ID TFB3_YEAST STANDARD; PRT; 321 AA. AC Q03290; P89104; DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 07-MAR-2006, entry version 39. DE RNA polymerase II transcription factor B subunit 3 (RNA polymerase II DE transcription factor B p38 subunit) (RNA polymerase II transcription DE factor B 38 kDa subunit). GN Name=TFB3; Synonyms=RIG2; OrderedLocusNames=YDR460W; OS Saccharomyces cerevisiae (Baker's yeast). OC Eukaryota; Fungi; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=4932; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 71-85 AND RP 236-250, FUNCTION, SUBUNIT, AND INTERACTION WITH KIN28. RC STRAIN=DBY2019; RX MEDLINE=97382258; PubMed=9235928; DOI=10.1074/jbc.272.31.19319; RA Feaver W.J., Henry N.L., Wang Z., Wu X., Svejstrup J.Q., RA Bushnell D.A., Friedberg E.C., Kornberg R.D.; RT "Genes for Tfb2, Tfb3, and Tfb4 subunits of yeast transcription/repair RT factor IIH. Homology to human cyclin-dependent kinase activating RT kinase and IIH subunits."; RL J. Biol. Chem. 272:19319-19327(1997). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBUNIT. RX PubMed=9294030; DOI=10.1007/s004380050518; RA Faye G., Simon M., Valay J.G., Fesquet D., Facca C.; RT "Rig2, a RING finger protein that interacts with the Kin28/Ccl1 CTD RT kinase in yeast."; RL Mol. Gen. Genet. 255:460-466(1997). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=S288c; RX MEDLINE=97313263; PubMed=9169867; RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., RA Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., RA Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., RA Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., RA Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., RA Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., RA Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., RA Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., RA Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., RA Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., RA Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., RA Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., RA Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., RA Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., RA Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., RA Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., RA Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., RA Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., RA Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., RA Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., RA Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., RA Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., RA Waterston R., Albermann K., Hani J., Heumann K., Kleine K., RA Mewes H.-W., Zollner A., Zaccaria P.; RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."; RL Nature 387:75-78(1997). RN [4] RP SEQUENCE REVISION. RC STRAIN=S288c; RA Sethuraman A., Cherry J.M.; RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases. RN [5] RP SUBUNIT. RX PubMed=11839796; DOI=10.1128/MCB.22.5.1288-1297.2002; RA Keogh M.-C., Cho E.-J., Podolny V., Buratowski S.; RT "Kin28 is found within TFIIH and a Kin28-Ccl1-Tfb3 trimer complex with RT differential sensitivities to T-loop phosphorylation."; RL Mol. Cell. Biol. 22:1288-1297(2002). RN [6] RP SUBUNIT. RX PubMed=14500720; DOI=10.1074/jbc.C300417200; RA Takagi Y., Komori H., Chang W.-H., Hudmon A., Erdjument-Bromage H., RA Tempst P., Kornberg R.D.; RT "Revised subunit structure of yeast transcription factor IIH (TFIIH) RT and reconciliation with human TFIIH."; RL J. Biol. Chem. 278:43897-43900(2003). RN [7] RP LEVEL OF PROTEIN EXPRESSION. RX MEDLINE=22923965; PubMed=14562106; DOI=10.1038/nature02046; RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., RA Dephoure N., O'Shea E.K., Weissman J.S.; RT "Global analysis of protein expression in yeast."; RL Nature 425:737-741(2003). CC -!- FUNCTION: Factor B has CTD kinase activity and DNA-dependent CC ATPase activity, and is essential for polymerase II transcription. CC -!- SUBUNIT: CCL1, KIN28 and TFB3 form the TFIIK complex, a component CC of TFIIH. CC -!- INTERACTION: CC P37366:CCL1; NbExp=1; IntAct=EBI-31406, EBI-4385; CC P06839:RAD3; NbExp=1; IntAct=EBI-31406, EBI-14762; CC -!- SUBCELLULAR LOCATION: Nucleus (By similarity). CC -!- MISCELLANEOUS: Present with 3050 molecules/cell. CC -!- SIMILARITY: Contains 1 RING-type zinc finger. CC -!- CAUTION: Ref.1 sequence differs from that shown due to a CC frameshift in position 262. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U62805; AAB40629.1; ALT_FRAME; Genomic_DNA. DR EMBL; U33050; AAB64899.1; -; Genomic_DNA. DR PIR; S69628; S69628. DR HSSP; P51948; 1G25. DR IntAct; Q03290; -. DR Ensembl; YDR460W; Saccharomyces cerevisiae. DR GenomeReviews; Z71256_GR; YDR460W. DR SGD; S000002868; TFB3. DR LinkHub; Q03290; -. DR GO; GO:0000112; C:nucleotide excision repair factor 3 complex; TAS. DR GO; GO:0005675; C:transcription factor TFIIH complex; IDA. DR GO; GO:0016251; F:general RNA polymerase II transcription fac...; TAS. DR GO; GO:0005515; F:protein binding; IPI. DR GO; GO:0007070; P:negative regulation of transcription from R...; TAS. DR GO; GO:0000717; P:nucleotide-excision repair, DNA duplex unwi...; TAS. DR GO; GO:0006367; P:transcription initiation from RNA polymeras...; TAS. DR InterPro; IPR004575; MAT1. DR InterPro; IPR001841; Znf_RING. DR PANTHER; PTHR12683; MAT1; 1. DR Pfam; PF06391; MAT1; 1. DR TIGRFAMs; TIGR00570; cdk7; 1. DR PROSITE; PS00518; ZF_RING_1; 1. DR PROSITE; PS50089; ZF_RING_2; 1. KW Complete proteome; Direct protein sequencing; Metal-binding; KW Nuclear protein; Transcription; Transcription regulation; Zinc; KW Zinc-finger. FT CHAIN 1 321 RNA polymerase II transcription factor B FT subunit 3. FT /FTId=PRO_0000055942. FT ZN_FING 13 60 RING-type. SQ SEQUENCE 321 AA; 38128 MW; 528D61CF25ADFAF4 CRC64; MLMDEYEENK DMCPICKTDR YLSPDVKFLV NPECYHRICE SCVDRIFSLG PAQCPYKGCD KILRKNKFKT QIFDDVEVEK EVDIRKRVFN VFNKTIDDFN GDLVEYNKYL EEVEDIIYKL DHGIDVAKTE EKLRTYEELN KQLIMNNLER SRTEIESFEQ RQKFEKEMKL KKRLLERQIE EEERMNKEWT KKEIVNRLST TTQDINETIE GVKNTVKLKK SSARRKLEEL NRVLKNNPYF NSNVNVQNSR LKDAVPFTPF NGDREAHPRF TLKGSVYNDP FIKDLEHRKE FIASGFNTNY AYERVLTEAF MGLGCVISEE L //