ID KAX86_MESEU Reviewed; 57 AA. AC P86400; E4VP12; DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot. DT 31-JAN-2018, sequence version 2. DT 22-FEB-2023, entry version 33. DE RecName: Full=Potassium channel toxin alpha-KTx 8.6 {ECO:0000303|PubMed:25792741}; DE AltName: Full=MeuTXKalpha1 {ECO:0000303|PubMed:20889474}; DE AltName: Full=Toxin MeuKTx-1 {ECO:0000303|Ref.4}; DE AltName: Full=meuK-toxin {ECO:0000312|EMBL:AMX81494.1}; DE AltName: Full=pMeKTx1-1 {ECO:0000312|EMBL:AIL48757.1}; DE Flags: Precursor; OS Mesobuthus eupeus (Lesser Asian scorpion) (Buthus eupeus). OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; OC Scorpiones; Buthida; Buthoidea; Buthidae; Mesobuthus. OX NCBI_TaxID=34648; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 29-33, FUNCTION, RP SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND 3D-STRUCTURE MODELING. RC TISSUE=Venom, and Venom gland; RX PubMed=20889474; DOI=10.1074/mcp.m110.002832; RA Zhu S., Peigneur S., Gao B., Luo L., Jin D., Zhao Y., Tytgat J.; RT "Molecular diversity and functional evolution of scorpion potassium channel RT toxins."; RL Mol. Cell. Proteomics 10:M110.002832-M110.002832(2011). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Venom gland; RA Kuzmenkov A.I., Vassilevski A.A., Grishin E.V.; RT "Variability of potassium channel blockers in Mesobuthus eupeus."; RL Submitted (SEP-2013) to the EMBL/GenBank/DDBJ databases. RN [3] RP NUCLEOTIDE SEQUENCE [MRNA]. RC TISSUE=Venom gland; RA Oliw E.H.; RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases. RN [4] RP PROTEIN SEQUENCE OF 29-57, FUNCTION, SUBCELLULAR LOCATION, AND MASS RP SPECTROMETRY. RC TISSUE=Venom; RA Zhu S.Y., Gao B.; RT "Characterization of a potassium channel toxin from the scorpion Mesobuthus RT eupeus venom."; RL Submitted (NOV-2009) to UniProtKB. RN [5] RP NOMENCLATURE. RX PubMed=25792741; DOI=10.1074/jbc.m115.637611; RA Kuzmenkov A.I., Vassilevski A.A., Kudryashova K.S., Nekrasova O.V., RA Peigneur S., Tytgat J., Feofanov A.V., Kirpichnikov M.P., Grishin E.V.; RT "Variability of potassium channel blockers in Mesobuthus eupeus scorpion RT venom with focus on Kv1.1: an integrated transcriptomic and proteomic RT study."; RL J. Biol. Chem. 290:12195-12209(2015). CC -!- FUNCTION: Voltage-gated potassium channel antagonist. Has a high CC potency on Kv1.3/KCNA3, and is moderately active on Kv1.1/KCNA1 (35% CC peak current inhibition at 3 uM of toxin) and on Shaker IR potassium CC channels (70% peak current inhibition at 3 uM of toxin). CC {ECO:0000269|PubMed:20889474, ECO:0000269|Ref.4}. CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20889474}. CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. CC {ECO:0000305|PubMed:20889474}. CC -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to a CC beta-sheet by disulfide bonds (CSalpha/beta). CC {ECO:0000250|UniProtKB:Q9U8D2}. CC -!- MASS SPECTROMETRY: Mass=3251; Method=MALDI; CC Evidence={ECO:0000269|PubMed:20889474, ECO:0000269|Ref.4}; CC -!- MISCELLANEOUS: Negative results: does not inhibit rKv1.1/KCNA1, CC rKv1.2/KCNA2, rKv1.4/KCNA4, rKv1.5/KCNA5, rKv1.6/KCNA6, hKv11.1/KCNH2 CC (when tested at 3 uM). {ECO:0000269|PubMed:20889474}. CC -!- SIMILARITY: Belongs to the short scorpion toxin superfamily. Potassium CC channel inhibitor family. Alpha-KTx 08 subfamily. {ECO:0000255}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; EF442060; ABR20125.1; -; mRNA. DR EMBL; KF612499; AIL48757.1; -; mRNA. DR EMBL; KU569307; AMX81494.1; -; mRNA. DR AlphaFoldDB; P86400; -. DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell. DR GO; GO:0008200; F:ion channel inhibitor activity; IEA:InterPro. DR GO; GO:0015459; F:potassium channel regulator activity; IEA:UniProtKB-KW. DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW. DR InterPro; IPR036574; Scorpion_toxin-like_sf. DR InterPro; IPR008911; Toxin_alpha-KTx_8/9. DR Pfam; PF05453; Toxin_6; 1. DR SUPFAM; SSF57095; Scorpion toxin-like; 1. PE 1: Evidence at protein level; KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin; KW Neurotoxin; Potassium channel impairing toxin; Secreted; Signal; Toxin; KW Voltage-gated potassium channel impairing toxin. FT SIGNAL 1..19 FT /evidence="ECO:0000255" FT PROPEP 20..28 FT /evidence="ECO:0000269|PubMed:20889474" FT /id="PRO_0000442923" FT PEPTIDE 29..57 FT /note="Potassium channel toxin alpha-KTx 8.6" FT /evidence="ECO:0000269|PubMed:20889474" FT /id="PRO_0000401115" FT DISULFID 31..47 FT /evidence="ECO:0000250|UniProtKB:Q9U8D2" FT DISULFID 34..52 FT /evidence="ECO:0000250|UniProtKB:Q9U8D2" FT DISULFID 38..54 FT /evidence="ECO:0000250|UniProtKB:Q9U8D2" SQ SEQUENCE 57 AA; 6435 MW; A6F7559B6214A252 CRC64; MSRLYAIILI ALVFNVIMTI MPDMKVEAVS CEDCPEHCAT KDQRAKCDND KCVCEPK //