DR GO; GO:0008408; F:3'-5' exonuclease activity; IBA:GO_Central. DR GO; GO:0070337; F:3'-flap-structured DNA binding; IBA:GO_Central. DR GO; GO:1905773; F:8-hydroxy-2'-deoxyguanosine DNA binding; IBA:GO_Central. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0043140; F:ATP-dependent 3'-5' DNA helicase activity; IDA:WormBase. DR GO; GO:0000405; F:bubble DNA binding; IBA:GO_Central. DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central. DR GO; GO:0003677; F:DNA binding; IBA:GO_Central. DR GO; GO:0061749; F:forked DNA-dependent helicase activity; IBA:GO_Central. DR GO; GO:0000400; F:four-way junction DNA binding; IBA:GO_Central. DR GO; GO:0009378; F:four-way junction helicase activity; IBA:GO_Central. DR GO; GO:0000287; F:magnesium ion binding; IBA:GO_Central. DR GO; GO:0030145; F:manganese ion binding; IBA:GO_Central. DR GO; GO:0042803; F:protein homodimerization activity; IBA:GO_Central. DR GO; GO:0044877; F:protein-containing complex binding; IBA:GO_Central. DR GO; GO:0061821; F:telomeric D-loop binding; IBA:GO_Central. DR GO; GO:0000403; F:Y-form DNA binding; IBA:GO_Central. DR GO; GO:0006284; P:base-excision repair; IBA:GO_Central. DR GO; GO:0007569; P:cell aging; IBA:GO_Central. DR GO; GO:0071480; P:cellular response to gamma radiation; IBA:GO_Central. DR GO; GO:0009267; P:cellular response to starvation; IBA:GO_Central. DR GO; GO:0008340; P:determination of adult lifespan; IMP:WormBase. DR GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central. DR GO; GO:0006259; P:DNA metabolic process; IDA:WormBase. DR GO; GO:0006310; P:DNA recombination; IBA:GO_Central. DR GO; GO:0006281; P:DNA repair; IBA:GO_Central. DR GO; GO:0000731; P:DNA synthesis involved in DNA repair; IBA:GO_Central. DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IBA:GO_Central. DR GO; GO:0044806; P:G-quadruplex DNA unwinding; IBA:GO_Central. DR GO; GO:0010259; P:multicellular organism aging; IBA:GO_Central. DR GO; GO:0051345; P:positive regulation of hydrolase activity; IBA:GO_Central. DR GO; GO:0098530; P:positive regulation of strand invasion; IBA:GO_Central. DR GO; GO:0042981; P:regulation of apoptotic process; IBA:GO_Central. DR GO; GO:0031297; P:replication fork processing; IBA:GO_Central. DR GO; GO:0001302; P:replicative cell aging; IBA:GO_Central. DR GO; GO:0010212; P:response to ionizing radiation; IMP:WormBase. DR GO; GO:0006979; P:response to oxidative stress; IBA:GO_Central. DR GO; GO:0010225; P:response to UV-C; IBA:GO_Central. DR GO; GO:0061820; P:telomeric D-loop disassembly; IBA:GO_Central. DR CDD; cd00079; HELICc; 1. DR Gene3D; 1.10.10.10; -; 1. DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom. DR InterPro; IPR004589; DNA_helicase_ATP-dep_RecQ. DR InterPro; IPR014001; Helicase_ATP-bd. DR InterPro; IPR001650; Helicase_C. DR InterPro; IPR029491; Helicase_HTH. DR InterPro; IPR010997; HRDC-like_sf. DR InterPro; IPR002121; HRDC_dom. DR InterPro; IPR027417; P-loop_NTPase. DR InterPro; IPR032284; RecQ_Zn-bd. DR InterPro; IPR018982; RQC_domain. DR InterPro; IPR036388; WH-like_DNA-bd_sf. DR InterPro; IPR036390; WH_DNA-bd_sf. DR Pfam; PF00270; DEAD; 1. DR Pfam; PF00271; Helicase_C; 1. DR Pfam; PF00570; HRDC; 1. DR Pfam; PF14493; HTH_40; 1. DR Pfam; PF16124; RecQ_Zn_bind; 1. DR Pfam; PF09382; RQC; 1. DR SMART; SM00487; DEXDc; 1. DR SMART; SM00490; HELICc; 1. DR SMART; SM00341; HRDC; 1. DR SMART; SM00956; RQC; 1. DR SUPFAM; SSF46785; SSF46785; 1. DR SUPFAM; SSF47819; SSF47819; 1. DR SUPFAM; SSF52540; SSF52540; 1. DR TIGRFAMs; TIGR00614; recQ_fam; 1. DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1. DR PROSITE; PS51194; HELICASE_CTER; 1. DR PROSITE; PS50967; HRDC; 1. PE 3: Inferred from homology; KW ATP-binding; Complete proteome; DNA-binding; Helicase; Hydrolase; KW Nucleotide-binding; Nucleus; Reference proteome; Repeat. FT CHAIN 1 1056 Probable Werner syndrome ATP-dependent FT helicase homolog 1. FT /FTId=PRO_0000205048. FT REPEAT 17 26 1. {ECO:0000269|PubMed:10049920}. FT REPEAT 28 37 2. {ECO:0000269|PubMed:10049920}. FT DOMAIN 236 406 Helicase ATP-binding. FT {ECO:0000255|PROSITE-ProRule:PRU00541}. FT DOMAIN 427 583 Helicase C-terminal. FT {ECO:0000255|PROSITE-ProRule:PRU00542}. FT DOMAIN 806 886 HRDC. {ECO:0000255|PROSITE- FT ProRule:PRU00328, ECO:0000305}. FT NP_BIND 249 256 ATP. {ECO:0000255|PROSITE- FT ProRule:PRU00541}. FT REGION 17 37 2 X 10 AA repeats of N-[ED]-E-L-P-E-T-E- FT P-E. FT MOTIF 348 351 DEAH box. FT COMPBIAS 4 7 Poly-Asp. FT COMPBIAS 90 95 Poly-Ser. FT COMPBIAS 628 631 Poly-Ser. SQ SEQUENCE 1056 AA; 118523 MW; 99C23E70D06DAA0B CRC64; MISDDDDLPS TRPGSVNEEL PETEPEDNDE LPETEPESDS DKPTVTSNKT ENQVADEDYD SFDDFVPSQT HTASKIPVKN KRAKKCTVES DSSSSDDSDQ GDDCEFIPAC DETQEVPKIK RGYTLRTRAS VKNKCDDSWD DGIDEEDVSK RSEDTLNDSF VDPEFMDSVL DNQLTIKGKK QFLDDGEFFT DRNVPQIDEA TKMKWASMTS PPQEALNALN EFFGHKGFRE KQWDVVRNVL GGKDQFVLMS TGYGKSVCYQ LPSLLLNSMT VVVSPLISLM NDQVTTLVSK GIDAVKLDGH STQIEWDQVA NNMHRIRFIY MSPEMVTSQK GLELLTSCRK HISLLAIDEA HCVSQWGHDF RNSYRHLAEI RNRSDLCNIP MIALTATATV RVRDDVIANL RLRKPLITTT SFDRKNLYIS VHSSKDMAED LGLFMKTDEV KGRHFGGPTI IYCQTKQMVD DVNCVLRRIG VRSAHYHAGL TKNQREKAHT DFMRDKITTI VATVAFGMGI DKPDVRNVIH YGCPNNIESY YQEIGRAGRD GSPSICRVFW APKDLNTIKF KLRNSQQKEE VVENLTMMLR QLELVLTTVG CRRYQLLKHF DPSYAKPPTM QADCCDRCTE MLNGNQDSSS SIVDVTTESK WLFQVINEMY NGKTGIGKPI EFLRGSSKED WRIKTTSQQK LFGIGKHIPD KWWKALAASL RIAGYLGEVR LMQMKFGSCI TLSELGERWL LTGKEMKIDA TPILLQGKKE KAAPSTVPGA SRSQSTKSST EIPTKILGAN KIREYEPANE NEQLMNLKKQ EVTGLPEKID QLRSRLDDIR VGIANMHEVA PFQIVSNTVL DCFANLRPTS ASNLEMIDGM SAQQKSRYGK RFVDCVVQFS KETGIATNVN ANDMIPPELI SKMQKVLSDA VRRVYTEHLI SRSTAKEVAT ARGISEGTVY SYLAMAVEKG LPLHLDKLNV SRKNIAMALN AVRVHLGSNV AVLTPWVEAM GVVPDFNQLK LIRAILIYEY GLDTSENQEK PDIQSMPSTS NPSTIKTVPS TPSSSLRAPP LKKFKL //