ID SSB_MYCTU Reviewed; 164 AA. AC P9WGD5; L0T5J5; P0A610; P71711; DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot. DT 16-APR-2014, sequence version 1. DT 26-NOV-2014, entry version 8. DE RecName: Full=Single-stranded DNA-binding protein {ECO:0000255|HAMAP-Rule:MF_00984}; DE Short=SSB {ECO:0000255|HAMAP-Rule:MF_00984}; GN Name=ssb; OrderedLocusNames=Rv0054; ORFNames=MTCY21D4.17; OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Corynebacterineae; Mycobacteriaceae; Mycobacterium; OC Mycobacterium tuberculosis complex. OX NCBI_TaxID=83332; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 25618 / H37Rv; RX PubMed=9634230; DOI=10.1038/31159; RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., RA Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, RA Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., RA Connor R., Davies R.M., Devlin K., Feltwell T., Gentles S., Hamlin N., RA Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., RA Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., RA Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., RA Sulston J.E., Taylor K., Whitehead S., Barrell B.G.; RT "Deciphering the biology of Mycobacterium tuberculosis from the RT complete genome sequence."; RL Nature 393:537-544(1998). RN [2] RP CHARACTERIZATION. RX PubMed=10491108; DOI=10.1046/j.1432-1327.1999.00684.x; RA Purnapatre K., Varshney U.; RT "Cloning, over-expression and biochemical characterization of the RT single-stranded DNA binding protein from Mycobacterium tuberculosis."; RL Eur. J. Biochem. 264:591-598(1999). RN [3] RP SSDNA-BINDING. RC STRAIN=ATCC 25618 / H37Rv; RX PubMed=20724443; DOI=10.1093/nar/gkq737; RA Huang F., He Z.G.; RT "Characterization of an interplay between a Mycobacterium tuberculosis RT MazF homolog, Rv1495 and its sole DNA topoisomerase I."; RL Nucleic Acids Res. 38:8219-8230(2010). RN [4] RP CRYSTALLIZATION, AND SUBUNIT. RX PubMed=12888346; DOI=10.1016/S0022-2836(03)00729-0; RA Saikrishnan K., Jeyakanthan J., Venkatesh J., Acharya N., Sekar K., RA Varshney U., Vijayan M.; RT "Structure of Mycobacterium tuberculosis single-stranded DNA-binding RT protein. Variability in quaternary structure and its implications."; RL J. Mol. Biol. 331:385-393(2003). RN [5] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC STRAIN=ATCC 25618 / H37Rv; RX PubMed=21969609; DOI=10.1074/mcp.M111.011627; RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., RA Yadav A.K., Shrivastava P., Marimuthu A., Anand S., Sundaram H., RA Kingsbury R., Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., RA Katoch K., Katoch V.M., Kumar P., Chaerkady R., Ramachandran S., RA Dash D., Pandey A.; RT "Proteogenomic analysis of Mycobacterium tuberculosis by high RT resolution mass spectrometry."; RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011). CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00984, CC ECO:0000269|PubMed:12888346}. CC -!- SIMILARITY: Contains 1 SSB domain. {ECO:0000255|HAMAP- CC Rule:MF_00984}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AL123456; CCP42776.1; -; Genomic_DNA. DR PIR; F70913; F70913. DR RefSeq; NP_214568.1; NC_000962.3. DR RefSeq; YP_006513368.1; NC_018143.2. DR PDB; 1UE1; X-ray; 2.50 A; A/B=1-164. DR PDB; 1UE5; X-ray; 2.60 A; A/B=1-164. DR PDB; 1UE6; X-ray; 2.70 A; A/B/C/D=1-164. DR PDB; 1UE7; X-ray; 3.20 A; A/B/C/D=1-164. DR PDBsum; 1UE1; -. DR PDBsum; 1UE5; -. DR PDBsum; 1UE6; -. DR PDBsum; 1UE7; -. DR ProteinModelPortal; P9WGD5; -. DR SMR; P9WGD5; 2-123. DR GeneID; 13316032; -. DR GeneID; 887013; -. DR KEGG; mtu:Rv0054; -. DR KEGG; mtv:RVBD_0054; -. DR TubercuList; Rv0054; -. DR KO; K03111; -. DR PhylomeDB; P9WGD5; -. DR GO; GO:0005576; C:extracellular region; IDA:MTBBASE. DR GO; GO:0005886; C:plasma membrane; IDA:MTBBASE. DR GO; GO:0003697; F:single-stranded DNA binding; IDA:MTBBASE. DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:MTBBASE. DR GO; GO:0006260; P:DNA replication; IEA:InterPro. DR GO; GO:0051289; P:protein homotetramerization; IPI:MTBBASE. DR GO; GO:0046677; P:response to antibiotic; IEP:MTBBASE. DR Gene3D; 2.40.50.140; -; 1. DR HAMAP; MF_00984; SSB; 1. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR000424; Primosome_PriB/ssb. DR InterPro; IPR011344; ssDNA-bd. DR PANTHER; PTHR10302; PTHR10302; 1. DR Pfam; PF00436; SSB; 1. DR PIRSF; PIRSF002070; SSB; 1. DR SUPFAM; SSF50249; SSF50249; 1. DR TIGRFAMs; TIGR00621; ssb; 1. DR PROSITE; PS50935; SSB; 1. PE 1: Evidence at protein level; KW 3D-structure; Complete proteome; DNA-binding; Reference proteome. FT CHAIN 1 164 Single-stranded DNA-binding protein. FT /FTId=PRO_0000096069. FT DOMAIN 1 110 SSB. {ECO:0000255|HAMAP-Rule:MF_00984}. FT STRAND 6 15 {ECO:0000244|PDB:1UE1}. FT STRAND 18 21 {ECO:0000244|PDB:1UE1}. FT TURN 23 25 {ECO:0000244|PDB:1UE7}. FT STRAND 27 35 {ECO:0000244|PDB:1UE1}. FT STRAND 53 60 {ECO:0000244|PDB:1UE1}. FT HELIX 61 70 {ECO:0000244|PDB:1UE1}. FT STRAND 76 88 {ECO:0000244|PDB:1UE1}. FT STRAND 90 94 {ECO:0000244|PDB:1UE1}. FT STRAND 96 108 {ECO:0000244|PDB:1UE1}. FT STRAND 111 116 {ECO:0000244|PDB:1UE1}. SQ SEQUENCE 164 AA; 17353 MW; 14797F8379FC2036 CRC64; MAGDTTITIV GNLTADPELR FTPSGAAVAN FTVASTPRIY DRQTGEWKDG EALFLRCNIW REAAENVAES LTRGARVIVS GRLKQRSFET REGEKRTVIE VEVDEIGPSL RYATAKVNKA SRSGGFGSGS RPAPAQTSSA SGDDPWGSAP ASGSFGGGDD EPPF //