ID IF31_YEAST STANDARD; PRT; 265 AA. AC Q05775; DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 17-OCT-2006, entry version 44. DE Probable eukaryotic translation initiation factor 3 30 kDa subunit DE (eIF-3 30 kDa) (eIF3j). GN Name=HCR1; OrderedLocusNames=YLR192C; ORFNames=L8167.11; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204511 / S288c / AB972; RX MEDLINE=97313267; PubMed=9169871; RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., RA Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., RA Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., RA Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., RA Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., RA Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., RA Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., RA Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., RA Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., RA Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., RA Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., RA Hani J., Hoheisel J.D.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."; RL Nature 387:87-90(1997). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RA Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E., RA Hu Y., Vannberg F., Weger J., Kramer J., Moreira D., Kelley F., RA Zuo D., Raphael J., Hogle C., Jepson D., Williamson J., Camargo A., RA Gonzaga L., Vasconcelos A.T., Simpson A., Kolodner R., Harlow E., RA LaBaer J.; RT "Creation of the YFLEX clone resource: cloning of Saccharomyces RT cerevisiae ORFs in the Gateway recombinational cloning system."; RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases. RN [3] RP CHARACTERIZATION. RX MEDLINE=99419035; PubMed=10488093; DOI=10.1074/jbc.274.39.27567; RA Valasek L., Hasek J., Trachsel H., Imre E.M., Ruis H.; RT "The Saccharomyces cerevisiae HCR1 gene encoding a homologue of the RT p35 subunit of human translation initiation factor 3 (eIF3) is a high RT copy suppressor of a temperature-sensitive mutation in the Rpg1p RT subunit of yeast eIF3."; RL J. Biol. Chem. 274:27567-27572(1999). RN [4] RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS]. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). CC -!- FUNCTION: Probable component of the eukaryotic translation CC initiation factor 3 (eIF-3) complex, which is involved in protein CC synthesis and, together with other initiation factors, stimulates CC binding of mRNA and methionyl-tRNAi to the 40S ribosome. CC -!- SUBUNIT: Probable component of the eukaryotic translation CC initiation factor 3 (eIF-3) complex. Is not part of the eIF-3 core CC complex, with which it is associated in substochiometric amounts. CC -!- INTERACTION: CC P22696:ESS1; NbExp=1; IntAct=EBI-8944, EBI-6679; CC P33203:PRP40; NbExp=1; IntAct=EBI-8944, EBI-701; CC Q03195:RLI1; NbExp=1; IntAct=EBI-8944, EBI-35146; CC P39940:RSP5; NbExp=1; IntAct=EBI-8944, EBI-16219; CC P46995:SET2; NbExp=1; IntAct=EBI-8944, EBI-16985; CC P43582:YFL010C; NbExp=1; IntAct=EBI-8944, EBI-22766; CC Q06525:YPR152C; NbExp=1; IntAct=EBI-8944, EBI-35138; CC -!- MISCELLANEOUS: Present with 17900 molecules/cell. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U14913; AAB67433.1; -; Genomic_DNA. DR EMBL; AY557943; AAS56269.1; -; Genomic_DNA. DR PIR; S48545; S48545. DR IntAct; Q05775; -. DR GermOnline; 142254; -. DR Ensembl; YLR192C; Saccharomyces cerevisiae. DR GenomeReviews; Y13138_GR; YLR192C. DR SGD; S000004182; HCR1. DR BioCyc; SCER-S28-01:SCER-S28-01-004076-MONOMER; -. DR LinkHub; Q05775; -. DR GO; GO:0005852; C:eukaryotic translation initiation factor 3 ...; IPI. DR GO; GO:0005515; F:protein binding; IPI. DR GO; GO:0019843; F:rRNA binding; IDA. DR GO; GO:0003743; F:translation initiation factor activity; IPI. DR GO; GO:0030490; P:processing of 20S pre-rRNA; IMP. DR GO; GO:0006412; P:protein biosynthesis; IPI. DR GO; GO:0000723; P:telomere maintenance; IMP. DR InterPro; IPR013906; eIF3_subunit. KW Complete proteome; Initiation factor; Protein biosynthesis. FT CHAIN 1 265 Probable eukaryotic translation FT initiation factor 3 30 kDa subunit. FT /FTId=PRO_0000123509. SQ SEQUENCE 265 AA; 29564 MW; BDDBC5943DD7F9BB CRC64; MSWDDEAING SMGNDDAVLM DSWDAEIGDD EPVMQSWDAE EEEKKPAPKP KKEQPKKVKK GKESSADRAL LDIDTLDEKT RKELIKKAEM ESDLNNAADL FAGLGVAEEH PRARALQKEQ EEQALKRPAF TKDTPIETHP LFNAETKREY QDLRKALTAA ITPMNKKSPL NYSSSLAIDL IRDVAKPMSI ESIRQTVATL NVLIKDKERE ERQARLARVR GGTATGGAGK KKVKGKTNLG GAFKKDQDFD LDGPDDFEFG DDDFM //