ID GLR1_DROME Reviewed; 991 AA. AC Q03445; B6IDJ5; Q86PC2; Q9VRX9; DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot. DT 21-JUN-2005, sequence version 2. DT 12-OCT-2022, entry version 174. DE RecName: Full=Glutamate receptor 1; DE AltName: Full=Glutamate receptor I; DE Short=dGLUR-I; DE AltName: Full=Kainate-selective glutamate receptor; DE Flags: Precursor; GN Name=GluRIA; Synonyms=Glu-RI; ORFNames=CG8442; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; OC Drosophilidae; Drosophila; Sophophora. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE. RC STRAIN=Canton-S; TISSUE=Embryo; RX PubMed=1359540; DOI=10.1073/pnas.89.21.10484; RA Ultsch A., Schuster C.M., Laube B., Schloss P., Schmitt B., Betz H.; RT "Glutamate receptors of Drosophila melanogaster: cloning of a kainate- RT selective subunit expressed in the central nervous system."; RL Proc. Natl. Acad. Sci. U.S.A. 89:10484-10488(1992). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., RA Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [3] RP GENOME REANNOTATION. RC STRAIN=Berkeley; RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic RT review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). RC STRAIN=Berkeley; TISSUE=Embryo; RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Booth B., RA Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., RA George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., RA Miranda A., Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., RA Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., RA Celniker S.E.; RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Receptor for glutamate. L-glutamate acts as an excitatory CC neurotransmitter at many synapses in the central nervous system. The CC postsynaptic actions of Glu are mediated by a variety of receptors that CC are named according to their selective agonists (By similarity). Forms CC ligand-gated ion channels which are activated by kainate. {ECO:0000250, CC ECO:0000269|PubMed:1359540}. CC -!- SUBUNIT: Homooligomer. {ECO:0000250}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:1359540}; CC Multi-pass membrane protein {ECO:0000269|PubMed:1359540}. Postsynaptic CC cell membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=2; CC Name=1; Synonyms=A; CC IsoId=Q03445-1; Sequence=Displayed; CC Name=2; CC IsoId=Q03445-2; Sequence=VSP_014219; CC -!- TISSUE SPECIFICITY: Central nervous system. CC {ECO:0000269|PubMed:1359540}. CC -!- DEVELOPMENTAL STAGE: No expression is seen in early embryogenesis, CC whereas high expression occurs in late embryos. During larval CC development, expression decreases to undetectable levels in late CC larvae, resumes at the early pupal stage and gradually increases in CC late pupae and early adult flies. High levels of expression coincide CC with major stages of neurogenesis. {ECO:0000269|PubMed:1359540}. CC -!- SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) CC family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M97192; AAA28575.1; -; mRNA. DR EMBL; AE014296; AAF50652.2; -; Genomic_DNA. DR EMBL; BT003218; AAO24973.1; -; mRNA. DR EMBL; BT050435; ACJ13142.1; -; mRNA. DR PIR; A46421; A46421. DR RefSeq; NP_476855.1; NM_057507.4. [Q03445-1] DR PDB; 5DT6; X-ray; 1.60 A; A=474-594, A=739-880. DR PDB; 5ICT; X-ray; 1.68 A; A=474-594, A=739-880. DR PDBsum; 5DT6; -. DR PDBsum; 5ICT; -. DR AlphaFoldDB; Q03445; -. DR SMR; Q03445; -. DR BioGRID; 64192; 1. DR IntAct; Q03445; 1. DR STRING; 7227.FBpp0076691; -. DR GlyGen; Q03445; 9 sites. DR PaxDb; Q03445; -. DR PRIDE; Q03445; -. DR EnsemblMetazoa; FBtr0076982; FBpp0076691; FBgn0004619. [Q03445-1] DR GeneID; 38742; -. DR KEGG; dme:Dmel_CG8442; -. DR CTD; 38742; -. DR FlyBase; FBgn0004619; GluRIA. DR VEuPathDB; VectorBase:FBgn0004619; -. DR eggNOG; KOG1054; Eukaryota. DR GeneTree; ENSGT00940000168258; -. DR HOGENOM; CLU_007257_1_2_1; -. DR InParanoid; Q03445; -. DR OMA; VEFFCRS; -. DR PhylomeDB; Q03445; -. DR Reactome; R-DME-399710; Activation of AMPA receptors. DR Reactome; R-DME-416993; Trafficking of GluR2-containing AMPA receptors. DR Reactome; R-DME-438066; Unblocking of NMDA receptors, glutamate binding and activation. DR Reactome; R-DME-8849932; Synaptic adhesion-like molecules. DR BioGRID-ORCS; 38742; 0 hits in 3 CRISPR screens. DR GenomeRNAi; 38742; -. DR PRO; PR:Q03445; -. DR Proteomes; UP000000803; Chromosome 3L. DR Bgee; FBgn0004619; Expressed in brain and 19 other tissues. DR Genevisible; Q03445; DM. DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW. DR GO; GO:0008328; C:ionotropic glutamate receptor complex; IC:FlyBase. DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central. DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell. DR GO; GO:0015277; F:kainate selective glutamate receptor activity; IDA:FlyBase. DR GO; GO:0015276; F:ligand-gated ion channel activity; IBA:GO_Central. DR GO; GO:0038023; F:signaling receptor activity; IBA:GO_Central. DR GO; GO:0006812; P:cation transport; IDA:FlyBase. DR InterPro; IPR001828; ANF_lig-bd_rcpt. DR InterPro; IPR019594; Glu/Gly-bd. DR InterPro; IPR001320; Iontro_rcpt. DR InterPro; IPR028082; Peripla_BP_I. DR Pfam; PF01094; ANF_receptor; 1. DR Pfam; PF00060; Lig_chan; 1. DR Pfam; PF10613; Lig_chan-Glu_bd; 1. DR SMART; SM00918; Lig_chan-Glu_bd; 1. DR SMART; SM00079; PBPe; 1. DR SUPFAM; SSF53822; SSF53822; 1. PE 1: Evidence at protein level; KW 3D-structure; Alternative splicing; Cell membrane; Glycoprotein; KW Ion channel; Ion transport; Ligand-gated ion channel; Membrane; KW Postsynaptic cell membrane; Receptor; Reference proteome; Signal; Synapse; KW Transmembrane; Transmembrane helix; Transport. FT SIGNAL 1..27 FT /evidence="ECO:0000255" FT CHAIN 28..991 FT /note="Glutamate receptor 1" FT /id="PRO_0000011555" FT TOPO_DOM 28..611 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 612..632 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 633..710 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 711..731 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 732..895 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 896..916 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 917..991 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT REGION 300..321 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 354..379 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 307..321 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 360..379 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT CARBOHYD 67 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 195 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 208 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 281 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 376 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 385 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 426 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 437 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 477 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT VAR_SEQ 1..265 FT /note="Missing (in isoform 2)" FT /evidence="ECO:0000303|Ref.4" FT /id="VSP_014219" FT CONFLICT 61 FT /note="M -> L (in Ref. 1; AAA28575)" FT /evidence="ECO:0000305" FT CONFLICT 301 FT /note="S -> N (in Ref. 1; AAA28575)" FT /evidence="ECO:0000305" FT CONFLICT 309..316 FT /note="GQSQSQNA -> AKAKARTQ (in Ref. 1; AAA28575)" FT /evidence="ECO:0000305" FT CONFLICT 324 FT /note="A -> P (in Ref. 1; AAA28575)" FT /evidence="ECO:0000305" FT CONFLICT 747 FT /note="T -> A (in Ref. 1; AAA28575)" FT /evidence="ECO:0000305" FT CONFLICT 759 FT /note="Y -> H (in Ref. 1; AAA28575)" FT /evidence="ECO:0000305" FT CONFLICT 916 FT /note="M -> V (in Ref. 1; AAA28575)" FT /evidence="ECO:0000305" FT CONFLICT 944 FT /note="A -> G (in Ref. 1; AAA28575)" FT /evidence="ECO:0000305" FT STRAND 476..484 FT /evidence="ECO:0007829|PDB:5DT6" FT TURN 488..490 FT /evidence="ECO:0007829|PDB:5DT6" FT STRAND 491..493 FT /evidence="ECO:0007829|PDB:5DT6" FT HELIX 504..507 FT /evidence="ECO:0007829|PDB:5DT6" FT STRAND 508..510 FT /evidence="ECO:0007829|PDB:5DT6" FT HELIX 511..523 FT /evidence="ECO:0007829|PDB:5DT6" FT STRAND 526..531 FT /evidence="ECO:0007829|PDB:5DT6" FT HELIX 550..556 FT /evidence="ECO:0007829|PDB:5DT6" FT STRAND 561..563 FT /evidence="ECO:0007829|PDB:5DT6" FT HELIX 571..574 FT /evidence="ECO:0007829|PDB:5DT6" FT STRAND 577..579 FT /evidence="ECO:0007829|PDB:5DT6" FT STRAND 583..586 FT /evidence="ECO:0007829|PDB:5DT6" FT STRAND 588..593 FT /evidence="ECO:0007829|PDB:5DT6" FT HELIX 743..746 FT /evidence="ECO:0007829|PDB:5DT6" FT STRAND 753..755 FT /evidence="ECO:0007829|PDB:5DT6" FT HELIX 761..768 FT /evidence="ECO:0007829|PDB:5DT6" FT HELIX 772..783 FT /evidence="ECO:0007829|PDB:5DT6" FT HELIX 784..787 FT /evidence="ECO:0007829|PDB:5DT6" FT STRAND 788..791 FT /evidence="ECO:0007829|PDB:5DT6" FT HELIX 792..801 FT /evidence="ECO:0007829|PDB:5DT6" FT TURN 802..804 FT /evidence="ECO:0007829|PDB:5DT6" FT STRAND 806..811 FT /evidence="ECO:0007829|PDB:5DT6" FT HELIX 812..819 FT /evidence="ECO:0007829|PDB:5DT6" FT STRAND 826..830 FT /evidence="ECO:0007829|PDB:5DT6" FT STRAND 836..838 FT /evidence="ECO:0007829|PDB:5DT6" FT STRAND 841..843 FT /evidence="ECO:0007829|PDB:5DT6" FT HELIX 849..861 FT /evidence="ECO:0007829|PDB:5DT6" FT HELIX 864..872 FT /evidence="ECO:0007829|PDB:5DT6" FT HELIX 874..876 FT /evidence="ECO:0007829|PDB:5DT6" SQ SEQUENCE 991 AA; 111668 MW; C8917868ABA06F98 CRC64; MHSRLKFLAY LHFICASSIF WPEFSSAQQQ QQTVSLTEKI PLGAIFEQGT DDVQSAFKYA MLNHNLNVSS RRFELQAYVD VINTADAFKL SRLICNQFSR GVYSMLGAVS PDSFDTLHSY SNTFQMPFVT PWFPEKVLAP SSGLLDFAIS MRPDYHQAII DTIQYYGWQS IIYLYDSHDG LLRLQQIYQE LKPGNETFRV QMVKRIANVT MAIEFLHTLE DLGRFSKKRI VLDCPAEMAK EIIVQHVRDI KLGRRTYHYL LSGLVMDNHW PSDVVEFGAI NITGFRIVDS NRRAVRDFHD SRKRLEPSGQ SQSQNAGGPN SLPAISAQAA LMYDAVFVLV EAFNRILRKK PDQFRSNHLQ RRSHGGSSSS SATGTNESSA LLDCNTSKGW VTPWEQGEKI SRVLRKVEID GLSGEIRFDE DGRRINYTLH VVEMSVNSTL QQVAEWRDDA GLLPLHSHNY ASSSRSASAS TGDYDRNHTY IVSSLLEEPY LSLKQYTYGE SLVGNDRFEG YCKDLADMLA AQLGIKYEIR LVQDGNYGAE NQYAPGGWDG MVGELIRKEA DIAISAMTIT AERERVIDFS KPFMTLGISI MIKKPVKQTP GVFSFLNPLS QEIWISVILS YVGVSFVLYF VTRFPPYEWR IVRRPQADST AQQPPGIIGG ATLSEPQAHV PPVPPNEFTM LNSFWYSLAA FMQQGCDITP PSIAGRIAAA VWWFFTIILI SSYTANLAAF LTVERMVAPI KTPEDLTMQT DVNYGTLLYG STWEFFRRSQ IGLHNKMWEY MNANQHHSVH TYDEGIRRVR QSKGKYALLV ESPKNEYVNA RPPCDTMKVG RNIDTKGFGV ATPIGSPLRK RLNEAVLTLK ENGELLRIRN KWWFDKTECN LDQETSTPNE LSLSNVAGIY YILIGGLLLA VIVAIMEFFC RNKTPQLKSP GSNGSAGGVP GMLASSTYQR DSLSDAIMHS QAKLAMQASS EYDERLVGVE LASNVRYQYS M //