ID PGL3_ARATH Reviewed; 626 AA. AC P92990; O64599; Q0WV83; Q8H7B8; Q93ZQ4; DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot. DT 22-NOV-2005, sequence version 2. DT 03-MAY-2023, entry version 115. DE RecName: Full=Polygalacturonase 1 beta-like protein 3 {ECO:0000303|PubMed:26106400}; DE Short=AtPGL3 {ECO:0000303|PubMed:26106400}; DE AltName: Full=Aromatic-rich glycoprotein JP650; DE AltName: Full=PG1beta-like protein 3 {ECO:0000303|PubMed:26106400}; DE AltName: Full=Polygalacturonase 2 {ECO:0000303|PubMed:19639386}; DE AltName: Full=Probable polygalacturonase non-catalytic subunit JP650; DE Flags: Precursor; GN Name=PGL3 {ECO:0000303|PubMed:26106400}; GN Synonyms=JP650, PG2 {ECO:0000303|PubMed:19639386}; GN OrderedLocusNames=At1g70370 {ECO:0000312|Araport:AT1G70370}; GN ORFNames=F17O7.9 {ECO:0000312|EMBL:AAC18803.1}; OS Arabidopsis thaliana (Mouse-ear cress). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RA Watson C.F., Schuchman B., Liu J., DellaPenna D.; RT "Arabidopsis aromatic rich glycoprotein JP650."; RL Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. Columbia; RX PubMed=11130712; DOI=10.1038/35048500; RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., RA Yu G., Fraser C.M., Venter J.C., Davis R.W.; RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."; RL Nature 408:816-820(2000). RN [3] RP GENOME REANNOTATION. RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference RT genome."; RL Plant J. 89:789-804(2017). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RX PubMed=14593172; DOI=10.1126/science.1088305; RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., RA Ecker J.R.; RT "Empirical analysis of transcriptional activity in the Arabidopsis RT genome."; RL Science 302:842-846(2003). RN [5] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC STRAIN=cv. Columbia; RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K., RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., RA Shinozaki K.; RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."; RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases. RN [6] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-226. RA Stracke R., Palme K.; RT "Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves RT and guard cells."; RL Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases. RN [7] RP DOMAIN. RX PubMed=9790599; DOI=10.1007/s004380050832; RA Hattori J., Boutilier K.A., van Lookeren Campagne M.M., Miki B.L.; RT "A conserved BURP domain defines a novel group of plant proteins with RT unusual primary structures."; RL Mol. Gen. Genet. 259:424-428(1998). RN [8] RP GENE FAMILY, AND NOMENCLATURE. RX PubMed=19639386; DOI=10.1007/s11103-009-9526-6; RA Van Son L., Tiedemann J., Rutten T., Hillmer S., Hinz G., Zank T., RA Manteuffel R., Baeumlein H.; RT "The BURP domain protein AtUSPL1 of Arabidopsis thaliana is destined to the RT protein storage vacuoles and overexpression of the cognate gene distorts RT seed development."; RL Plant Mol. Biol. 71:319-329(2009). RN [9] RP FUNCTION, GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY, DEVELOPMENTAL RP STAGE, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND DOMAIN. RX PubMed=26106400; DOI=10.3389/fpls.2015.00412; RA Park J., Cui Y., Kang B.H.; RT "AtPGL3 is an Arabidopsis BURP domain protein that is localized to the cell RT wall and promotes cell enlargement."; RL Front. Plant Sci. 6:412-412(2015). CC -!- FUNCTION: Involved in cell size determination. May serve as a chaperone CC for expansins through the secretory pathway. CC {ECO:0000269|PubMed:26106400}. CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast CC {ECO:0000269|PubMed:26106400}. Secreted, cell wall CC {ECO:0000269|PubMed:26106400}. CC -!- TISSUE SPECIFICITY: Expressed in flowers and stems. Detected in CC trichomes, guard cells, root vascular tissue, root hairs, pollen sacs, CC sepals and styles of pistils. {ECO:0000269|PubMed:26106400}. CC -!- DEVELOPMENTAL STAGE: Barely detectable in 6 days after-germination CC (DAG) seedlings, but highly expressed in 14 DAG seedlings. CC {ECO:0000269|PubMed:26106400}. CC -!- DOMAIN: The BURP domain located at the C-terminus has not been CC identified in non-plant proteins (PubMed:9790599). It is critical for CC PGL3's role in cell growth (PubMed:26106400). CC {ECO:0000269|PubMed:26106400, ECO:0000269|PubMed:9790599}. CC -!- DISRUPTION PHENOTYPE: Slightly reduced size of the plant. Atpgl1, CC atpgl2 and atpgl3 triple mutants produce smaller leaves and petioles. CC {ECO:0000269|PubMed:26106400}. CC -!- MISCELLANEOUS: Unlike the tomato GP1, the BURP domain of AtPGL3 is not CC cleaved when the protein is secreted to the cell wall. CC {ECO:0000269|PubMed:26106400}. CC -!- SEQUENCE CAUTION: CC Sequence=AAL08244.1; Type=Frameshift; Evidence={ECO:0000305}; CC Sequence=AAN18083.1; Type=Frameshift; Evidence={ECO:0000305}; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U63373; AAB39546.1; -; mRNA. DR EMBL; AC003671; AAC18803.1; -; Genomic_DNA. DR EMBL; CP002684; AEE35050.1; -; Genomic_DNA. DR EMBL; CP002684; AEE35051.1; -; Genomic_DNA. DR EMBL; AY056388; AAL08244.1; ALT_FRAME; mRNA. DR EMBL; BT000514; AAN18083.1; ALT_FRAME; mRNA. DR EMBL; AK226888; BAE98965.1; -; mRNA. DR EMBL; AF083752; AAN60310.1; -; mRNA. DR PIR; T01485; T01485. DR RefSeq; NP_001185361.1; NM_001198432.1. DR RefSeq; NP_177194.1; NM_105705.4. DR AlphaFoldDB; P92990; -. DR STRING; 3702.AT1G70370.1; -. DR GlyCosmos; P92990; 6 sites, No reported glycans. DR PaxDb; P92990; -. DR ProteomicsDB; 236724; -. DR EnsemblPlants; AT1G70370.1; AT1G70370.1; AT1G70370. DR EnsemblPlants; AT1G70370.2; AT1G70370.2; AT1G70370. DR GeneID; 843373; -. DR Gramene; AT1G70370.1; AT1G70370.1; AT1G70370. DR Gramene; AT1G70370.2; AT1G70370.2; AT1G70370. DR KEGG; ath:AT1G70370; -. DR Araport; AT1G70370; -. DR TAIR; locus:2016194; AT1G70370. DR eggNOG; ENOG502QT2V; Eukaryota. DR HOGENOM; CLU_011822_5_0_1; -. DR InParanoid; P92990; -. DR OMA; NYGNARA; -. DR OrthoDB; 1208871at2759; -. DR PhylomeDB; P92990; -. DR PRO; PR:P92990; -. DR Proteomes; UP000006548; Chromosome 1. DR ExpressionAtlas; P92990; baseline and differential. DR Genevisible; P92990; AT. DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell. DR GO; GO:0009505; C:plant-type cell wall; IDA:TAIR. DR GO; GO:0042547; P:cell wall modification involved in multidimensional cell growth; IMP:TAIR. DR GO; GO:0009827; P:plant-type cell wall modification; IMP:TAIR. DR InterPro; IPR004873; BURP_dom. DR PANTHER; PTHR31458:SF10; POLYGALACTURONASE 1 BETA-LIKE PROTEIN 1-RELATED; 1. DR PANTHER; PTHR31458; POLYGALACTURONASE 1 BETA-LIKE PROTEIN 2; 1. DR Pfam; PF03181; BURP; 1. DR SMART; SM01045; BURP; 1. DR PROSITE; PS51277; BURP; 1. PE 2: Evidence at transcript level; KW Apoplast; Cell wall; Glycoprotein; Reference proteome; Repeat; Secreted; KW Signal. FT SIGNAL 1..23 FT /evidence="ECO:0000255" FT CHAIN 24..626 FT /note="Polygalacturonase 1 beta-like protein 3" FT /id="PRO_0000042954" FT REPEAT 117..120 FT /note="FXXY 1" FT /evidence="ECO:0000305" FT REPEAT 125..128 FT /note="FXXY 2" FT /evidence="ECO:0000305" FT REPEAT 139..142 FT /note="FXXY 3" FT /evidence="ECO:0000305" FT REPEAT 153..156 FT /note="FXXY 4" FT /evidence="ECO:0000305" FT REPEAT 167..170 FT /note="FXXY 5" FT /evidence="ECO:0000305" FT REPEAT 181..184 FT /note="FXXY 6" FT /evidence="ECO:0000305" FT REPEAT 195..198 FT /note="FXXY 7" FT /evidence="ECO:0000305" FT REPEAT 209..212 FT /note="FXXY 8" FT /evidence="ECO:0000305" FT REPEAT 223..226 FT /note="FXXY 9" FT /evidence="ECO:0000305" FT REPEAT 238..241 FT /note="FXXY 10" FT /evidence="ECO:0000305" FT REPEAT 252..255 FT /note="FXXY 11" FT /evidence="ECO:0000305" FT REPEAT 266..269 FT /note="FXXY 12" FT /evidence="ECO:0000305" FT REPEAT 280..283 FT /note="FXXY 13" FT /evidence="ECO:0000305" FT REPEAT 294..297 FT /note="FXXY 14" FT /evidence="ECO:0000305" FT REPEAT 308..311 FT /note="FXXY 15" FT /evidence="ECO:0000305" FT REPEAT 322..325 FT /note="FXXY 16" FT /evidence="ECO:0000305" FT REPEAT 336..339 FT /note="FXXY 17" FT /evidence="ECO:0000305" FT REPEAT 350..353 FT /note="FXXY 18" FT /evidence="ECO:0000305" FT REPEAT 364..367 FT /note="FXXY 19" FT /evidence="ECO:0000305" FT REPEAT 373..376 FT /note="FXXY 20" FT /evidence="ECO:0000305" FT REPEAT 383..386 FT /note="FXXY 21" FT /evidence="ECO:0000305" FT DOMAIN 411..625 FT /note="BURP" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00604" FT CARBOHYD 124 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 141 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 277 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 370 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 387 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CARBOHYD 465 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT CONFLICT 70 FT /note="A -> S (in Ref. 6; AAN60310)" FT /evidence="ECO:0000305" FT CONFLICT 242 FT /note="G -> A (in Ref. 1; AAB39546)" FT /evidence="ECO:0000305" SQ SEQUENCE 626 AA; 68060 MW; F324231B27D4B710 CRC64; MLKQFLLLQS FSFFLFNVVI VGGRTFGGGF SAEENPFTPK ASLVRYWNKE IRGQSPRSEF LISKASPLNA VDSATFSKLA AANSLPTRFP DFCSAANLFC FPDLGASLEK HDDDVKFSVY DQKNFTNYGN ARAGGADSFK NYSKDGNVVT DSFRRYSRNA AGHDDKFTVY GENSNVVEEG FNSYGTFGTG GAGDFTNYQN NVNNPTSRFT AYSDGGNGRS QTFKTYTHEA NAGNGQSFTS YGKNGNGVPN EFTSYGVSSN VIGSGFSNYG ESGNAANDTF TSYGSDGNVP QNNFNNYGAS GNAAVDTFAN YRDKANVGDD SFSSYAKDSN SEKVNFVNYG QSFNPGSETF TGYGKGAEGS KLSFKTYTPN STFKDYAKKG VAFAKYNVST TTANTVGDGK TVNKWIEPGK FFRESSLKEG TVIPMPDIKD KMPKRSFLPR SIITKLPFST SKLGEIKRIF HAVENSTMGG IITDAVTECE RPPSVGETKR CVGSAEDMID FATSVLGRSV VLRTTENVAG SKEKVVIGKV NGINGGKLTK AVSCHQSLYP YLLYYCHSVP KVRVYEADLL ELNSKKKINH GIAICHMDTS SWGPSHGAFL ALGSKPGRIE VCHWIFENDM NWAIAD //