ID DAD1_YEAST STANDARD; PRT; 94 AA. AC Q12248; DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 25-JUL-2006, entry version 33. DE DASH complex subunit DAD1 (Outer kinetochore protein DAD1) (DUO1 and DE DAM1-interacting protein 1). GN Name=DAD1; OrderedLocusNames=YDR016C; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX MEDLINE=97313263; PubMed=9169867; RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., RA Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., RA Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., RA Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., RA Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., RA Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., RA Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., RA Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., RA Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., RA Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., RA Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., RA Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., RA Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., RA Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., RA Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., RA Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., RA Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., RA Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., RA Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., RA Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., RA Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., RA Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., RA Waterston R., Albermann K., Hani J., Heumann K., Kleine K., RA Mewes H.-W., Zollner A., Zaccaria P.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."; RL Nature 387:75-78(1997). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RA Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E., RA Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., RA Zuo D., Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., RA Gonzaga L., Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., RA LaBaer J.; RT "Creation of the YFLEX clone resource: cloning of Saccharomyces RT cerevisiae ORFs in the Gateway recombinational cloning system."; RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases. RN [3] RP COMPONENT OF DASH COMPLEX. RX PubMed=11799062; DOI=10.1101/gad.959402; RA Li Y., Bachant J.B., Alcasabas A.A., Wang Y., Qin J., Elledge S.J.; RT "The mitotic spindle is required for loading of the DASH complex onto RT the kinetochore."; RL Genes Dev. 16:183-197(2002). RN [4] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923954; PubMed=14562095; DOI=10.1038/nature02026; RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., RA Weissman J.S., O'Shea E.K.; RT "Global analysis of protein localization in budding yeast."; RL Nature 425:686-691(2003). RN [5] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [6] RP FUNCTION. RX PubMed=15664196; DOI=10.1016/j.molcel.2004.12.019; RA Westermann S., Avila-Sakar A., Wang H.-W., Niederstrasser H., Wong J., RA Drubin D.G., Nogales E., Barnes G.; RT "Formation of a dynamic kinetochore-microtubule interface through RT assembly of the Dam1 ring complex."; RL Mol. Cell 17:277-290(2005). RN [7] RP ELECTRON MICROSCOPY OF DASH COMPLEX ALONE AND BOUND TO MICROTUBULES. RX PubMed=15640796; DOI=10.1038/nsmb896; RA Miranda J.L., Wulf P.D., Sorger P.K., Harrison S.C.; RT "The yeast DASH complex forms closed rings on microtubules."; RL Nat. Struct. Mol. Biol. 12:138-143(2005). CC -!- FUNCTION: Component of the DASH complex, a microtubule-binding CC subcomplex of the outer kinetochore that is essential for proper CC chromosome segregation. The DASH complex mediates the formation CC and maintenance of bipolar kinetochore-microtubule attachments by CC forming closed rings around spindle microtubules and establishing CC interactions with proteins from the central kinetochore. The DASH CC ring complex may both stabilize microtubules during chromosome CC attachment in anaphase A, and allow the chromosome to remain CC attached to the depolymerizing microtubule in anaphase B. CC Microtubule depolymerization proceeds by protofilament splaying CC and may induce the kinetochore-attached ring to slide CC longitudinally, thereby helping to transduce depolymerization CC energy into pulling forces to disjoin chromatids. CC -!- SUBUNIT: The DASH complex is an approximately 210 kDa CC heterodecamer, which consists of ASK1, DAD1, DAD2, DAD3, DAD4, CC DAM1, DUO1, HSK3, SPC19 and SPC34, with an apparent stoichiometry CC of one copy of each subunit. DASH oligomerizes to form rings that CC encircle the microtubules. Integrity of the complex and CC interactions with central kinetochore proteins are regulated by CC the spindle assembly checkpoint kinase IPL1. CC -!- INTERACTION: CC P53267:DAM1; NbExp=1; IntAct=EBI-35662, EBI-23268; CC P53168:DUO1; NbExp=2; IntAct=EBI-35662, EBI-23800; CC P36131:SPC34; NbExp=1; IntAct=EBI-35662, EBI-26401; CC -!- SUBCELLULAR LOCATION: Nucleus. Associates with the mitotic spindle CC and the kinetochore. CC -!- MISCELLANEOUS: Present with 799 molecules/cell. CC -!- SIMILARITY: Belongs to the DASH complex DAD1 family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Z74312; CAA98836.1; -; Genomic_DNA. DR EMBL; Z49770; CAA89841.1; -; Genomic_DNA. DR EMBL; AY557635; AAS55961.1; -; Genomic_DNA. DR PIR; S54639; S54639. DR IntAct; Q12248; -. DR Ensembl; YDR016C; Saccharomyces cerevisiae. DR GenomeReviews; Z71256_GR; YDR016C. DR SGD; S000002423; DAD1. DR LinkHub; Q12248; -. DR GO; GO:0042729; C:DASH complex; IDA. DR GO; GO:0005819; C:spindle; IPI. DR GO; GO:0005515; F:protein binding; IPI. DR GO; GO:0005200; F:structural constituent of cytoskeleton; IPI. DR GO; GO:0030472; P:mitotic spindle organization and biogenesis...; IPI. DR GO; GO:0031110; P:regulation of microtubule polymerization or...; IDA. KW Cell cycle; Cell division; Centromere; Chromosome partition; KW Complete proteome; Microtubule; Mitosis; Nuclear protein. FT CHAIN 1 94 DASH complex subunit DAD1. FT /FTId=PRO_0000127613. SQ SEQUENCE 94 AA; 10516 MW; 6D27737AC5A39D51 CRC64; MMASTSNDEE KLISTTDKYF IEQRNIVLQE INETMNSILN GLNGLNISLE SSIAVGREFQ SVSDLWKTLY DGLESLSDEA PIDEQPTLSQ SKTK //