ID GNT5_RAT STANDARD; PRT; 740 AA. AC Q08834; DT 01-FEB-1995 (REL. 31, CREATED) DT 01-FEB-1995 (REL. 31, LAST SEQUENCE UPDATE) DT 01-FEB-1995 (REL. 31, LAST ANNOTATION UPDATE) DE ALPHA-1,3(6)-MANNOSYLGLYCOPROTEIN BETA-1,6-N-ACETYL- DE GLUCOSAMINYLTRANSFERASE V (EC 2.4.1.155) (ALPHA-MANNOSIDE BETA-1,6-N- DE ACETYLGLUCOSAMINYLTRANSFERASE) (N-ACETYLGLUCOSAMINYL-TRANSFERASE V) DE (GLCNAC-T V). OS RATTUS NORVEGICUS (RAT). OC EUKARYOTA; METAZOA; CHORDATA; VERTEBRATA; TETRAPODA; MAMMALIA; OC EUTHERIA; RODENTIA. RN [1] RP SEQUENCE FROM N.A., AND SEQUENCE OF 375-386; 546-557 AND 592-607. RC TISSUE=KIDNEY; RX MEDLINE; 93340130. RA SHOREIBAH M., PERNG G.-S., ADLER B., WEINSTEIN J., BASU R., RA CUPPLES R., WEN D., BROWNE J.K., BUCKHAULTS P., FREGIEN N., RA PIERCE M.; RL J. BIOL. CHEM. 268:15381-15385(1993). CC -!- FUNCTION: CATALYZES THE ADDITION OF N-ACETYLGLUCOSAMINE IN BETA CC 1-6 LINKAGE TO THE ALPHA-LINKED MANNOSE OF BIANTENNARY N-LINKED CC OLIGOSACCHARIDES. IT IS ONE OF THE MOST IMPORTANT ENZYMES CC INVOLVED IN THE REGULATION OF THE BIOSYNTHESIS OF GLYCOPROTEIN CC OLIGOSACCHARIDES. CC -!- CATALYTIC ACTIVITY: UDP-N-ACETYL-D-GLUCOSAMINE + N-ACETYL-BETA-D- CC GLUCOSAMINYL-1,2-ALPHA-D-MANNOSYL-1,3(6)-(N-ACETYL-BETA-D- CC GLUCOSAMINYL-1,2-ALPHA-D-MANNOSYL,1,6(3))-BETA-D-MANNOSYL-1,4-N- CC ACETYL-BETA-D-GLUCOSAMINYL-R = UDP + N-ACETYL-BETA-D-GLUCOSAMINYL- CC 1,2-(N-ACETYL-BETA-D-GLUCOSAMINYL-1,6)-1,2-ALPHA-D-MANNOSYL-1,3(6) CC -(N-ACETYL-BETA-D-GLUCOSAMINYL-1,2-ALPHA-D-MANNOSYL-1,6(3))-BETA- CC D-MANNOSYL-1,4-N-ACETYL-BETA-D-GLUCOSAMINYL-R. CC -!- PATHWAY: GLYCOSYLATION. CC -!- SUBCELLULAR LOCATION: TYPE II MEMBRANE PROTEIN. GOLGI. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; L14284; L14284. DR PIR; A47134; A47134. KW TRANSFERASE; GLYCOSYLTRANSFERASE; GLYCOPROTEIN; TRANSMEMBRANE; KW SIGNAL-ANCHOR; GOLGI STACK. FT DOMAIN 1 13 CYTOPLASMIC (POTENTIAL). FT TRANSMEM 14 30 SIGNAL-ANCHOR (TYPE-II MEMBRANE PROTEIN) FT (POTENTIAL). FT DOMAIN 31 740 LUMENAL, CATALYTIC (POTENTIAL). FT CARBOHYD 109 109 POTENTIAL. FT CARBOHYD 114 114 POTENTIAL. FT CARBOHYD 117 117 POTENTIAL. FT CARBOHYD 333 333 POTENTIAL. FT CARBOHYD 432 432 POTENTIAL. FT CARBOHYD 446 446 POTENTIAL. SQ SEQUENCE 740 AA; 84561 MW; 2874884 CN; MAFFSPWKLS SQKLGFFLVT FGFIWGMMLL HFTIQQRTQP ESSSMLREQI LDLSKRYIKA LAEENRNVVD GPYAGVMTAY DLKKTLAVLL DNILQRIGKL ESKVDNLVNG TGANSTNSTT AVPSLVSLEK INVADIINGV QEKCVLPPMD GYPHCEGKIK WMKDMWRSDP CYADYGVDGT SCSFFIYLSE VENWCPRLPW RAKNPYEEAD HNSLAEIRTD FNILYGMMKK HEEFRWMRLR IRRMADAWIQ AIKSLAEKQN LEKRKRKKIL VHLGLLTKES GFKIAETAFS GGPLGELVQW SDLITSLYLL GHDIRISASL AELKEIMKKV VGNRSGCPTV GDRIVELIYI DIVGLAQFKK TLGPSWVHYQ CMLRVLDSFG TEPEFNHASY AQSKGHKTPW GKWNLNPQQF YTMFPHTPDN SFLGFVVEQH LNSSDIHHIN EIKRQNQSLV YGKVDSFWKN KKIYLDIIHT YMEVHATVYG SSTKNIPSYV KNHGILSGRD LQFLLRETKL FVGLGFPYEG PAPLEAIANG CAFLNPKFNP PKSSKNTDFF IGKPTLRELT SQHPYAEVFI GRPHVWTVDL NNREEVEDAV KAILNQKIEP YMPYEFTCEG MLQRINAFIE KQDFCHGQVM WPPLSALQVK LAEPGQSCKQ VCQESQLICE PSFFQHLNKE KDLLKYKVIC QSSELYKDIL VPSFYPKSKH CVFQGDLLLF SCAGAHPTHQ RICPCRDFIK GQVALCKDCL //