ID FHL2_HUMAN STANDARD; PRT; 279 AA. AC Q14192; Q13229; Q13644; Q9P294; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1997, sequence version 2. DT 19-SEP-2006, entry version 54. DE Four and a half LIM domains protein 2 (FHL-2) (Skeletal muscle LIM- DE protein 3) (SLIM 3) (LIM-domain protein DRAL). GN Name=FHL2; Synonyms=DRAL, SLIM3; OS Homo sapiens (Human). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; OC Catarrhini; Hominidae; Homo. OX NCBI_TaxID=9606; RN [1] RP NUCLEOTIDE SEQUENCE. RC TISSUE=Skeletal muscle; RX MEDLINE=97294674; PubMed=9150430; RA Genini M., Schwalbe P., Scholl F.A., Remppis A., Mattei M.-G., RA Schaefer B.W.; RT "Subtractive cloning and characterization of DRAL, a novel LIM-domain RT protein down-regulated in rhabdomyosarcoma."; RL DNA Cell Biol. 16:433-442(1997). RN [2] RP NUCLEOTIDE SEQUENCE. RC TISSUE=Heart; RX MEDLINE=98248917; PubMed=9573400; DOI=10.1016/S0378-1119(97)00644-6; RA Chan K.K., Tsui S.K.W., Lee S.M.Y., Luk S.C.W., Liew C.C., Fung K.P., RA Waye M.M.Y., Lee C.Y.; RT "Molecular cloning and characterization of FHL2, a novel LIM domain RT protein preferentially expressed in human heart."; RL Gene 210:345-350(1998). RN [3] RP NUCLEOTIDE SEQUENCE. RC TISSUE=Leukocyte; RX MEDLINE=20458893; PubMed=11001931; RA Tanahashi H., Tabira T.; RT "Alzheimer's disease-associated presenilin 2 interacts with DRAL, an RT LIM-domain protein."; RL Hum. Mol. Genet. 9:2281-2289(2000). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Placenta; RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899; RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F., RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L., RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E., RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C., RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J., RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H., RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W., RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A., RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G., RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C., RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M., RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E., RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.; RT "Generation and initial analysis of more than 15,000 full-length human RT and mouse cDNA sequences."; RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002). RN [5] RP NUCLEOTIDE SEQUENCE OF 127-279. RC TISSUE=Heart muscle; RX MEDLINE=96354835; PubMed=8753811; DOI=10.1006/bbrc.1996.1222; RA Morgan M.J., Madgwick A.J.A.; RT "Slim defines a novel family of LIM-proteins expressed in skeletal RT muscle."; RL Biochem. Biophys. Res. Commun. 225:632-638(1996). RN [6] RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INTERACTION WITH ZNF638. RX PubMed=11813260; DOI=10.1002/jcb.10041.abs; RA Ng E.K.O., Chan K.K., Wong C.H., Tsui S.K.W., Ngai S.M., Lee S.M.Y., RA Kotaka M., Lee C.Y., Waye M.M.Y., Fung K.P.; RT "Interaction of the heart-specific LIM domain protein, FHL2, with DNA- RT binding nuclear protein, hNP220."; RL J. Cell. Biochem. 84:556-566(2002). RN [7] RP INTERACTION WITH TTN. RX PubMed=12432079; DOI=10.1242/jcs.00181; RA Lange S., Auerbach D., McLoughlin P., Perriard E., Schafer B.W., RA Perriard J.C., Ehler E.; RT "Subcellular targeting of metabolic enzymes to titin in heart muscle RT may be mediated by DRAL/FHL-2."; RL J. Cell Sci. 115:4925-4936(2002). CC -!- SUBUNIT: Interacts with ZNF638 and TTN/titin. CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. CC -!- TISSUE SPECIFICITY: Expressed in skeletal muscle and heart. CC -!- SIMILARITY: Contains 4 LIM zinc-binding domains. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; L42176; AAA85333.1; -; mRNA. DR EMBL; U29332; AAC52073.1; -; mRNA. DR EMBL; AB038794; BAA92253.1; -; Genomic_DNA. DR EMBL; BC014397; AAH14397.1; -; mRNA. DR EMBL; U60117; AAC50794.1; -; mRNA. DR PIR; JC6565; JC6565. DR PDB; 1X4K; NMR; A=98-159. DR PDB; 1X4L; NMR; A=221-279. DR IntAct; Q14192; -. DR Ensembl; ENSG00000115641; Homo sapiens. DR KEGG; hsa:2274; -. DR H-InvDB; HIX0002345; -. DR HGNC; HGNC:3703; FHL2. DR MIM; 602633; gene. DR ArrayExpress; Q14192; -. DR RZPD-ProtExp; M0686; -. DR RZPD-ProtExp; RZPDo834D076; -. DR RZPD-ProtExp; RZPDo839F0868; -. DR GO; GO:0005634; C:nucleus; TAS. DR GO; GO:0050681; F:androgen receptor binding; NAS. DR GO; GO:0003713; F:transcription coactivator activity; NAS. DR GO; GO:0030521; P:androgen receptor signaling pathway; NAS. DR GO; GO:0045893; P:positive regulation of transcription, DNA-d...; NAS. DR InterPro; IPR001781; LIM_Zn_bd. DR Pfam; PF00412; LIM; 4. DR ProDom; PD000094; LIM; 5. DR SMART; SM00132; LIM; 4. DR PROSITE; PS00478; LIM_DOMAIN_1; 4. DR PROSITE; PS50023; LIM_DOMAIN_2; 4. KW 3D-structure; LIM domain; Metal-binding; Nuclear protein; Repeat; KW Zinc; Zinc-finger. FT CHAIN 1 279 Four and a half LIM domains protein 2. FT /FTId=PRO_0000075737. FT DOMAIN 40 92 LIM zinc-binding 1. FT DOMAIN 101 153 LIM zinc-binding 2. FT DOMAIN 162 212 LIM zinc-binding 3. FT DOMAIN 221 275 LIM zinc-binding 4. FT ZN_FING 7 31 GATA-like (Potential). FT CONFLICT 167 167 M -> G (in Ref. 1). FT CONFLICT 167 167 M -> K (in Ref. 3 and 4). FT STRAND 101 104 FT STRAND 110 112 FT STRAND 114 117 FT TURN 118 119 FT STRAND 120 123 FT TURN 124 127 FT STRAND 130 132 FT STRAND 138 144 FT TURN 145 146 FT STRAND 147 150 FT HELIX 151 157 FT TURN 158 158 FT STRAND 159 159 FT STRAND 221 221 FT TURN 222 225 FT STRAND 226 226 FT STRAND 231 233 FT STRAND 235 235 FT STRAND 237 238 FT STRAND 240 241 FT STRAND 243 244 FT TURN 246 248 FT STRAND 250 250 FT STRAND 252 254 FT STRAND 257 257 FT TURN 259 260 FT STRAND 261 261 FT STRAND 264 265 FT STRAND 267 271 FT HELIX 273 277 FT TURN 278 278 SQ SEQUENCE 279 AA; 32196 MW; DBDC77C9BD6C4BBA CRC64; MTERFDCHHC NESLFGKKYI LREESPYCVV CFETLFANTC EECGKPIGCD CKDLSYKDRH WHEACFHCSQ CRNSLVDKPF AAKEDQLLCT DCYSNEYSSK CQECKKTIMP GTRKMEYKGS SWHETCFICH RCQQPIGTKS FIPKDNQNFC VPCYEKQHAM QCVQCKMPIT TGGVTYREQP WHKECFVCTA CRKQLSGQRF TARDDFAYCL NCFCDLYAKK CAGCTNPISG LGGTKYISFE ERQWHNDCFN CKKCSLSLVG RGFLTERDDI LCPDCGKDI //