ID PIG1_YEAST Reviewed; 648 AA. AC Q06216; D6VYS0; E9P964; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 29-MAY-2024, entry version 147. DE RecName: Full=Serine/threonine-protein phosphatase 1 regulatory subunit PIG1; GN Name=PIG1; OrderedLocusNames=YLR273C; ORFNames=L9328.2; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=9169871; RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J., RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., RA Zollner A., Hani J., Hoheisel J.D.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."; RL Nature 387:87-90(1997). RN [2] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RX PubMed=24374639; DOI=10.1534/g3.113.008995; RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.; RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now."; RL G3 (Bethesda) 4:389-398(2014). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=17322287; DOI=10.1101/gr.6037607; RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., RA LaBaer J.; RT "Approaching a complete repository of sequence-verified protein-encoding RT clones for Saccharomyces cerevisiae."; RL Genome Res. 17:536-543(2007). RN [4] RP PARTIAL NUCLEOTIDE SEQUENCE, AND CHARACTERIZATION. RX PubMed=9046081; RX DOI=10.1002/(sici)1097-0061(199701)13:1<1::aid-yea49>3.0.co;2-f; RA Cheng C., Huang D., Roach P.J.; RT "Yeast PIG genes: PIG1 encodes a putative type 1 phosphatase subunit that RT interacts with the yeast glycogen synthase Gsy2p."; RL Yeast 13:1-8(1997). CC -!- FUNCTION: Regulates the activity of glycogen synthase. It is most CC probably a regulatory subunit for protein phosphatase type 1. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U17245; AAB67365.1; -; Genomic_DNA. DR EMBL; AY723846; AAU09763.1; -; Genomic_DNA. DR EMBL; BK006945; DAA09586.1; -; Genomic_DNA. DR PIR; S51409; S51409. DR RefSeq; NP_013375.1; NM_001182160.1. DR AlphaFoldDB; Q06216; -. DR SMR; Q06216; -. DR BioGRID; 31541; 51. DR DIP; DIP-2918N; -. DR IntAct; Q06216; 9. DR MINT; Q06216; -. DR STRING; 4932.YLR273C; -. DR CAZy; CBM21; Carbohydrate-Binding Module Family 21. DR iPTMnet; Q06216; -. DR PaxDb; 4932-YLR273C; -. DR PeptideAtlas; Q06216; -. DR EnsemblFungi; YLR273C_mRNA; YLR273C; YLR273C. DR GeneID; 850979; -. DR KEGG; sce:YLR273C; -. DR AGR; SGD:S000004263; -. DR SGD; S000004263; PIG1. DR VEuPathDB; FungiDB:YLR273C; -. DR eggNOG; KOG3986; Eukaryota. DR GeneTree; ENSGT00940000176558; -. DR HOGENOM; CLU_028211_0_0_1; -. DR InParanoid; Q06216; -. DR OMA; IDCEIEP; -. DR OrthoDB; 2039299at2759; -. DR BioCyc; YEAST:G3O-32372-MONOMER; -. DR Reactome; R-SCE-3322077; Glycogen synthesis. DR BioGRID-ORCS; 850979; 2 hits in 10 CRISPR screens. DR PRO; PR:Q06216; -. DR Proteomes; UP000002311; Chromosome XII. DR RNAct; Q06216; Protein. DR GO; GO:0000164; C:protein phosphatase type 1 complex; ISA:SGD. DR GO; GO:2001069; F:glycogen binding; IBA:GO_Central. DR GO; GO:0008157; F:protein phosphatase 1 binding; IBA:GO_Central. DR GO; GO:0019888; F:protein phosphatase regulator activity; IMP:SGD. DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-KW. DR GO; GO:0005979; P:regulation of glycogen biosynthetic process; IGI:SGD. DR Gene3D; 2.60.40.2440; Carbohydrate binding type-21 domain; 1. DR InterPro; IPR005036; CBM21_dom. DR InterPro; IPR038175; CBM21_dom_sf. DR PANTHER; PTHR12307; PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT; 1. DR PANTHER; PTHR12307:SF51; SERINE_THREONINE-PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT GAC1-RELATED; 1. DR Pfam; PF03370; CBM_21; 1. DR PROSITE; PS51159; CBM21; 1. PE 1: Evidence at protein level; KW Glycogen biosynthesis; Reference proteome. FT CHAIN 1..648 FT /note="Serine/threonine-protein phosphatase 1 regulatory FT subunit PIG1" FT /id="PRO_0000071519" FT DOMAIN 201..331 FT /note="CBM21" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00491" FT REGION 20..52 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 593..629 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 593..615 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT CONFLICT 228 FT /note="I -> K (in Ref. 3; AAU09763)" FT /evidence="ECO:0000305" SQ SEQUENCE 648 AA; 74146 MW; B95853040B225BD7 CRC64; MPYSHGKKLK PSLKLAKTIS TSSFVSSTTS NSFSPLEDST SASSSTSSSS SGKSVRFAAH LYTVKKFNTK LAPISISEKA ASNLTRNLHN NAIPLTFPFI GGEDHRYSLD ILDYSDLEYD NKDVEYDNES DVEDNAMLMH DRSMFIEKEI LCFGEEETFD MADWKLVSNN LNPFKSDYKV DVTGLEDKIF KYLNGQNIKV HSLELSDPVS YEDICSNNFG NCQIWGLIFV NNLNFEKKIE IKFTLNNWAD IHYINAHYNK SVTPHVDEFK FIIDISALKL NLISKNLIYT NFFERKTTCL LNLQFCCRYD VNGFEYRSFY DNNDYKNYEI TISLSAINLN RAVSNSSIFN SNLGPSKMGA SNAEVTMSKN NENSKKPLRK FIKDTDYYND SPLKHKFYQS FETKAACKTE PVSQTFKAET IDCEIEPFNY FFEPPDSQTN EDMSDSSYDL SLQDFNYWEF SNHGLGKALA DSDILQFKNY PKPEPFSRPP IIDDTFTLNT DDRTLGSKTQ KLEDNLAKEW KSAKTRTTLN ETPLHDDEHR TSFTYTTWNN STDTLMKRKE ERPVESASCS QLSIATIKAE EDLLYQDYIN SGRESSSPEI SPLNTTTSLP FFPGDNMSDS SGEYEERTSL SPNKIHIFRD YFYKSPSP //