ID AP2A_DROME STANDARD; PRT; 940 AA. AC P91926; O01937; Q86P64; Q9VPP4; DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-1997, sequence version 1. DT 04-APR-2006, entry version 42. DE AP-2 complex subunit alpha (Alpha-adaptin). GN Name=alpha-Adaptin; ORFNames=CG4260; OS Drosophila melanogaster (Fruit fly). OC Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; OC Ephydroidea; Drosophilidae; Drosophila. OX NCBI_TaxID=7227; RN [1] RP NUCLEOTIDE SEQUENCE. RC STRAIN=Canton-S; TISSUE=Embryo; RX MEDLINE=97236497; PubMed=9118220; DOI=10.1016/S0092-8674(00)81923-6; RA Gonzalez-Gaitan M.A., Jaeckle H.; RT "Role of Drosophila alpha-adaptin in presynaptic vesicle recycling."; RL Cell 88:767-776(1997). RN [2] RP NUCLEOTIDE SEQUENCE. RC TISSUE=Embryo; RX MEDLINE=97431776; PubMed=9285813; RA Dornan S.C., Jackson A.P., Gay N.J.; RT "Alpha-adaptin, a marker for endocytosis, is expressed in complex RT patterns during Drosophila development."; RL Mol. Biol. Cell 8:1391-1403(1997). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Berkeley; RX MEDLINE=20196006; PubMed=10731132; DOI=10.1126/science.287.5461.2185; RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., RA Kimmel B.E., Kodira C.D., Kraft C., Kravitz S., Kulp D., Lai Z., RA Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S., Zhu X., Smith H.O., RA Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.; RT "The genome sequence of Drosophila melanogaster."; RL Science 287:2185-2195(2000). RN [4] RP GENOME REANNOTATION. RX MEDLINE=22426069; PubMed=12537572; RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., RA Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., RA Lewis S.E.; RT "Annotation of the Drosophila melanogaster euchromatic genome: a RT systematic review."; RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002). RN [5] RP NUCLEOTIDE SEQUENCE OF 93-940. RC STRAIN=Berkeley; TISSUE=Head; RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., RA Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., RA George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., RA Miranda A., Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., RA Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., RA Celniker S.E.; RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Adaptins are components of the adapter complexes which CC link clathrin to receptors in coated vesicles. Clathrin-associated CC protein complexes are believed to interact with the cytoplasmic CC tails of membrane proteins, leading to their selection and CC concentration. Alpha adaptin is a subunit of the plasma membrane CC adapter (By similarity). CC -!- SUBUNIT: Assembly protein complex 2 (AP-2) is a heterotetramer CC composed of two large chains (alpha and beta), a medium chain CC (mu/AP50) and a small chain (sigma/AP17). CC -!- SUBCELLULAR LOCATION: Component of the coat surrounding the CC cytoplasmic face of coated vesicles in the plasma membrane (By CC similarity). CC -!- SIMILARITY: Belongs to the adaptor complexes large subunit family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Y11104; CAA71991.1; -; mRNA. DR EMBL; Y13092; CAA73533.1; -; mRNA. DR EMBL; AE003589; AAF56103.2; -; Genomic_DNA. DR EMBL; BT003458; AAO39461.1; ALT_INIT; mRNA. DR UniGene; Dm.246; -. DR UniGene; Dm.28224; -. DR HSSP; P17427; 1GW5. DR Ensembl; CG4260; Drosophila melanogaster. DR FlyBase; FBgn0015567; alpha-Adaptin. DR BioCyc; DMEL-XXX-02:DMEL-XXX-02-006702-MONOMER; -. DR GO; GO:0030135; C:coated vesicle; TAS. DR GO; GO:0005886; C:plasma membrane; IDA. DR GO; GO:0008021; C:synaptic vesicle; TAS. DR GO; GO:0008356; P:asymmetric cell division; TAS. DR GO; GO:0007269; P:neurotransmitter secretion; NAS. DR GO; GO:0016183; P:synaptic vesicle coating; TAS. DR GO; GO:0048489; P:synaptic vesicle transport; TAS. DR InterPro; IPR008152; A/G_adapt_C. DR InterPro; IPR002553; Adaptin_N. DR InterPro; IPR003164; AP2_A_adaptin_C. DR InterPro; IPR009028; AP2_adap_app. DR InterPro; IPR011989; ARM-like. DR Pfam; PF01602; Adaptin_N; 1. DR Pfam; PF02296; Alpha_adaptin_C; 1. DR Pfam; PF02883; Alpha_adaptinC2; 1. KW Coated pits; Complete proteome. FT CHAIN 1 940 AP-2 complex subunit alpha. FT /FTId=PRO_0000193735. FT COMPBIAS 644 715 Asn-rich. FT CONFLICT 207 207 T -> A (in Ref. 5). FT CONFLICT 325 325 N -> E (in Ref. 2). FT CONFLICT 489 489 A -> P (in Ref. 2). FT CONFLICT 587 587 S -> N (in Ref. 2). FT CONFLICT 708 708 Missing (in Ref. 2). FT CONFLICT 711 712 LF -> VS (in Ref. 2). FT CONFLICT 722 723 EM -> GK (in Ref. 2). FT CONFLICT 741 743 LFY -> FSN (in Ref. 2). FT CONFLICT 877 877 Q -> P (in Ref. 2). SQ SEQUENCE 940 AA; 105620 MW; F745045CD2C1D9C5 CRC64; MAPVRGDGMR GLAVFISDIR NCKSKEAEVK RINKELANIR SKFKGDKTLD GYQKKKYVCK LLFIFLLGHD IDFGHMEAVN LLSSNKYSEK QIGYLFISVL VNTNSDLIRL IIQSIKNDLQ SRNPVHVNLA LQCIANIGSR DMAESFSNEI PKLLVSGDTM DVVKQSAALC LLRLFRSSPD IIPGGEWTSR IIHLLNDQHM GVVTAATSLI DALVKRNPDE YKGCVNLAVS RLSRIVTASY TDLQDYTYYF VPAPWLSVKL LRLLQNYNPV TEEAGVRARL NETLETILNK AQEPPKSKKV QHSNAKNAVL FEAINLIIHS DSEPNLLVRA CNQLGQFLSN RETNLRYLAL ESMCHLATSE FSHEEVKKHQ EVVILSMKME KDVSVRQMAV DLLYAMCDRG NAEEIVQEML NYLETADYSI REEMVLKVAI LAEKYATDYT WYVDVILNLI RIAGDYVSEE VWYRVIQIVI NREEVQGYAA KTVFEALQAP ACHENMVKVG GYILGEFGNL IAGDSRSAPL VQFKLLHSKY HLCSPMTRAL LLSTYIKFIN LFPEIRTNIQ DVFRQHSNLR SADAELQQRA SEYLQLSIVA STDVLATVLE EMPSFPERES SILAVLKKKK PGRVPENEIR ESKSPAPLTS AAQNNALVNN SHSKLNNSNA NTDLLGLSTP PSNNIGSGSN SNSTLIDVLG DMYGSNSNNN SSAVYNTKKF LFKNNGVLFE NEMLQIGVKS EFRQNLGRLG LFYGNKTQVP LTNFNPVLQW SAEDALKLNV QMKVVEPTLE AGAQIQQLLT AECIEDYADA PTIEISFRYN GTQQKFSIKL PLSVNKFFEP TEMNAESFFA RWKNLSGEQQ RSQKVFKAAQ PLDLPGARNK LMGFGMQLLD QVDPNPDNMV CAGIIHTQSQ QVGCLMRLEP NKQAQMFRLT VRASKETVTR EICDLLTDQF //