ID YL301_YEAST Reviewed; 244 AA. AC Q05905; D6VYU5; DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 05-APR-2011, entry version 71. DE RecName: Full=Uncharacterized protein YLR301W; GN OrderedLocusNames=YLR301W; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; OC Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204511 / S288c / AB972; RX MEDLINE=97313267; PubMed=9169871; RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., RA Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., RA Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., RA Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., RA Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., RA Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., RA Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., RA Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., RA Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., RA Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., RA Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., RA Hani J., Hoheisel J.D.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."; RL Nature 387:87-90(1997). RN [2] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RG Saccharomyces Genome Database; RL Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=17322287; DOI=10.1101/gr.6037607; RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., RA Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., RA Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., RA Kolodner R.D., LaBaer J.; RT "Approaching a complete repository of sequence-verified protein- RT encoding clones for Saccharomyces cerevisiae."; RL Genome Res. 17:536-543(2007). RN [4] RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923954; PubMed=14562095; DOI=10.1038/nature02026; RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., RA Weissman J.S., O'Shea E.K.; RT "Global analysis of protein localization in budding yeast."; RL Nature 425:686-691(2003). RN [5] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). RN [6] RP INTERACTION WITH SEC72. RX PubMed=12518317; DOI=10.1002/yea.954; RA Willer M., Jermy A.J., Young B.P., Stirling C.J.; RT "Identification of novel protein-protein interactions at the cytosolic RT surface of the Sec63 complex in the yeast ER membrane."; RL Yeast 20:133-148(2003). RN [7] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, AND MASS RP SPECTROMETRY. RC STRAIN=ADR376; RX PubMed=17330950; DOI=10.1021/pr060559j; RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., RA Elias J.E., Gygi S.P.; RT "Large-scale phosphorylation analysis of alpha-factor-arrested RT Saccharomyces cerevisiae."; RL J. Proteome Res. 6:1190-1197(2007). RN [8] RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, AND MASS RP SPECTROMETRY. RX PubMed=18407956; DOI=10.1074/mcp.M700468-MCP200; RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.; RT "A multidimensional chromatography technology for in-depth RT phosphoproteome analysis."; RL Mol. Cell. Proteomics 7:1389-1396(2008). CC -!- FUNCTION: Unknown. Non essential. CC -!- SUBUNIT: May interact with SEC72. CC -!- INTERACTION: CC P10592:SSA2; NbExp=1; IntAct=EBI-37584, EBI-8603; CC -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. CC -!- MISCELLANEOUS: Present with 15400 molecules/cell in log phase SD CC medium. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U17243; AAB67346.1; -; Genomic_DNA. DR EMBL; AY558217; AAS56543.1; -; Genomic_DNA. DR EMBL; BK006945; DAA09611.1; -; Genomic_DNA. DR PIR; S50385; S50385. DR RefSeq; NP_013404.1; NM_001182189.1. DR ProteinModelPortal; Q05905; -. DR DIP; DIP-4329N; -. DR IntAct; Q05905; 5. DR MINT; MINT-534652; -. DR STRING; Q05905; -. DR PeptideAtlas; Q05905; -. DR PRIDE; Q05905; -. DR EnsemblFungi; YLR301W; YLR301W; YLR301W. DR GeneID; 851008; -. DR NMPDR; fig|4932.3.peg.4422; -. DR CYGD; YLR301w; -. DR SGD; S000004292; YLR301W. DR eggNOG; fuNOG09994; -. DR HOGENOM; HBG202530; -. DR OMA; FRELWQP; -. DR OrthoDB; EOG4RR9SV; -. DR PhylomeDB; Q05905; -. DR NextBio; 967561; -. DR ArrayExpress; Q05905; -. DR Genevestigator; Q05905; -. DR GermOnline; YLR301W; Saccharomyces cerevisiae. DR GO; GO:0005789; C:endoplasmic reticulum membrane; IPI:SGD. DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell. DR GO; GO:0005515; F:protein binding; IPI:IntAct. DR GO; GO:0006613; P:cotranslational protein targeting to membrane; IPI:SGD. PE 1: Evidence at protein level; KW Complete proteome; Cytoplasm; Nucleus; Phosphoprotein. FT CHAIN 1 244 Uncharacterized protein YLR301W. FT /FTId=PRO_0000245329. FT MOD_RES 143 143 Phosphoserine. SQ SEQUENCE 244 AA; 27501 MW; 80D813586A1930BB CRC64; MPALLKRLLF QVGPHPNERT FTLSSVSTDG HYISLRPFVK PSGDELSFPF EWAFAGTNET VKANDQGNGV VTQDFNFWLD TNVYLNVPNT HRGEVNTTWK NWDSGCVEET GAVYPFGADK ESVSFRELWQ PVDPSREDLV IVSPNNEKFS SNARSIVLKV TDEAYDGLVI VIGRWIQGFL SQKNNNTIEG LNFIRLLEKD SGKSEFLLSY GKEVNKIPQS YENLKKGSTV TSNGLNWEVI EYHA //