ID PNU1_SCHPO STANDARD; PRT; 322 AA. AC Q10480; Q9HFA0; DT 01-OCT-1996 (Rel. 34, Created) DT 10-OCT-2003 (Rel. 42, Last sequence update) DT 15-MAR-2004 (Rel. 43, Last annotation update) DE Nuclease 1, mitochondrial precursor (EC 3.1.30.-) (SpNUC1). GN PNU1 OR NUC1 OR SPAC17C9.08. OS Schizosaccharomyces pombe (Fission yeast). OC Eukaryota; Fungi; Ascomycota; Schizosaccharomycetes; OC Schizosaccharomycetales; Schizosaccharomycetaceae; OC Schizosaccharomyces. OX NCBI_TaxID=4896; RN [1] RP SEQUENCE FROM N.A., FUNCTION, COFACTOR, AND SUBCELLULAR LOCATION. RC STRAIN=ATCC 87289; RX MEDLINE=21299058; PubMed=11406279; RA Ikeda S., Kawasaki N.; RT "Isolation and characterization of the Schizosaccharomyces pombe cDNA RT encoding the mitochondrial endonuclease."; RL Biochim. Biophys. Acta 1519:111-116(2001). RN [2] RP SEQUENCE FROM N.A. RC STRAIN=972; RX MEDLINE=21848401; PubMed=11859360; RA Wood V., Gwilliam R., Rajandream M.A., Lyne M., Lyne R., Stewart A., RA Sgouros J., Peat N., Hayles J., Baker S., Basham D., Bowman S., RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., RA Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., RA Gentles S., Goble A., Hamlin N., Harris D., Hidalgo J., Hodgson G., RA Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., RA James K., Jones L., Jones M., Leather S., McDonald S., McLean J., RA Mooney P., Moule S., Mungall K., Murphy L., Niblett D., Odell C., RA Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., RA Rutherford K., Rutter S., Saunders D., Seeger K., Sharp S., RA Skelton J., Simmonds M., Squares R., Squares S., Stevens K., RA Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., RA Woodward J., Volckaert G., Aert R., Robben J., Grymonprez B., RA Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., RA Gabel C., Fuchs M., Fritzc C., Holzer E., Moestl D., Hilbert H., RA Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., RA Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., RA Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., RA Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., RA Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., RA Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., RA Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., RA Cerrutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., RA Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.; RT "The genome sequence of Schizosaccharomyces pombe."; RL Nature 415:871-880(2002). CC -!- FUNCTION: This enzyme has both RNase and DNase activity. It CC degrades single-stranded DNA and RNA. CC -!- COFACTOR: Requires manganese or magnesium. CC -!- SUBUNIT: Homodimer (By similarity). CC -!- SUBCELLULAR LOCATION: Mitochondrial inner membrane. CC -!- SIMILARITY: Belongs to the DNA/RNA non-specific endonuclease CC family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AB050780; BAB20882.1; -. DR EMBL; Z73099; CAA97354.1; -. DR PIR; T11588; T11588. DR GeneDB_SPombe; SPAC17C9.08; -. DR GO; GO:0005739; C:mitochondrion; ISS. DR GO; GO:0006139; P:nucleobase, nucleoside, nucleotide and nucl...; ISS. DR GO; GO:0006950; P:response to stress; ISS. DR InterPro; IPR001604; Endonuclease. DR Pfam; PF01223; Endonuclease; 1. DR SMART; SM00477; NUC; 1. DR PROSITE; PS01070; NUCLEASE_NON_SPEC; 1. KW Hydrolase; Nuclease; Endonuclease; Magnesium; Manganese; KW Mitochondrion; Transit peptide; Inner membrane. FT TRANSIT 1 ? MITOCHONDRION. FT CHAIN ? 322 NUCLEASE 1. FT ACT_SITE 142 142 BY SIMILARITY. FT CONFLICT 321 322 GK -> VFLVFPVIVLYYENI (IN REF. 2). SQ SEQUENCE 322 AA; 36428 MW; C3AB9C38801563A5 CRC64; MSSNLIKSFG LIAIGAISGV TFTHFYYKGY QGSDVPDLTP RYTKFDSAGR ALESIYDFNA TKFFQYGIPG PVADQRVNHG YMSVFDRRTR NPFYTAETIT QESLNQRKGN RRYSEFVPDD NIPEMFQAKL GDYRGSGYDR GHQVPAADCK FSQEAMNETF YLSNMCPQVG DGFNRNYWAY FEDWCRRLTS KYGSVTIMTG PLYLPKKNER GQWEVQYRVI GNPPNVAVPT HFFKVIIAEK SGEPTSSPSV AAFVLPNKPI ADNFPLKNFA VPVEVVERAS GLEILSNVPK GNRKQLCSEV VCQLNVKEFV ESVKQKQKNQ GK //