ID AI2_USTMA Reviewed; 533 AA. AC Q0H8X8; DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot. DT 09-JAN-2007, sequence version 2. DT 25-MAY-2022, entry version 85. DE RecName: Full=Probable intron-encoded endonuclease aI2; DE Contains: DE RecName: Full=Truncated non-functional cytochrome oxidase 1; DE Contains: DE RecName: Full=Intron-encoded endonuclease aI2; DE EC=3.1.-.-; DE Flags: Precursor; GN Name=aI2; OS Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus). OG Mitochondrion. OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago. OX NCBI_TaxID=237631; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=521 / FGSC 9021; RA Kennell J.C., Boehmer C.; RT "Annotation of mitochondrial genome of Ustilago maydis and comparative RT analysis of basidiomycete mtDNAs."; RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=521 / FGSC 9021; RX PubMed=17080091; DOI=10.1038/nature05248; RA Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., RA Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., RA Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., RA Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W., RA Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., RA Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., RA Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., RA Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., RA Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., RA Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., RA Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., RA Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., RA Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., RA Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., RA Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., RA Birren B.W.; RT "Insights from the genome of the biotrophic fungal plant pathogen Ustilago RT maydis."; RL Nature 444:97-101(2006). CC -!- FUNCTION: Mitochondrial DNA endonuclease involved in intron homing. CC {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Mitochondrion. Membrane {ECO:0000305}; Multi-pass CC membrane protein {ECO:0000305}. CC -!- PTM: The mature protein may arise from proteolytic cleavage of an in- CC frame translation of COX1 exons 1 and 2 plus intron 2, containing the CC aI2 open reading frame. CC -!- SIMILARITY: In the C-terminal section; belongs to the LAGLIDADG CC endonuclease family. {ECO:0000305}. CC -!- SIMILARITY: In the N-terminal section; belongs to the heme-copper CC respiratory oxidase family. {ECO:0000305}. CC -!- SEQUENCE CAUTION: CC Sequence=AAZ67028.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; DQ157700; AAZ67028.1; ALT_SEQ; Genomic_DNA. DR EMBL; AACP01000277; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR RefSeq; YP_762694.1; NC_008368.1. DR AlphaFoldDB; Q0H8X8; -. DR SMR; Q0H8X8; -. DR PRIDE; Q0H8X8; -. DR GeneID; 4308276; -. DR InParanoid; Q0H8X8; -. DR Proteomes; UP000000561; Mitochondrion. DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW. DR GO; GO:0005751; C:mitochondrial respiratory chain complex IV; IBA:GO_Central. DR GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:InterPro. DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW. DR GO; GO:0020037; F:heme binding; IEA:InterPro. DR GO; GO:0009060; P:aerobic respiration; IBA:GO_Central. DR GO; GO:0015990; P:electron transport coupled proton transport; IBA:GO_Central. DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW. DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; IBA:GO_Central. DR GO; GO:0022904; P:respiratory electron transport chain; IBA:GO_Central. DR Gene3D; 1.20.210.10; -; 1. DR Gene3D; 3.10.28.10; -; 2. DR InterPro; IPR023616; Cyt_c_oxase-like_su1_dom. DR InterPro; IPR036927; Cyt_c_oxase-like_su1_sf. DR InterPro; IPR000883; Cyt_C_Oxase_1. DR InterPro; IPR027434; Homing_endonucl. DR InterPro; IPR004860; LAGLIDADG_2. DR PANTHER; PTHR10422; PTHR10422; 1. DR Pfam; PF00115; COX1; 1. DR Pfam; PF00961; LAGLIDADG_1; 2. DR PRINTS; PR01165; CYCOXIDASEI. DR SUPFAM; SSF55608; SSF55608; 2. DR SUPFAM; SSF81442; SSF81442; 1. DR PROSITE; PS50855; COX1; 1. PE 3: Inferred from homology; KW Endonuclease; Hydrolase; Intron homing; Membrane; Mitochondrion; Nuclease; KW Reference proteome; Transmembrane; Transmembrane helix. FT CHAIN 1..? FT /note="Truncated non-functional cytochrome oxidase 1" FT /id="PRO_0000271153" FT CHAIN ?..533 FT /note="Intron-encoded endonuclease aI2" FT /id="PRO_0000271154" FT TRANSMEM 15..35 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 56..76 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 99..119 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 135..155 FT /note="Helical" FT /evidence="ECO:0000255" FT REGION 1..128 FT /note="COX1 exons 1 and 2 encoded" FT REGION 129..533 FT /note="COX1 intron 2 encoded" SQ SEQUENCE 533 AA; 60941 MW; 589865A7700935A0 CRC64; MVRWLYSTNA KDIGTLYLIF AVFAAMIGTA FSVLIRMELA APGVQYLNGD HQLYNVIITA HAFVMIFFMV MPAMVGGFGN YLVPVMIGAP DMAFPRLNNI SFWLLPPSLI LLLASAFVEQ GAGTGWTVKC KLSQIIIMFV SIYNLLNITR CGKLLYSEMN THSVLTYIST MFNDVKMSST WGQSAWILYK IIIPRGITYI KLVVGVLSYT MYKVYTYSIS LFYVVYNSVS FNKNPSETQR NVFSSGRGVN NSTNFKEWLV GITDGDGTFY FAKTKKGIWS FSFQIGQSNY NIRLLYYIKS MLGIGSVSVT HAKDNTAHYR VRNIQHIIQY ILPIFDTYPL LTSKYFNYDL FKKAILIMND SSLSNQEKDE KISYLKSQSL PDNYISPAWN NVNNQVTSIL DAMSVMTKSW LIGFTEAEGS FYIVKKGPLR LVHAFEISQK LDHIVLEAIA LILDIKVTTK KTYMTVVTTN YKSIENIISY FFKTMKGMKA LEYRIWARSF NKKGSGRFEY MTKIQNLMRN IRSIRLDKNF TKK //