ID PRVB1_MERHU Reviewed; 108 AA. AC P86764; DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot. DT 05-OCT-2010, sequence version 1. DT 30-NOV-2016, entry version 16. DE RecName: Full=Parvalbumin beta 1 {ECO:0000303|PubMed:20586483}; OS Merluccius hubbsi (Argentine hake) (Merluccius gayi). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata; OC Zeiogadaria; Gadariae; Gadiformes; Gadoidei; Merlucciidae; Merluccius. OX NCBI_TaxID=89949; RN [1] {ECO:0000305} RP PROTEIN SEQUENCE, MASS SPECTROMETRY, AND ACETYLATION AT ALA-1. RC TISSUE=Muscle {ECO:0000269|PubMed:20586483}; RX PubMed=20586483; DOI=10.1021/pr100163e; RA Carrera M., Canas B., Vazquez J., Gallardo J.M.; RT "Extensive de novo sequencing of new parvalbumin isoforms using a RT novel combination of bottom-up proteomics, accurate molecular mass RT measurement by FTICR-MS, and selected MS/MS ion monitoring."; RL J. Proteome Res. 9:4393-4406(2010). CC -!- FUNCTION: In muscle, parvalbumin is thought to be involved in CC relaxation after contraction. It binds two calcium ions (By CC similarity). {ECO:0000250|UniProtKB:P02622, CC ECO:0000250|UniProtKB:P02624}. CC -!- MASS SPECTROMETRY: Mass=11329.748; Mass_error=0.006; CC Method=Electrospray; Range=1-108; CC Evidence={ECO:0000269|PubMed:20586483}; CC -!- MISCELLANEOUS: Is regarded as an important allergen. CC {ECO:0000250|UniProtKB:P02622, ECO:0000269|PubMed:20586483}. CC -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is: CC 4.57, its MW is: 11.30 kDa. {ECO:0000269|PubMed:20586483}. CC -!- SIMILARITY: Belongs to the parvalbumin family. {ECO:0000255}. CC -!- SIMILARITY: Contains 2 EF-hand domains. {ECO:0000255|PROSITE- CC ProRule:PRU00448}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR Allergome; 7644; Mer hu 1. DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro. DR CDD; cd00051; EFh; 1. DR Gene3D; 1.10.238.10; -; 1. DR InterPro; IPR011992; EF-hand-dom_pair. DR InterPro; IPR018247; EF_Hand_1_Ca_BS. DR InterPro; IPR002048; EF_hand_dom. DR InterPro; IPR008080; Parvalbumin. DR PANTHER; PTHR11653; PTHR11653; 1. DR Pfam; PF13499; EF-hand_7; 1. DR SUPFAM; SSF47473; SSF47473; 1. DR PROSITE; PS00018; EF_HAND_1; 2. DR PROSITE; PS50222; EF_HAND_2; 2. PE 1: Evidence at protein level; KW Acetylation; Allergen; Calcium; Direct protein sequencing; KW Metal-binding; Muscle protein; Repeat. FT CHAIN 1 108 Parvalbumin beta 1. FT /FTId=PRO_0000399422. FT DOMAIN 38 73 EF-hand 1. {ECO:0000255|PROSITE- FT ProRule:PRU00448}. FT DOMAIN 77 108 EF-hand 2. {ECO:0000255|PROSITE- FT ProRule:PRU00448}. FT CA_BIND 51 62 1. {ECO:0000255|PROSITE- FT ProRule:PRU00448}. FT CA_BIND 90 101 2. {ECO:0000255|PROSITE- FT ProRule:PRU00448}. FT METAL 51 51 Calcium 1. FT {ECO:0000250|UniProtKB:P02621}. FT METAL 53 53 Calcium 1. FT {ECO:0000250|UniProtKB:P02621}. FT METAL 55 55 Calcium 1. FT {ECO:0000250|UniProtKB:P02621}. FT METAL 57 57 Calcium 1; via carbonyl oxygen. FT {ECO:0000250|UniProtKB:P02621}. FT METAL 59 59 Calcium 1. FT {ECO:0000250|UniProtKB:P02621}. FT METAL 62 62 Calcium 1. FT {ECO:0000250|UniProtKB:P02621}. FT METAL 90 90 Calcium 2. FT {ECO:0000250|UniProtKB:P02621}. FT METAL 92 92 Calcium 2. FT {ECO:0000250|UniProtKB:P02621}. FT METAL 94 94 Calcium 2. FT {ECO:0000250|UniProtKB:P02621}. FT METAL 101 101 Calcium 2. FT {ECO:0000250|UniProtKB:P02621}. FT MOD_RES 1 1 N-acetylalanine. FT {ECO:0000269|PubMed:20586483}. FT UNSURE 5 5 I or L. {ECO:0000269|PubMed:20586483}. FT UNSURE 6 6 L or I. {ECO:0000269|PubMed:20586483}. FT UNSURE 11 11 I or L. {ECO:0000269|PubMed:20586483}. FT UNSURE 15 15 L or I. {ECO:0000269|PubMed:20586483}. FT UNSURE 19 19 K or Q. {ECO:0000269|PubMed:20586483}. FT UNSURE 25 25 K or Q. {ECO:0000269|PubMed:20586483}. FT UNSURE 32 32 K or Q. {ECO:0000269|PubMed:20586483}. FT UNSURE 33 33 I or L. {ECO:0000269|PubMed:20586483}. FT UNSURE 35 35 L or I. {ECO:0000269|PubMed:20586483}. FT UNSURE 36 36 K or Q. {ECO:0000269|PubMed:20586483}. FT UNSURE 38 38 K or Q. {ECO:0000269|PubMed:20586483}. FT UNSURE 43 43 I or L. {ECO:0000269|PubMed:20586483}. FT UNSURE 44 44 K or Q. {ECO:0000269|PubMed:20586483}. FT UNSURE 45 45 K or Q. {ECO:0000269|PubMed:20586483}. FT UNSURE 49 49 I or L. {ECO:0000269|PubMed:20586483}. FT UNSURE 50 50 I or L. {ECO:0000269|PubMed:20586483}. FT UNSURE 52 52 Q or K. {ECO:0000269|PubMed:20586483}. FT UNSURE 54 54 K or Q. {ECO:0000269|PubMed:20586483}. FT UNSURE 63 63 L or I. {ECO:0000269|PubMed:20586483}. FT UNSURE 64 64 K or Q. {ECO:0000269|PubMed:20586483}. FT UNSURE 65 65 L or I. {ECO:0000269|PubMed:20586483}. FT UNSURE 67 67 L or I. {ECO:0000269|PubMed:20586483}. FT UNSURE 68 68 Q or K. {ECO:0000269|PubMed:20586483}. FT UNSURE 77 77 L or I. {ECO:0000269|PubMed:20586483}. FT UNSURE 86 86 L or I. {ECO:0000269|PubMed:20586483}. FT UNSURE 87 87 K or Q. {ECO:0000269|PubMed:20586483}. FT UNSURE 96 96 K or Q. {ECO:0000269|PubMed:20586483}. FT UNSURE 97 97 I or L. {ECO:0000269|PubMed:20586483}. FT UNSURE 107 107 K or Q. {ECO:0000269|PubMed:20586483}. SQ SEQUENCE 108 AA; 11295 MW; 94606DBCF3D93E34 CRC64; AFAGILADAD ITAALAACKA EGTFKHGEFF TKIGLKGKSA ADIKKVFGII DQDKSDFVEE DELKLFLQNF SAGARALTDA ETATFLKAGD SDGDGKIGVD EFAAMVKG //