ID SSB_MYCTU Reviewed; 164 AA. AC P9WGD5; L0T5J5; P0A610; P71711; DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot. DT 16-APR-2014, sequence version 1. DT 07-APR-2021, entry version 38. DE RecName: Full=Single-stranded DNA-binding protein {ECO:0000255|HAMAP-Rule:MF_00984}; DE Short=SSB {ECO:0000255|HAMAP-Rule:MF_00984}; GN Name=ssb; OrderedLocusNames=Rv0054; ORFNames=MTCY21D4.17; OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; OC Mycobacterium; Mycobacterium tuberculosis complex. OX NCBI_TaxID=83332; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 25618 / H37Rv; RX PubMed=9634230; DOI=10.1038/31159; RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., RA Barrell B.G.; RT "Deciphering the biology of Mycobacterium tuberculosis from the complete RT genome sequence."; RL Nature 393:537-544(1998). RN [2] RP CHARACTERIZATION. RX PubMed=10491108; DOI=10.1046/j.1432-1327.1999.00684.x; RA Purnapatre K., Varshney U.; RT "Cloning, over-expression and biochemical characterization of the single- RT stranded DNA binding protein from Mycobacterium tuberculosis."; RL Eur. J. Biochem. 264:591-598(1999). RN [3] RP SSDNA-BINDING. RC STRAIN=ATCC 25618 / H37Rv; RX PubMed=20724443; DOI=10.1093/nar/gkq737; RA Huang F., He Z.G.; RT "Characterization of an interplay between a Mycobacterium tuberculosis MazF RT homolog, Rv1495 and its sole DNA topoisomerase I."; RL Nucleic Acids Res. 38:8219-8230(2010). RN [4] RP CRYSTALLIZATION, AND SUBUNIT. RX PubMed=12888346; DOI=10.1016/s0022-2836(03)00729-0; RA Saikrishnan K., Jeyakanthan J., Venkatesh J., Acharya N., Sekar K., RA Varshney U., Vijayan M.; RT "Structure of Mycobacterium tuberculosis single-stranded DNA-binding RT protein. Variability in quaternary structure and its implications."; RL J. Mol. Biol. 331:385-393(2003). RN [5] RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. RC STRAIN=ATCC 25618 / H37Rv; RX PubMed=21969609; DOI=10.1074/mcp.m111.011627; RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K., RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R., RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M., RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.; RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution RT mass spectrometry."; RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011). CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00984, CC ECO:0000269|PubMed:12888346}. CC -!- INTERACTION: CC P9WGD5; P9WMR3: dnaB; NbExp=3; IntAct=EBI-9081374, EBI-9081402; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AL123456; CCP42776.1; -; Genomic_DNA. DR PIR; F70913; F70913. DR RefSeq; NP_214568.1; NC_000962.3. DR RefSeq; WP_003400534.1; NZ_NVQJ01000005.1. DR PDB; 1UE1; X-ray; 2.50 A; A/B=1-164. DR PDB; 1UE5; X-ray; 2.60 A; A/B=1-164. DR PDB; 1UE6; X-ray; 2.70 A; A/B/C/D=1-164. DR PDB; 1UE7; X-ray; 3.20 A; A/B/C/D=1-164. DR PDBsum; 1UE1; -. DR PDBsum; 1UE5; -. DR PDBsum; 1UE6; -. DR PDBsum; 1UE7; -. DR SMR; P9WGD5; -. DR IntAct; P9WGD5; 1. DR MINT; P9WGD5; -. DR STRING; 83332.Rv0054; -. DR PaxDb; P9WGD5; -. DR GeneID; 887013; -. DR KEGG; mtu:Rv0054; -. DR TubercuList; Rv0054; -. DR eggNOG; COG0629; Bacteria. DR OMA; ASGFDDM; -. DR PhylomeDB; P9WGD5; -. DR Proteomes; UP000001584; Chromosome. DR GO; GO:0005576; C:extracellular region; HDA:MTBBASE. DR GO; GO:0009295; C:nucleoid; IBA:GO_Central. DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE. DR GO; GO:0003697; F:single-stranded DNA binding; IDA:MTBBASE. DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:MTBBASE. DR GO; GO:0006260; P:DNA replication; IEA:InterPro. DR GO; GO:0051096; P:positive regulation of helicase activity; IBA:GO_Central. DR GO; GO:0046677; P:response to antibiotic; IEP:MTBBASE. DR CDD; cd04496; SSB_OBF; 1. DR HAMAP; MF_00984; SSB; 1. DR InterPro; IPR012340; NA-bd_OB-fold. DR InterPro; IPR000424; Primosome_PriB/ssb. DR InterPro; IPR011344; ssDNA-bd. DR PANTHER; PTHR10302; PTHR10302; 1. DR Pfam; PF00436; SSB; 1. DR SUPFAM; SSF50249; SSF50249; 1. DR TIGRFAMs; TIGR00621; ssb; 1. DR PROSITE; PS50935; SSB; 1. PE 1: Evidence at protein level; KW 3D-structure; DNA-binding; Reference proteome. FT CHAIN 1..164 FT /note="Single-stranded DNA-binding protein" FT /id="PRO_0000096069" FT DOMAIN 1..110 FT /note="SSB" FT /evidence="ECO:0000255|HAMAP-Rule:MF_00984" FT STRAND 6..15 FT /evidence="ECO:0007744|PDB:1UE1" FT STRAND 18..21 FT /evidence="ECO:0007744|PDB:1UE1" FT TURN 23..25 FT /evidence="ECO:0007744|PDB:1UE7" FT STRAND 27..35 FT /evidence="ECO:0007744|PDB:1UE1" FT STRAND 42..45 FT /evidence="ECO:0007744|PDB:1UE1" FT STRAND 53..60 FT /evidence="ECO:0007744|PDB:1UE1" FT HELIX 61..70 FT /evidence="ECO:0007744|PDB:1UE1" FT STRAND 76..88 FT /evidence="ECO:0007744|PDB:1UE1" FT STRAND 90..94 FT /evidence="ECO:0007744|PDB:1UE1" FT STRAND 96..108 FT /evidence="ECO:0007744|PDB:1UE1" FT STRAND 111..116 FT /evidence="ECO:0007744|PDB:1UE1" SQ SEQUENCE 164 AA; 17353 MW; 14797F8379FC2036 CRC64; MAGDTTITIV GNLTADPELR FTPSGAAVAN FTVASTPRIY DRQTGEWKDG EALFLRCNIW REAAENVAES LTRGARVIVS GRLKQRSFET REGEKRTVIE VEVDEIGPSL RYATAKVNKA SRSGGFGSGS RPAPAQTSSA SGDDPWGSAP ASGSFGGGDD EPPF //