ID AROE_METBU Reviewed; 269 AA. AC Q12UJ9; DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot. DT 22-AUG-2006, sequence version 1. DT 25-OCT-2017, entry version 82. DE RecName: Full=Shikimate dehydrogenase (NADP(+)) {ECO:0000255|HAMAP-Rule:MF_00222}; DE Short=SDH {ECO:0000255|HAMAP-Rule:MF_00222}; DE EC=1.1.1.25 {ECO:0000255|HAMAP-Rule:MF_00222}; GN Name=aroE {ECO:0000255|HAMAP-Rule:MF_00222}; GN OrderedLocusNames=Mbur_1998; OS Methanococcoides burtonii (strain DSM 6242 / NBRC 107633 / OCM 468 / OS ACE-M). OC Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales; OC Methanosarcinaceae; Methanococcoides. OX NCBI_TaxID=259564; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DSM 6242 / NBRC 107633 / OCM 468 / ACE-M; RX PubMed=19404327; DOI=10.1038/ismej.2009.45; RA Allen M.A., Lauro F.M., Williams T.J., Burg D., Siddiqui K.S., RA De Francisci D., Chong K.W., Pilak O., Chew H.H., De Maere M.Z., RA Ting L., Katrib M., Ng C., Sowers K.R., Galperin M.Y., Anderson I.J., RA Ivanova N., Dalin E., Martinez M., Lapidus A., Hauser L., Land M., RA Thomas T., Cavicchioli R.; RT "The genome sequence of the psychrophilic archaeon, Methanococcoides RT burtonii: the role of genome evolution in cold adaptation."; RL ISME J. 3:1012-1035(2009). CC -!- FUNCTION: Involved in the biosynthesis of the chorismate, which CC leads to the biosynthesis of aromatic amino acids. Catalyzes the CC reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to CC yield shikimate (SA). {ECO:0000255|HAMAP-Rule:MF_00222}. CC -!- CATALYTIC ACTIVITY: Shikimate + NADP(+) = 3-dehydroshikimate + CC NADPH. {ECO:0000255|HAMAP-Rule:MF_00222}. CC -!- PATHWAY: Metabolic intermediate biosynthesis; chorismate CC biosynthesis; chorismate from D-erythrose 4-phosphate and CC phosphoenolpyruvate: step 4/7. {ECO:0000255|HAMAP-Rule:MF_00222}. CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00222}. CC -!- SIMILARITY: Belongs to the shikimate dehydrogenase family. CC {ECO:0000255|HAMAP-Rule:MF_00222}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; CP000300; ABE52877.1; -; Genomic_DNA. DR RefSeq; WP_011500018.1; NC_007955.1. DR ProteinModelPortal; Q12UJ9; -. DR SMR; Q12UJ9; -. DR STRING; 259564.Mbur_1998; -. DR EnsemblBacteria; ABE52877; ABE52877; Mbur_1998. DR GeneID; 3996950; -. DR KEGG; mbu:Mbur_1998; -. DR eggNOG; arCOG01033; Archaea. DR eggNOG; COG0169; LUCA. DR HOGENOM; HOG000237875; -. DR KO; K00014; -. DR OMA; PLIHNAC; -. DR OrthoDB; POG093Z09ZR; -. DR UniPathway; UPA00053; UER00087. DR Proteomes; UP000001979; Chromosome. DR GO; GO:0050661; F:NADP binding; IEA:InterPro. DR GO; GO:0004764; F:shikimate 3-dehydrogenase (NADP+) activity; IEA:UniProtKB-EC. DR GO; GO:0009073; P:aromatic amino acid family biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0009423; P:chorismate biosynthetic process; IEA:UniProtKB-UniPathway. DR GO; GO:0019632; P:shikimate metabolic process; IEA:InterPro. DR HAMAP; MF_00222; Shikimate_DH_AroE; 1. DR InterPro; IPR036291; NAD(P)-bd_dom_sf. DR InterPro; IPR011342; Shikimate_DH. DR InterPro; IPR013708; Shikimate_DH-bd_N. DR InterPro; IPR022893; Shikimate_DH_fam. DR InterPro; IPR006151; Shikm_DH/Glu-tRNA_Rdtase. DR Pfam; PF01488; Shikimate_DH; 1. DR Pfam; PF08501; Shikimate_dh_N; 1. DR SUPFAM; SSF51735; SSF51735; 1. DR TIGRFAMs; TIGR00507; aroE; 1. PE 3: Inferred from homology; KW Amino-acid biosynthesis; Aromatic amino acid biosynthesis; KW Complete proteome; NADP; Oxidoreductase; Reference proteome. FT CHAIN 1 269 Shikimate dehydrogenase (NADP(+)). FT /FTId=PRO_1000021302. FT NP_BIND 124 128 NADP. {ECO:0000255|HAMAP-Rule:MF_00222}. FT NP_BIND 147 152 NADP. {ECO:0000255|HAMAP-Rule:MF_00222}. FT REGION 15 17 Shikimate binding. {ECO:0000255|HAMAP- FT Rule:MF_00222}. FT ACT_SITE 66 66 Proton acceptor. {ECO:0000255|HAMAP- FT Rule:MF_00222}. FT BINDING 62 62 Shikimate. {ECO:0000255|HAMAP- FT Rule:MF_00222}. FT BINDING 86 86 Shikimate. {ECO:0000255|HAMAP- FT Rule:MF_00222}. FT BINDING 100 100 Shikimate. {ECO:0000255|HAMAP- FT Rule:MF_00222}. FT BINDING 211 211 NADP; via carbonyl oxygen. FT {ECO:0000255|HAMAP-Rule:MF_00222}. FT BINDING 213 213 Shikimate. {ECO:0000255|HAMAP- FT Rule:MF_00222}. FT BINDING 234 234 NADP; via carbonyl oxygen. FT {ECO:0000255|HAMAP-Rule:MF_00222}. SQ SEQUENCE 269 AA; 28790 MW; B5A787654C8B454A CRC64; MKKIFAVLGD PIEHSLSPIM HNSAFEALDM DCTYHAFRVE KNDLENALQG AKAMGFGGLN LTVPLKETAL KFVDADSLAA KIGAINTIDF KDGIKGYNTD GIGAKRTIED EGVDIKDKNV LILGAGGAAR AIAFTFAEAG ANVNIANRTP ERAMQLAAEI GDAKGYGLDI VDNGLEDIDI LINTTTVGLG NSNGTLVTAE QMHSDLAVFD IVYNPLMTKL LQEAETAGAR PITGIMMLVY QGAEAFRIWT GKEPPINVMK KTVMETLDI //