ID PRVB1_MERHU Reviewed; 108 AA. AC P86764; DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot. DT 05-OCT-2010, sequence version 1. DT 14-DEC-2022, entry version 27. DE RecName: Full=Parvalbumin beta 1 {ECO:0000303|PubMed:20586483}; OS Merluccius hubbsi (Argentine hake) (Merluccius gayi). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata; OC Zeiogadaria; Gadariae; Gadiformes; Gadoidei; Merlucciidae; Merluccius. OX NCBI_TaxID=89949; RN [1] {ECO:0000305} RP PROTEIN SEQUENCE, MASS SPECTROMETRY, AND ACETYLATION AT ALA-1. RC TISSUE=Muscle {ECO:0000269|PubMed:20586483}; RX PubMed=20586483; DOI=10.1021/pr100163e; RA Carrera M., Canas B., Vazquez J., Gallardo J.M.; RT "Extensive de novo sequencing of new parvalbumin isoforms using a novel RT combination of bottom-up proteomics, accurate molecular mass measurement by RT FTICR-MS, and selected MS/MS ion monitoring."; RL J. Proteome Res. 9:4393-4406(2010). CC -!- FUNCTION: In muscle, parvalbumin is thought to be involved in CC relaxation after contraction. It binds two calcium ions (By CC similarity). {ECO:0000250|UniProtKB:P02622, CC ECO:0000250|UniProtKB:P02624}. CC -!- MASS SPECTROMETRY: Mass=11329.748; Mass_error=0.006; CC Method=Electrospray; Evidence={ECO:0000269|PubMed:20586483}; CC -!- MISCELLANEOUS: Is regarded as an important allergen. CC {ECO:0000250|UniProtKB:P02622, ECO:0000269|PubMed:20586483}. CC -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is: CC 4.57, its MW is: 11.30 kDa. {ECO:0000269|PubMed:20586483}. CC -!- SIMILARITY: Belongs to the parvalbumin family. {ECO:0000255}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR AlphaFoldDB; P86764; -. DR SMR; P86764; -. DR Allergome; 7644; Mer hu 1. DR iPTMnet; P86764; -. DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro. DR InterPro; IPR011992; EF-hand-dom_pair. DR InterPro; IPR018247; EF_Hand_1_Ca_BS. DR InterPro; IPR002048; EF_hand_dom. DR InterPro; IPR008080; Parvalbumin. DR PANTHER; PTHR11653; PARVALBUMIN ALPHA; 1. DR Pfam; PF13499; EF-hand_7; 1. DR SUPFAM; SSF47473; EF-hand; 1. DR PROSITE; PS00018; EF_HAND_1; 2. DR PROSITE; PS50222; EF_HAND_2; 2. PE 1: Evidence at protein level; KW Acetylation; Allergen; Calcium; Direct protein sequencing; Metal-binding; KW Muscle protein; Repeat. FT CHAIN 1..108 FT /note="Parvalbumin beta 1" FT /id="PRO_0000399422" FT DOMAIN 38..73 FT /note="EF-hand 1" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448" FT DOMAIN 77..108 FT /note="EF-hand 2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448" FT BINDING 51 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="1" FT /evidence="ECO:0000250|UniProtKB:P02621, FT ECO:0000255|PROSITE-ProRule:PRU00448" FT BINDING 53 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="1" FT /evidence="ECO:0000250|UniProtKB:P02621, FT ECO:0000255|PROSITE-ProRule:PRU00448" FT BINDING 55 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="1" FT /evidence="ECO:0000250|UniProtKB:P02621, FT ECO:0000255|PROSITE-ProRule:PRU00448" FT BINDING 57 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="1" FT /evidence="ECO:0000250|UniProtKB:P02621" FT BINDING 59 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="1" FT /evidence="ECO:0000250|UniProtKB:P02621" FT BINDING 62 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="1" FT /evidence="ECO:0000250|UniProtKB:P02621, FT ECO:0000255|PROSITE-ProRule:PRU00448" FT BINDING 90 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="2" FT /evidence="ECO:0000250|UniProtKB:P02621, FT ECO:0000255|PROSITE-ProRule:PRU00448" FT BINDING 92 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="2" FT /evidence="ECO:0000250|UniProtKB:P02621, FT ECO:0000255|PROSITE-ProRule:PRU00448" FT BINDING 94 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="2" FT /evidence="ECO:0000250|UniProtKB:P02621, FT ECO:0000255|PROSITE-ProRule:PRU00448" FT BINDING 96 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448" FT BINDING 101 FT /ligand="Ca(2+)" FT /ligand_id="ChEBI:CHEBI:29108" FT /ligand_label="2" FT /evidence="ECO:0000250|UniProtKB:P02621, FT ECO:0000255|PROSITE-ProRule:PRU00448" FT MOD_RES 1 FT /note="N-acetylalanine" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 5 FT /note="I or L" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 6 FT /note="L or I" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 11 FT /note="I or L" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 15 FT /note="L or I" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 19 FT /note="K or Q" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 25 FT /note="K or Q" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 32 FT /note="K or Q" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 33 FT /note="I or L" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 35 FT /note="L or I" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 36 FT /note="K or Q" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 38 FT /note="K or Q" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 43 FT /note="I or L" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 44 FT /note="K or Q" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 45 FT /note="K or Q" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 49 FT /note="I or L" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 50 FT /note="I or L" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 52 FT /note="Q or K" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 54 FT /note="K or Q" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 63 FT /note="L or I" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 64 FT /note="K or Q" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 65 FT /note="L or I" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 67 FT /note="L or I" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 68 FT /note="Q or K" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 77 FT /note="L or I" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 86 FT /note="L or I" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 87 FT /note="K or Q" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 96 FT /note="K or Q" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 97 FT /note="I or L" FT /evidence="ECO:0000269|PubMed:20586483" FT UNSURE 107 FT /note="K or Q" FT /evidence="ECO:0000269|PubMed:20586483" SQ SEQUENCE 108 AA; 11295 MW; 94606DBCF3D93E34 CRC64; AFAGILADAD ITAALAACKA EGTFKHGEFF TKIGLKGKSA ADIKKVFGII DQDKSDFVEE DELKLFLQNF SAGARALTDA ETATFLKAGD SDGDGKIGVD EFAAMVKG //