ID KAE1B_HALWD Reviewed; 533 AA. AC Q18KI0; DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot. DT 25-JUL-2006, sequence version 1. DT 18-APR-2012, entry version 48. DE RecName: Full=Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein; DE Includes: DE RecName: Full=Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 homolog; DE AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein KAE1 homolog; DE Includes: DE RecName: Full=Probable serine/threonine-protein kinase BUD32 homolog; DE EC=2.7.11.1; GN OrderedLocusNames=HQ1341A; OS Haloquadratum walsbyi (strain DSM 16790). OC Archaea; Euryarchaeota; Halobacteria; Halobacteriales; OC Halobacteriaceae; Haloquadratum. OX NCBI_TaxID=362976; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DSM 16790; RX PubMed=16820047; DOI=10.1186/1471-2164-7-169; RA Bolhuis H., Palm P., Wende A., Falb M., Rampp M., Rodriguez-Valera F., RA Pfeiffer F., Oesterhelt D.; RT "The genome of the square archaeon Haloquadratum walsbyi: life at the RT limits of water activity."; RL BMC Genomics 7:169-169(2006). CC -!- FUNCTION: Required for the formation of a threonylcarbamoyl group CC on adenosine at position 37 (t(6)A37) in tRNAs that read codons CC beginning with adenine (By similarity). CC -!- CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. CC -!- SUBCELLULAR LOCATION: Cytoplasm (Potential). CC -!- SIMILARITY: In the N-terminal section; belongs to the KAE1 / YgjD CC family. CC -!- SIMILARITY: In the C-terminal section; belongs to the protein CC kinase superfamily. Tyr protein kinase family. BUD32 subfamily. CC -!- SIMILARITY: Contains 1 protein kinase domain. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AM180088; CAJ51469.1; -; Genomic_DNA. DR RefSeq; YP_657116.1; NC_008212.1. DR ProteinModelPortal; Q18KI0; -. DR STRING; Q18KI0; -. DR GeneID; 4194019; -. DR GenomeReviews; AM180088_GR; HQ1341A. DR KEGG; hwa:HQ1341A; -. DR eggNOG; COG0533; -. DR HOGENOM; HBG304663; -. DR KO; K00924; -. DR KO; K01409; -. DR OMA; GNCLDQF; -. DR ProtClustDB; PRK09605; -. DR BioCyc; HWAL362976:HQ1341A-MONOMER; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW. DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro. DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR GO; GO:0006508; P:proteolysis; IEA:InterPro. DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW. DR HAMAP; MF_01447; Kae1_Bud32_arch; 1; -. DR InterPro; IPR022449; Kae1_pept_M22-rel. DR InterPro; IPR022495; Kinase-assoc_endopept-1. DR InterPro; IPR011009; Kinase-like_dom. DR InterPro; IPR009220; Pept_M22_Gylco. DR InterPro; IPR000905; Peptidase_M22. DR InterPro; IPR017861; Peptidase_M22_subgr. DR InterPro; IPR000719; Prot_kinase_cat_dom. DR Pfam; PF00814; Peptidase_M22; 1. DR PIRSF; PIRSF036401; Gcp_STYKS; 1. DR PRINTS; PR00789; OSIALOPTASE. DR SUPFAM; SSF56112; Kinase_like; 1. DR TIGRFAMs; TIGR03724; Arch_bud32; 1. DR TIGRFAMs; TIGR03722; Arch_KAE1; 1. DR TIGRFAMs; TIGR00329; Gcp_kae1; 1. DR PROSITE; PS01016; GLYCOPROTEASE; FALSE_NEG. DR PROSITE; PS00107; PROTEIN_KINASE_ATP; FALSE_NEG. DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1. DR PROSITE; PS00109; PROTEIN_KINASE_TYR; FALSE_NEG. PE 3: Inferred from homology; KW ATP-binding; Complete proteome; Cytoplasm; Kinase; Nucleotide-binding; KW Serine/threonine-protein kinase; Transferase; tRNA processing. FT CHAIN 1 533 Probable bifunctional tRNA FT threonylcarbamoyladenosine biosynthesis FT protein. FT /FTId=PRO_0000303649. FT DOMAIN 339 533 Protein kinase. FT NP_BIND 347 354 ATP (By similarity). FT REGION 1 328 Kae1. FT ACT_SITE 452 452 Proton acceptor (By similarity). FT BINDING 363 363 ATP (By similarity). SQ SEQUENCE 533 AA; 57542 MW; A504147E944A27A2 CRC64; MRILGIEGTA WAASAALYNT HDETIVIESD PYQPDSGGLH PREAAEHMST ALPEVISTIL ERAVSSGNTD AIGIDAIAFS RGPGLGPCLR VVGTAARTLT QALSVPLIGV NHMIAHLEIG RHQSGFTTPV CLNASGANAH LLGYHRRQYQ VLGETMDTGV GNAIDKFTRH LGWNHPGGPK VEAAATDGSY HDLPYVVKGM DFSFSGIMSA AKDAVDNEVP VVDVCTGLQE TIFAMLTEVA ERALSLTGSN ELVLGGGVGQ NDRLREMLST MCTARGASFY APESRFLRDN AGMIAVLGAA MYEAGQTISV NDSAVDPTFR PDAVTVTWRD DETSVTRTPA TLDKTPVRGA EAIVRRINDH VVKRRVEKSY RHPKLDRRLR AERTRAEARL TSAARRLGVP TPLIFDADPE TGTLVFEYVG ETDLAADLTV SRCHAVGQHL GRIHNAGFVH GDPTTRNVRV DSAQNYLIDF GLGYHTDHVE DHAMDLHVFI QSVTGTASDP APLITAFESG YAETGISTVQ SRLRDIESRG RYH //