ID GDF6A_DANRE Reviewed; 404 AA. AC P85857; DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot. DT 01-JUL-2008, sequence version 1. DT 05-OCT-2010, entry version 20. DE RecName: Full=Growth/differentiation factor 6-A; DE Short=GDF-6-A; DE AltName: Full=Growth differentiation factor 6A; DE AltName: Full=Protein radar; DE Flags: Precursor; GN Name=gdf6a; Synonyms=radar, rdr; OS Danio rerio (Zebrafish) (Brachydanio rerio). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; OC Cyprinidae; Danio. OX NCBI_TaxID=7955; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-56, FUNCTION, AND TISSUE SPECIFICITY. RX PubMed=10415343; DOI=10.1016/S0925-4773(99)00026-X; RA Delot E., Kataoka H., Goutel C., Yan Y.-L., Postlethwait J., RA Wittbrodt J., Rosa F.M.; RT "The BMP-related protein radar: a maintenance factor for dorsal RT neuroectoderm cells?"; RL Mech. Dev. 85:15-25(1999). RN [2] RP NUCLEOTIDE SEQUENCE [MRNA] OF 51-404, FUNCTION, AND TISSUE RP SPECIFICITY. RC TISSUE=Embryo; RX PubMed=7734395; DOI=10.1016/0925-4773(94)00320-M; RA Rissi M., Wittbrodt J., Delot E., Naegeli M., Rosa F.M.; RT "Zebrafish Radar: a new member of the TGF-beta superfamily defines RT dorsal regions of the neural plate and the embryonic retina."; RL Mech. Dev. 49:223-234(1995). RN [3] RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE. RX PubMed=11091070; DOI=10.1016/S0925-4773(00)00470-6; RA Goutel C., Kishimoto Y., Schulte-Merker S., Rosa F.M.; RT "The ventralizing activity of Radar, a maternally expressed bone RT morphogenetic protein, reveals complex bone morphogenetic protein RT interactions controlling dorso-ventral patterning in zebrafish."; RL Mech. Dev. 99:15-27(2000). RN [4] RP FUNCTION, AND TISSUE SPECIFICITY. RX PubMed=12413901; DOI=10.1006/dbio.2002.0794; RA Hall C.J., Flores M.V.C., Davidson A.J., Crosier K.E., Crosier P.S.; RT "Radar is required for the establishment of vascular integrity in the RT zebrafish."; RL Dev. Biol. 251:105-117(2002). RN [5] RP FUNCTION, AND DEVELOPMENTAL STAGE. RX PubMed=12601179; DOI=10.1073/pnas.0530115100; RA Sidi S., Goutel C., Peyrieras N., Rosa F.M.; RT "Maternal induction of ventral fate by zebrafish radar."; RL Proc. Natl. Acad. Sci. U.S.A. 100:3315-3320(2003). RN [6] RP REVIEW. RX PubMed=12682283; DOI=10.1073/pnas.0931010100; RA Wilm T.P., Solnica-Krezel L.; RT "Radar breaks the fog: insights into dorsoventral patterning in RT zebrafish."; RL Proc. Natl. Acad. Sci. U.S.A. 100:4363-4365(2003). CC -!- FUNCTION: Functions maternally in dorsal/ventral patterning to CC induce the expression of the zygotic bmp2b and bmp4 genes and CC ventralize embryos. Zygotic expression does not appear to regulate CC axis specification, but instead functions to establish the CC integrity of the axial vessels during embryonic development. May CC be involved in maintaining the identity of cells of the dorsal- CC most neural tube and of at least a subset of neural crest cells. CC -!- SUBUNIT: Homodimer; disulfide-linked (By similarity). CC -!- SUBCELLULAR LOCATION: Secreted (Probable). CC -!- TISSUE SPECIFICITY: First expressed in late gastrula stage embryos CC (9.5 hours post fertilization (hpf)) in anterior neuroectoderm CC corresponding to the future dorsal part of the brain. Shortly CC after tailbud formation (11 hpf), expression expands to the entire CC neural region and is subsequently expressed in derivatives of the CC lateral neural plate and migrating neural crest cells, with the CC future midbrain and hindbrain showing strong expression. Also CC expressed weakly and transiently in the posterior embryo from 11.5 CC hpf to 15 hpf in the lateral mesoderm, and in ectoderm above the CC neural keel. At 14 hpf, expressed along the entire length of the CC embryo and starting around the 16-somite stage, expressed in the CC dorsal quadrant of the retina, representing the distal tip of the CC eye anlage. At this stage, also expressed in the hatching gland CC and the hypochord. At 24 hpf, expressed in the roof plate CC outlining the fourth brain ventricle, in the posterior hypochord, CC the primitive gut endoderm, the ventral tail mesenchyme, the CC dorsal part of the neural tube and the dorsal fin. Weakly CC expressed in the dorsal part of the posterior spinal cord and in CC blood cell precursors. CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. CC Expressed from early cleavage stage until blastula sphere stage. CC Expression is then barely detectable until the end of gastrulation CC (tailbud stage) when expression is resumed. CC -!- SIMILARITY: Belongs to the TGF-beta family. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR IPI; IPI00488607; -. DR RefSeq; NP_001153466.1; -. DR UniGene; Dr.16432; -. DR ProteinModelPortal; P85857; -. DR SMR; P85857; 300-404. DR Ensembl; ENSDART00000075517; ENSDARP00000069999; ENSDARG00000053479. DR GeneID; 566470; -. DR KEGG; dre:566470; -. DR CTD; 566470; -. DR eggNOG; fiNOG11398; -. DR HOGENOM; HBG717159; -. DR HOVERGEN; HBG107938; -. DR OMA; RRQKYLF; -. DR OrthoDB; EOG9HDWDD; -. DR Bgee; P85857; -. DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell. DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW. DR GO; GO:0040007; P:growth; IEA:InterPro. DR GO; GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW. DR InterPro; IPR001839; TGF-b_C. DR InterPro; IPR001111; TGF-b_N. DR InterPro; IPR015615; TGF-beta-rel. DR InterPro; IPR017948; TGFb_CS. DR PANTHER; PTHR11848; TGFbeta; 1. DR Pfam; PF00019; TGF_beta; 1. DR Pfam; PF00688; TGFb_propeptide; 1. DR SMART; SM00204; TGFB; 1. DR PROSITE; PS00250; TGF_BETA_1; 1. DR PROSITE; PS51362; TGF_BETA_2; 1. PE 2: Evidence at transcript level; KW Cleavage on pair of basic residues; Developmental protein; KW Disulfide bond; Glycoprotein; Growth factor; Secreted; Signal. FT SIGNAL 1 24 Potential. FT PROPEP 25 279 Potential. FT /FTId=PRO_0000342703. FT CHAIN 285 404 Growth/differentiation factor 6-A. FT /FTId=PRO_0000342704. FT CARBOHYD 91 91 N-linked (GlcNAc...) (Potential). FT DISULFID 303 369 By similarity. FT DISULFID 332 401 By similarity. FT DISULFID 336 403 By similarity. FT DISULFID 368 368 Interchain (By similarity). FT CONFLICT 51 51 E -> R (in Ref. 2). FT CONFLICT 70 70 V -> L (in Ref. 2). SQ SEQUENCE 404 AA; 46290 MW; 23233847DF96E197 CRC64; MDALRAVAFY ALFVFLWSLP CCQSAALISQ KRSKGARSAF DGQRSHKFLK EILASSPGAS RRDDFKDPVV PHDYMISIYR TYSAAEKLGL NASFFRSSKS ANTITSFVDK GKDDLTLSPL RRQTYLFDVS TLSDKEELVG AELRIFRKSP GDVQPSPSGV YNLHLLSCRS ERPLASRSID LQDSRKAEWE VLDVWGIFKH RHQENQLCLQ LKVTYGKSDT EIDLKQLGFH RHSRTQQERA ILVVYTRSKK RENLFNEMKE KIKSRGDDDE EESALQFKAR RRRRTALNNR HGKRHGKKSK SRCSKKALHV NFKELGWDDW IIAPLDYEAY HCEGVCDFPL RSHLEPTNHA IIQTLMNSMD PNSTPPSCCV PTKLSPISIL YIDSGNNVVY KQYEDMVVEQ CGCR //