ID POL2_BAYMG Reviewed; 890 AA. AC Q01365; DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1993, sequence version 1. DT 05-JUN-2019, entry version 69. DE RecName: Full=Genome polyprotein 2; DE Contains: DE RecName: Full=Helper component proteinase; DE Short=HC-pro; DE EC=3.4.22.45; DE Contains: DE RecName: Full=70 kDa protein; GN Name=RNA2; OS Barley yellow mosaic virus (isolate Germany) (BaYMV). OC Viruses; Riboviria; Potyviridae; Bymovirus. OX NCBI_TaxID=31728; OH NCBI_TaxID=4513; Hordeum vulgare (Barley). RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC RNA]. RX PubMed=2016598; DOI=10.1099/0022-1317-72-4-989; RA Davidson A.D., Proels M., Schell J., Steinbiss H.H.; RT "The nucleotide sequence of RNA 2 of barley yellow mosaic virus."; RL J. Gen. Virol. 72:989-993(1991). CC -!- CATALYTIC ACTIVITY: CC Reaction=Hydrolyzes a Gly-|-Gly bond at its own C-terminus, CC commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the CC processing of the potyviral polyprotein.; EC=3.4.22.45; CC -!- PTM: The viral RNA2 of bymoviruses is expressed as a single CC polyprotein which undergoes post-translational proteolytic CC processing resulting in the production of at least two individual CC proteins. The HC-pro cleaves its C-terminus autocatalytically CC (Potential). {ECO:0000305}. CC -!- SIMILARITY: Belongs to the bymoviruses polyprotein 2 family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D01099; BAA00884.1; -; Genomic_RNA. DR PIR; JQ1947; JQ1947. DR SMR; Q01365; -. DR PRIDE; Q01365; -. DR Proteomes; UP000007446; Genome. DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro. DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro. DR Gene3D; 3.90.70.150; -; 1. DR InterPro; IPR001456; HC-pro. DR InterPro; IPR031159; HC_PRO_CPD_dom. DR InterPro; IPR042308; HC_PRO_CPD_sf. DR InterPro; IPR036417; TMV-like_coat_sf. DR Pfam; PF00851; Peptidase_C6; 1. DR SUPFAM; SSF47195; SSF47195; 1. DR PROSITE; PS51744; HC_PRO_CPD; 1. PE 3: Inferred from homology; KW Complete proteome; Hydrolase; Protease; Thiol protease. FT CHAIN 1 255 Helper component proteinase. FT {ECO:0000255}. FT /FTId=PRO_0000040554. FT CHAIN 256 890 70 kDa protein. FT /FTId=PRO_0000040555. FT DOMAIN 135 255 Peptidase C6. {ECO:0000255|PROSITE- FT ProRule:PRU01080}. FT ACT_SITE 143 143 For helper component proteinase activity. FT {ECO:0000255|PROSITE-ProRule:PRU01080}. FT ACT_SITE 215 215 For helper component proteinase activity. FT {ECO:0000255|PROSITE-ProRule:PRU01080}. FT SITE 255 256 Cleavage; by autolysis. FT {ECO:0000255|PROSITE-ProRule:PRU01080}. SQ SEQUENCE 890 AA; 98204 MW; 753AB79EABAD3640 CRC64; MSTSSSRLFF DCGSLDWPNK SLFGDPTTRD VMDEHISSTW NAVIRRHMLA PNADAETILG RDGLPSAQFD AYGAMLPSFI QALNAPTTRL RISAPLSTAE SILCADASHA PWLYMANSVC AYEATHLQPV QTFIAFNFAH GYCYLSLFIP LSFRITPENA RSFSRFLEQL PDILGAYPTL ASLYKTMLFA VRLFPEVLQA PIPIIAKRPG VLQFHVSDAR GLPPSWFPMK CGSVASFIAL ITNNLNSDLL NGIVGSNGDG EHYTNWNSGH NHWIVNRFIT VKDLHSSLKS ALEVDLDTEG GRNAVLDLLL DLGVTNLVRR EKRFPAYFQG AESVYLLLSC ERVGNELVAV QDALQEPLAN YTGKDLRALI INLGGLPSRH PEICYTRNIF ENDNHLVWNF EFYRIASITK NAQIDRDVLS SSMANLFSDF VSESSNGEYR VKEPRPVTQY RVEHDEPVAS GAPSAWWQVL VGITTAILGA IIFFLWRCFL RAKRVKFQAK DSFPWFTTSG DDDLPPPPGD SPSRPPGRSP DRVLPRTVVR DLSFNDDDDL HSVDLNEAGS RFGEVVSLIA RGNLRELAGA IPESLSNLTL LQTSASGSGF YTMVALYLAT LGDAITAFHE HNDASPATIQ SLRTLELQLE ARGLRFNEAG TPANLIQRGV KSSVGRALVR LTQSALLATG ENFRTRMAAT LERIAAERLN TLTAYDQRVI EMTTELLAAI KTALEVERSE LTPHLANAEA LLQVYNNLFS TDYASASLLA LRREMILRSA EGRVGEQPTS ASDAANEELV QRSMTKLDKE IELFQAQIDS QRRAVTITEA SNLRENILQP INTVANIAMA GAFLRGGARH RMPGIPDVAA PMSNPFRAFS GRGHSLTTTR GAGLFRRPRV //